##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2354773
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.014583995994517	31.0	30.0	33.0	27.0	34.0
2	30.834434996494355	31.0	31.0	34.0	27.0	34.0
3	30.85919661895223	31.0	31.0	34.0	27.0	34.0
4	35.01925026318885	35.0	35.0	37.0	32.0	37.0
5	34.92425469461388	35.0	35.0	37.0	32.0	37.0
6	35.026794090130984	36.0	35.0	37.0	32.0	37.0
7	34.666684644337266	35.0	35.0	37.0	31.0	37.0
8	34.88395611806318	35.0	35.0	37.0	32.0	37.0
9	36.28368466939276	38.0	35.0	39.0	32.0	39.0
10	36.22118310342441	38.0	35.0	39.0	32.0	39.0
11	36.313531283057856	38.0	35.0	39.0	32.0	39.0
12	36.06261198000826	38.0	35.0	39.0	30.0	39.0
13	36.23218713650955	38.0	35.0	39.0	32.0	39.0
14	37.10938888801596	39.0	36.0	40.0	31.0	41.0
15	37.164897847902964	39.0	36.0	40.0	31.0	41.0
16	37.06295723621767	39.0	36.0	40.0	31.0	41.0
17	37.195322011930664	39.0	36.0	40.0	32.0	41.0
18	37.222580690368034	39.0	36.0	40.0	32.0	41.0
19	37.31768879632984	39.0	36.0	40.0	31.0	41.0
20	37.28880320948134	39.0	36.0	40.0	31.0	41.0
21	37.231877977197804	39.0	36.0	40.0	31.0	41.0
22	37.15286441623035	39.0	36.0	40.0	31.0	41.0
23	37.039309946224115	39.0	36.0	40.0	31.0	41.0
24	36.95551333398166	39.0	35.0	40.0	31.0	41.0
25	36.87678430150167	39.0	35.0	40.0	31.0	41.0
26	36.606774835621096	38.0	35.0	40.0	30.0	41.0
27	36.43224378740541	38.0	35.0	40.0	30.0	41.0
28	36.252318588670754	38.0	35.0	40.0	30.0	41.0
29	36.084001727555055	38.0	35.0	40.0	30.0	41.0
30	35.89084510481477	38.0	34.0	40.0	29.0	41.0
31	35.59691188917148	38.0	34.0	40.0	29.0	41.0
32	35.38345564519383	38.0	34.0	40.0	27.0	41.0
33	35.43847793396646	38.0	34.0	40.0	28.0	41.0
34	35.41050538629413	38.0	34.0	40.0	27.0	41.0
35	35.276227050335635	38.0	34.0	40.0	27.0	41.0
36	35.16582872319328	38.0	34.0	40.0	26.0	41.0
37	34.98956544855916	38.0	33.0	40.0	25.0	41.0
38	34.83449232686123	38.0	33.0	40.0	25.0	41.0
39	34.73881856127958	38.0	33.0	40.0	24.0	41.0
40	34.516971699607566	38.0	33.0	40.0	23.0	41.0
41	34.3234906294577	38.0	33.0	40.0	22.0	41.0
42	34.084036975113946	38.0	33.0	40.0	21.0	41.0
43	33.714507088369025	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	0.0
15	10.0
16	23.0
17	85.0
18	210.0
19	449.0
20	1014.0
21	1915.0
22	3633.0
23	6207.0
24	10090.0
25	15927.0
26	23997.0
27	34466.0
28	47987.0
29	64419.0
30	81769.0
31	100340.0
32	122593.0
33	148451.0
34	178802.0
35	211636.0
36	257924.0
37	323837.0
38	363168.0
39	355815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.81992022160947	25.687741451086794	14.433747966364486	21.05859036093925
2	17.808722963954487	23.34195270626935	36.36558598217323	22.483738347602934
3	19.932324686923113	26.150206410554222	33.16744331619226	20.750025586330402
4	12.854912129534354	15.988717383798779	34.75583421416842	36.40053627249845
5	10.67134708950714	41.27807648550412	34.38365396579628	13.666922459192458
6	31.23498528308249	35.688832851404364	15.130970161455053	17.9452117040581
7	23.304114664131106	36.852214629605484	20.897725598178678	18.94594510808473
8	28.147468991703235	33.422117545937546	19.996025094563254	18.43438836779596
9	24.73750123684958	13.789736845122652	24.94418782617263	36.528574091855134
10	16.642198632309782	28.563687455223924	33.15173054897436	21.642383363491938
11	30.46280894166869	21.841638238590303	25.596097797961843	22.099455021779168
12	20.101045833292634	28.3292699551082	31.59315144177379	19.976532769825372
13	31.332319505956622	21.339933828016544	29.568922354723785	17.75882431130304
14	20.525630283683398	23.413679365272152	29.334419920731214	26.726270430313242
15	22.824705396231398	29.39909706795517	27.69307275053689	20.083124785276542
16	21.20637530666438	28.645054109249596	28.011744656491306	22.13682592759472
17	18.53350620208402	27.592171304834906	28.42452329799943	25.449799195081653
18	19.65858280182421	26.596576400357915	33.90891606112351	19.835924736694366
19	18.694753167290436	24.925035236942158	35.75567581248808	20.62453578327932
20	24.025627948001784	23.481031929616993	34.06162717170615	18.431712950675074
21	23.22712210476339	24.54109164662581	32.52963236796073	19.702153880650066
22	21.053281993635906	24.424519900644352	33.39162628414714	21.130571821572612
23	20.154002105510806	26.78139251639118	34.637436389834605	18.42716898826341
24	18.270720787099222	25.72893438136075	32.97273240350556	23.027612428034463
25	19.7891686374865	26.82241557891143	34.450921596264266	18.9374941873378
26	21.508570040509213	25.41238582232767	32.712112802380524	20.36693133478259
27	19.96948325804653	27.13374070451802	32.199834124138505	20.69694191329695
