##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088254_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1032450
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.019277446849728	31.0	30.0	33.0	27.0	34.0
2	30.82704828320984	31.0	31.0	34.0	27.0	34.0
3	30.793889292459685	31.0	31.0	34.0	27.0	34.0
4	34.9776279722989	35.0	35.0	37.0	32.0	37.0
5	34.900081359872146	35.0	35.0	37.0	32.0	37.0
6	35.01754951813647	36.0	35.0	37.0	32.0	37.0
7	34.628558283694126	35.0	35.0	37.0	30.0	37.0
8	34.86580367087995	35.0	35.0	37.0	32.0	37.0
9	36.24581916799845	38.0	35.0	39.0	32.0	39.0
10	36.19915637561141	38.0	35.0	39.0	32.0	39.0
11	36.30206595961064	38.0	35.0	39.0	32.0	39.0
12	36.03444137730641	38.0	35.0	39.0	30.0	39.0
13	36.20374255411885	38.0	35.0	39.0	32.0	39.0
14	37.06820766138796	39.0	36.0	40.0	31.0	41.0
15	37.10483219526369	39.0	36.0	40.0	31.0	41.0
16	37.008429463896555	38.0	36.0	40.0	31.0	41.0
17	37.14797907889002	39.0	36.0	40.0	31.0	41.0
18	37.17452176860865	39.0	36.0	40.0	31.0	41.0
19	37.26803816165432	39.0	36.0	40.0	31.0	41.0
20	37.24900479442104	39.0	36.0	40.0	31.0	41.0
21	37.1783989539445	39.0	36.0	40.0	31.0	41.0
22	37.10762361373432	39.0	36.0	40.0	31.0	41.0
23	36.97502445639014	39.0	35.0	40.0	31.0	41.0
24	36.89589907501574	39.0	35.0	40.0	31.0	41.0
25	36.81177974720325	38.0	35.0	40.0	31.0	41.0
26	36.53919221269795	38.0	35.0	40.0	30.0	41.0
27	36.382706184318856	38.0	35.0	40.0	30.0	41.0
28	36.20677805220592	38.0	35.0	40.0	30.0	41.0
29	36.035301467383405	38.0	35.0	40.0	30.0	41.0
30	35.832260157876895	38.0	34.0	40.0	29.0	41.0
31	35.54983098455131	38.0	34.0	40.0	28.0	41.0
32	35.2998217831372	37.0	34.0	40.0	27.0	41.0
33	35.37655867112209	38.0	34.0	40.0	27.0	41.0
34	35.341993316867644	38.0	34.0	40.0	27.0	41.0
35	35.222886338321466	38.0	34.0	40.0	27.0	41.0
36	35.12007167417308	38.0	34.0	40.0	26.0	41.0
37	34.944036030800525	38.0	33.0	40.0	25.0	41.0
38	34.78965276768851	38.0	33.0	40.0	25.0	41.0
39	34.70593830209695	38.0	33.0	40.0	24.0	41.0
40	34.48869678919076	38.0	33.0	40.0	23.0	41.0
41	34.28264032156521	38.0	33.0	40.0	22.0	41.0
42	34.02450966148482	37.0	33.0	40.0	21.0	41.0
43	33.66274589568502	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	4.0
16	10.0
17	27.0
18	85.0
19	205.0
20	415.0
21	816.0
22	1535.0
23	2652.0
24	4289.0
25	6944.0
26	10374.0
27	15329.0
28	21417.0
29	28301.0
30	36331.0
31	44613.0
32	54764.0
33	66314.0
34	79465.0
35	94482.0
36	114402.0
37	144762.0
38	160088.0
39	144825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.13467964550341	25.349121022809822	14.283597268632864	21.2326020630539
2	18.05956704925178	23.243740616979032	36.08939900237299	22.607293331396193
3	20.08097244418616	26.211923095549423	32.76216765945082	20.9449368008136
4	12.982033028233813	15.781587486076806	34.630829580124946	36.60554990556443
5	10.8026538815439	41.03656351397162	34.440893021453824	13.719889583030657
6	31.70545789142331	35.38834810402441	14.873165770739503	18.033028233812775
7	23.681437357741295	36.4255896169306	20.865901496440507	19.0270715288876
8	28.352850016949972	33.29623710591312	19.811806867160637	18.53910600997627
9	24.84275267567437	13.683568211535668	24.550341420892053	36.92333769189791
10	16.742408833357548	28.26935929100683	33.10416969344763	21.884062182188
11	30.692818054143057	21.5891326456487	25.473291684827355	22.244757615380887
12	20.342098890987454	28.181800571456243	31.37459441135164	20.10150612620466
13	31.631943435517456	20.865417211487237	29.390575814809434	18.11206353818587
14	20.832970119618384	23.072788028475955	28.979224175504868	27.115017676400793
15	23.08441086735435	29.018548113710107	27.504479635817713	20.392561383117826
16	21.616252603031626	28.21686280207274	27.729284711124023	22.43759988377161
17	18.784929052254345	27.351058162622888	28.200106542689717	25.66390624243305
18	20.04164850598092	26.254249600464913	33.57508838200397	20.129013511550195
19	19.081020872681485	24.600610199041114	35.419439198024115	20.898929730253283
20	24.35498087074435	23.1680953072788	33.76802750738535	18.708896314591506
21	23.52598188774275	24.275751852389945	32.260835875829336	19.937430384037967
22	21.365199283258267	24.13434064603613	33.041309506513635	21.45915056419197
23	20.409511356482156	26.67993607438617	34.24088333575476	18.66966923337692
24	18.676933507675916	25.439682309070655	32.5515037047799	23.331880478473536
25	20.033609375756694	26.564966826480703	34.10944839943823	19.291975398324375
