FastQCFastQC Report
Wed 25 May 2016
SRR2088254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032450
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT167831.6255508741343405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT154561.497021647537411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129541.2546854569228534No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG76740.7432805462734272No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53890.5219623226306359No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT49780.4821540994721294No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA49710.4814761005375563No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT44710.4330476052109061No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42390.4105767833793404No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA37340.3616640030994237No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT36540.35391544384715967No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG32750.3172066443895588No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT32280.3126543658288537No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA29290.28369412562351687No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA26980.2613201607826045No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA26330.25502445639014No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT24400.23633105719405295No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20380.19739454695142622No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.1865465639982566No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC18820.18228485640951134No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17360.1681437357741295No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG16580.16058889050317207No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16220.15710203883965326No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT15830.15332461620417454No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.14150806334447188No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC14350.13898978158748607No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA12680.12281466414838492No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC12670.12271780715773162No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG12560.1216523802605453No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.12145866627923871No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC12260.11874667054094629No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA11980.11603467480265388No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC11760.11390382100828127No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC11510.11148239624194876No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA11230.10877040050365636No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT10850.10508983485883094No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC10830.10489612087752434No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA10750.10412126495229794No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC10680.10344326601772483No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG10660.10324955203641824No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG10370.10044069930747251No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA10350.10024698532616592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC200.001841849437.01
GGTATCA35850.030.3430981
GCGTATT250.005496088429.5999989
CGAACTA358.868782E-426.4285724
GTATTGG3250.022.769231
TATACCG652.682409E-622.7692285
CGCAACC502.7018448E-422.19999910
ATTGGAC3050.021.8360653
CTAACCT705.101094E-621.1428574
TTGGACC5700.020.1228074
TATTGGA3700.020.0000022
CGTCTGT3500.019.02857234
ACGATCT1002.8742215E-718.49999832
AGCGAAC500.007034410718.49999822
GTATCAA61400.018.2288272
GGACCCT6400.017.6328126
GCTAAAC650.001579787217.0769213
GACCCTC6650.016.691737
CGCCGGA803.3821486E-416.187514
TGGACCC7050.016.0070935
Produced by FastQC (version 0.11.5)