28	19.556237480215717	26.71348788184679	32.159830268140496	21.570444369797002
29	18.377312802550392	26.71174673737129	33.3246134553097	21.586327004768613
30	17.693255358372124	26.83685433797653	35.981472524103175	19.48841777954818
31	19.47958465635541	26.120819289162906	34.24967077505985	20.149925279421836
32	20.847020073697127	26.547144884029162	31.936454172015733	20.669380870257985
33	22.002375600535593	26.40318196276244	31.156506380869832	20.437936055832136
34	19.757997904681257	29.81493332903002	31.271591784006358	19.15547698228237
35	18.092232244891544	30.823268315035037	31.5052448792304	19.579254560843022
36	19.31702121605777	26.32894975439246	34.34725979956455	20.006769229985224
37	19.345941201126394	26.842502440787285	33.05456619385393	20.75699016423239
38	19.88433704650087	26.342284373058465	33.71454488394423	20.058833696496436
39	21.227863577508323	25.61503805250018	33.45447735301874	19.70262101697276
40	19.026122687834455	26.58888988450267	32.50861123343949	21.876376194223393
41	20.143640172534678	25.99664596120305	32.385159843432895	21.474554022829377
42	17.17995747360786	27.92260655273353	32.96479108601976	21.93264488763885
43	17.88172363111009	27.671329678062385	32.56878688519021	21.878159805637313
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	289.0
2	383.0
3	1058.5
4	1734.0
5	1734.0
6	3107.0
7	4480.0
8	5164.0
9	5848.0
10	8976.5
11	12105.0
12	12105.0
13	22407.0
14	32709.0
15	55530.0
16	78351.0
17	74974.5
18	71598.0
19	71598.0
20	76444.0
21	81290.0
22	64611.0
23	47932.0
24	51958.5
25	55985.0
26	55985.0
27	59357.5
28	62730.0
29	63681.5
30	64633.0
31	68214.0
32	71795.0
33	71795.0
34	76919.5
35	82044.0
36	89460.0
37	96876.0
38	99743.5
39	102611.0
40	102611.0
41	107248.5
42	111886.0
43	131879.5
44	151873.0
45	200583.5
46	249294.0
47	249294.0
48	254037.0
49	258780.0
50	230953.5
51	203127.0
52	185440.0
53	167753.0
54	167753.0
55	142240.0
56	116727.0
57	91639.0
58	66551.0
59	56354.0
60	46157.0
61	46157.0
62	40246.0
63	34335.0
64	29979.5
65	25624.0
66	21778.5
67	17933.0
68	17933.0
69	15245.0
70	12557.0
71	10277.5
72	7998.0
73	6457.5
74	4917.0
75	4917.0
76	3791.5
77	2666.0
78	2100.0
79	1534.0
80	1220.5
81	907.0
82	907.0
83	659.0
84	411.0
85	320.0
86	229.0
87	179.0
88	129.0
89	129.0
90	93.0
91	57.0
92	37.0
93	17.0
94	14.0
95	11.0
96	11.0
97	6.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2354773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.22756780967575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.38947325342919	25.862669831856387
2	13.905030198641107	10.074908488337195
3	5.7057425453144415	6.2011552489479245
4	2.791951290470886	4.04582418787383
5	1.5833187949843497	2.867989450481481
6	1.0556878028954202	2.294700087914486
7	0.6698659480572045	1.6987329839644576
8	0.47137297786683174	1.3661357215455547
9	0.3345457098122233	1.0907799648892582
>10	1.7839974804075842	12.09485535589937
>50	0.16804051328990494	4.246003866897906
>100	0.11755903088781555	8.187264358094435
>500	0.010815813750395678	2.6417995858506855
>1k	0.010578103558079291	7.489950062899581
>5k	0.0010696958654237484	2.721672751932001
>10k+	9.508407692655542E-4	7.115558052615464
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37799	1.6052078055931507	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35879	1.523671283813769	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30208	1.2828412759956056	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16817	0.7141665035228448	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12349	0.5244242226320753	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11241	0.4773708548552238	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10665	0.4529098983214093	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10296	0.4372395980419344	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9276	0.3939233208466379	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8293	0.3521783203731315	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7939	0.337145024170058	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7446	0.3162088235256647	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7032	0.2986275110169855	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6185	0.26265801416951867	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6001	0.2548440974989946	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5683	0.24133961107928448	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5354	0.22736798833688002	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4679	