26	21.75349895878735	25.168967020194682	32.3376434694174	20.739890551600563
27	20.23536248728752	27.01012155552327	31.713109593684923	21.041406363504287
28	19.901205869533634	26.501138069640177	31.91815584289796	21.67950021792823
29	18.83636011429125	26.349653736258418	33.05758148094339	21.75640466850695
30	18.034771659644537	26.610392755097102	35.614702891181174	19.740132694077197
31	19.935686958206208	25.82449513293622	33.940432950748225	20.29938495810935
32	21.26194973122185	26.263644728558283	31.555523269892007	20.91888227032786
33	22.2229647924839	26.22509564627827	30.918107414402634	20.6338321468352
34	20.194585694222482	29.465058840621822	31.072884885466607	19.26747057968909
35	18.350912877136906	30.757034238946197	31.189016417259918	19.703036466656982
36	19.767736936413385	26.273620998595575	33.93413724635575	20.024504818635286
37	19.74410383069398	26.83374497554361	32.65300983098455	20.76914136277786
38	20.26122330379195	26.266163010315267	33.3045668071093	20.16804687878348
39	21.579059518620756	25.50515763475229	33.05273863141072	19.863044215216235
40	19.345343600174342	26.67935493244225	31.98624630732723	21.98905516005618
41	20.582110513826336	26.008523415177493	31.848709380599544	21.56065669039663
42	17.683665068526324	27.970555474841397	32.43130417937915	21.914475277253135
43	18.384909680856214	27.779940917235702	32.01317255072885	21.821976851179233
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	99.0
1	132.5
2	166.0
3	453.5
4	741.0
5	741.0
6	1396.5
7	2052.0
8	2277.0
9	2502.0
10	3845.5
11	5189.0
12	5189.0
13	9779.0
14	14369.0
15	24052.5
16	33736.0
17	31811.0
18	29886.0
19	29886.0
20	32346.0
21	34806.0
22	27202.0
23	19598.0
24	20887.0
25	22176.0
26	22176.0
27	23785.5
28	25395.0
29	25713.0
30	26031.0
31	27899.5
32	29768.0
33	29768.0
34	32217.5
35	34667.0
36	37562.0
37	40457.0
38	41917.0
39	43377.0
40	43377.0
41	46162.5
42	48948.0
43	58079.0
44	67210.0
45	88549.0
46	109888.0
47	109888.0
48	112316.0
49	114744.0
50	102797.5
51	90851.0
52	83562.0
53	76273.0
54	76273.0
55	64875.0
56	53477.0
57	42046.5
58	30616.0
59	26236.5
60	21857.0
61	21857.0
62	19159.0
63	16461.0
64	14435.0
65	12409.0
66	10540.5
67	8672.0
68	8672.0
69	7334.5
70	5997.0
71	5117.0
72	4237.0
73	3371.0
74	2505.0
75	2505.0
76	1979.0
77	1453.0
78	1162.5
79	872.0
80	668.0
81	464.0
82	464.0
83	358.5
84	253.0
85	180.0
86	107.0
87	93.0
88	79.0
89	79.0
90	63.0
91	47.0
92	29.0
93	11.0
94	7.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1032450.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.80463791290488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.21257701159351	34.5948155532673
2	12.33295525724646	11.051471893939686
3	4.263004920052354	5.730071755916332
4	1.9556428963652819	3.50487487434364
5	1.0676021126341282	2.39167630458122
6	0.6379295251867894	1.7149320833967259
7	0.4254093090801671	1.3342217040681148
8	0.31828992282981666	1.1408691794973096
9	0.22206995151172124	0.895478739193711
>10	1.3666297823876823	11.35998741721037
>50	0.10772179902262578	3.304662855951797
>100	0.07042280143769727	6.257850136817497
>500	0.010311126673743795	3.3273825835816457
>1k	0.008336655608558813	7.665433913267881
>5k	4.387713478188849E-4	1.2840270210430131
>10k+	6.581570217283273E-4	4.442243983923823
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16783	1.6255508741343405	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15456	1.497021647537411	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12954	1.2546854569228534	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7674	0.7432805462734272	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5389	0.5219623226306359	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4978	0.4821540994721294	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4971	0.4814761005375563	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4471	0.4330476052109061	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4239	0.4105767833793404	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3734	0.3616640030994237	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3654	0.35391544384715967	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3275	0.3172066443895588	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3228	0.3126543658288537	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2929	0.28369412562351687	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2698	0.2613201607826045	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2633	