FastQCFastQC Report
Wed 25 May 2016
SRR2088255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354773
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT377991.6052078055931507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT358791.523671283813769No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302081.2828412759956056No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG168170.7141665035228448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123490.5244242226320753No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT112410.4773708548552238No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA106650.4529098983214093No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT102960.4372395980419344No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA92760.3939233208466379No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA82930.3521783203731315No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT79390.337145024170058No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT74460.3162088235256647No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG70320.2986275110169855No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA61850.26265801416951867No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60010.2548440974989946No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT56830.24133961107928448No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA53540.22736798833688002No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA46790.19870280489881617No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46720.19840553632982882No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC44910.19071902047458503No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39270.16676766720189165No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG38160.16205383703652115No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG34900.14820961510939695No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.14748767715614203No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34620.14702054083344762No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31300.13292151727576287No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC30280.12858988955623324No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC29680.12604187325062757No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA28900.12272945205334018No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC27970.11878002677965137No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27410.11640187822775275No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG27120.11517033701337666No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC27000.11466073375225552No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC25540.10846056074194839No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA24770.10519060648308774No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA24530.1041713999608455No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA24320.1032795942538835No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA24280.10310972650017645No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG24190.1027275240543356No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC23580.10013704081030315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82950.030.9336951
GTCGTAC250.005497529.61
TCTCGCG400.001931785423.12500215
TATACGT603.728424E-521.5833324
AGTATCA4000.020.81251
ACGTTTA2000.020.3526
AGCCGTC1303.274181E-1119.92307712
ATTGGAC6100.019.7131143
ATCACGT2000.019.42500123
GTATTGG6350.019.2283461
TCACGTT2050.018.95121824
GTATCAA148150.017.8943652
CGTCTGT9100.017.68681334
GATCACG2350.017.31914922
ACGGCTC2050.017.1463415
ATCCACG1851.8189894E-1217.01
TTGGACC13700.016.8795624
AATGCGT1005.883059E-616.65000235
GGACCCT14050.016.0640566
GTCGACG700.00259357715.85714236
Produced by FastQC (version 0.11.5)