0.19870280489881617	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4672	0.19840553632982882	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4491	0.19071902047458503	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3927	0.16676766720189165	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3816	0.16205383703652115	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3490	0.14820961510939695	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3473	0.14748767715614203	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3462	0.14702054083344762	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3130	0.13292151727576287	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	3028	0.12858988955623324	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2968	0.12604187325062757	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2890	0.12272945205334018	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2797	0.11878002677965137	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2741	0.11640187822775275	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2712	0.11517033701337666	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2700	0.11466073375225552	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2554	0.10846056074194839	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2477	0.10519060648308774	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2453	0.1041713999608455	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2432	0.1032795942538835	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	2428	0.10310972650017645	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	2419	0.1027275240543356	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	2358	0.10013704081030315	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.24669384267613E-5	0.0	0.0	0.0
3	0.0	4.24669384267613E-5	0.0	0.0	0.0
4	0.0	4.24669384267613E-5	0.0	0.0	0.0
5	0.0	4.24669384267613E-5	0.0	0.0	0.0
6	4.24669384267613E-5	4.24669384267613E-5	0.0	4.24669384267613E-5	0.0
7	4.24669384267613E-5	4.24669384267613E-5	0.0	4.24669384267613E-5	0.0
8	4.24669384267613E-5	4.24669384267613E-5	0.0	8.49338768535226E-5	0.0
9	4.24669384267613E-5	4.24669384267613E-5	0.0	2.548016305605678E-4	0.0
10	4.24669384267613E-5	8.49338768535226E-5	0.0	4.2466938426761303E-4	0.0
11	4.24669384267613E-5	8.49338768535226E-5	0.0	4.6713632269437436E-4	0.0
12	4.24669384267613E-5	1.274008152802839E-4	0.0	6.370040764014196E-4	0.0
13	4.24669384267613E-5	1.274008152802839E-4	0.0	8.068718301084648E-4	0.0
14	4.24669384267613E-5	1.274008152802839E-4	0.0	9.7673958381551E-4	0.0
15	4.24669384267613E-5	1.274008152802839E-4	0.0	0.0015712767217901684	0.0
16	4.24669384267613E-5	1.274008152802839E-4	0.0	0.0026329501824592007	0.0
17	4.24669384267613E-5	1.274008152802839E-4	0.0	0.004204226904249369	0.0
18	4.24669384267613E-5	1.274008152802839E-4	0.0	0.004968631795931073	0.0
19	4.24669384267613E-5	1.274008152802839E-4	0.0	0.005987838318173344	0.0
20	4.24669384267613E-5	1.274008152802839E-4	0.0	0.0073043134094029445	0.0
21	4.24669384267613E-5	1.274008152802839E-4	0.0	0.009342726453887487	0.0
22	4.24669384267613E-5	1.274008152802839E-4	0.0	0.016477172109583386	0.0
23	4.24669384267613E-5	1.274008152802839E-4	0.0	0.025437696117630023	0.0
24	4.24669384267613E-5	1.274008152802839E-4	0.0	0.041150463335531705	0.0
25	4.24669384267613E-5	1.274008152802839E-4	0.0	0.04909178082133607	0.0
26	4.24669384267613E-5	1.274008152802839E-4	0.0	0.06089758970397571	0.0
27	4.24669384267613E-5	1.274008152802839E-4	0.0	0.14748767715614203	0.0
28	4.24669384267613E-5	1.274008152802839E-4	0.0	0.23522437194583087	0.0
29	4.24669384267613E-5	1.274008152802839E-4	0.0	0.3365929539705101	0.0
30	4.24669384267613E-5	1.274008152802839E-4	0.0	0.5318134699183318	0.0
31	4.24669384267613E-5	1.274008152802839E-4	0.0	0.7939194138883026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8295	0.0	30.933695	1
GTCGTAC	25	0.0054975	29.6	1
TCTCGCG	40	0.0019317854	23.125002	15
TATACGT	60	3.728424E-5	21.583332	4
AGTATCA	400	0.0	20.8125	1
ACGTTTA	200	0.0	20.35	26
AGCCGTC	130	3.274181E-11	19.923077	12
ATTGGAC	610	0.0	19.713114	3
ATCACGT	200	0.0	19.425001	23
GTATTGG	635	0.0	19.228346	1
TCACGTT	205	0.0	18.951218	24
GTATCAA	14815	0.0	17.894365	2
CGTCTGT	910	0.0	17.686813	34
GATCACG	235	0.0	17.319149	22
ACGGCTC	205	0.0	17.146341	5
ATCCACG	185	1.8189894E-12	17.0	1
TTGGACC	1370	0.0	16.879562	4
AATGCGT	100	5.883059E-6	16.650002	35
GGACCCT	1405	0.0	16.064056	6
GTCGACG	70	0.002593577	15.857142	36
>>END_MODULE