0.25502445639014	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2440	0.23633105719405295	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2038	0.19739454695142622	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1926	0.1865465639982566	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1882	0.18228485640951134	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1736	0.1681437357741295	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1658	0.16058889050317207	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1622	0.15710203883965326	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1583	0.15332461620417454	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1461	0.14150806334447188	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1435	0.13898978158748607	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1268	0.12281466414838492	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1267	0.12271780715773162	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1256	0.1216523802605453	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1254	0.12145866627923871	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1226	0.11874667054094629	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1198	0.11603467480265388	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1176	0.11390382100828127	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1151	0.11148239624194876	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1123	0.10877040050365636	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	1085	0.10508983485883094	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	1083	0.10489612087752434	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1075	0.10412126495229794	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	1068	0.10344326601772483	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	1066	0.10324955203641824	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	1037	0.10044069930747251	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1035	0.10024698532616592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.68569906533004E-5	0.0	0.0	0.0
3	0.0	9.68569906533004E-5	0.0	0.0	0.0
4	0.0	9.68569906533004E-5	0.0	0.0	0.0
5	0.0	9.68569906533004E-5	0.0	0.0	0.0
6	9.68569906533004E-5	9.68569906533004E-5	0.0	0.0	0.0
7	9.68569906533004E-5	9.68569906533004E-5	0.0	0.0	0.0
8	9.68569906533004E-5	9.68569906533004E-5	0.0	0.0	0.0
9	9.68569906533004E-5	9.68569906533004E-5	0.0	0.0	0.0
10	9.68569906533004E-5	9.68569906533004E-5	0.0	4.84284953266502E-4	0.0
11	9.68569906533004E-5	9.68569906533004E-5	0.0	6.779989345731028E-4	0.0
12	9.68569906533004E-5	9.68569906533004E-5	0.0	6.779989345731028E-4	0.0
13	9.68569906533004E-5	9.68569906533004E-5	0.0	6.779989345731028E-4	0.0
14	9.68569906533004E-5	9.68569906533004E-5	0.0	9.68569906533004E-4	0.0
15	9.68569906533004E-5	9.68569906533004E-5	0.0	0.0012591408784929053	0.0
16	9.68569906533004E-5	9.68569906533004E-5	0.0	0.002905709719599012	0.0
17	9.68569906533004E-5	9.68569906533004E-5	0.0	0.0045522785607051185	0.0
18	9.68569906533004E-5	9.68569906533004E-5	0.0	0.005133420504624921	0.0
19	9.68569906533004E-5	9.68569906533004E-5	0.0	0.006392561383117826	0.0
20	9.68569906533004E-5	9.68569906533004E-5	0.0	0.008620272168143735	0.0
21	9.68569906533004E-5	9.68569906533004E-5	0.0	0.011913409850355949	0.0
22	9.68569906533004E-5	9.68569906533004E-5	0.0	0.020533682018499685	0.0
23	9.68569906533004E-5	9.68569906533004E-5	0.0	0.03089738001840283	0.0
24	9.68569906533004E-5	9.68569906533004E-5	0.0	0.0486222093079568	0.0
25	9.68569906533004E-5	9.68569906533004E-5	0.0	0.057436195457407135	0.0
26	9.68569906533004E-5	9.68569906533004E-5	0.0	0.06779989345731029	0.0
27	9.68569906533004E-5	9.68569906533004E-5	0.0	0.13492178798004745	0.0
28	9.68569906533004E-5	9.68569906533004E-5	0.0	0.21076081166158167	0.0
29	9.68569906533004E-5	9.68569906533004E-5	0.0	0.29986924306261803	0.0
30	9.68569906533004E-5	9.68569906533004E-5	0.0	0.4717904014722263	0.0
31	9.68569906533004E-5	9.68569906533004E-5	0.0	0.7018257542738147	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGC	20	0.0018418494	37.0	1
GGTATCA	3585	0.0	30.343098	1
GCGTATT	25	0.0054960884	29.599998	9
CGAACTA	35	8.868782E-4	26.42857	24
GTATTGG	325	0.0	22.76923	1
TATACCG	65	2.682409E-6	22.769228	5
CGCAACC	50	2.7018448E-4	22.199999	10
ATTGGAC	305	0.0	21.836065	3
CTAACCT	70	5.101094E-6	21.142857	4
TTGGACC	570	0.0	20.122807	4
TATTGGA	370	0.0	20.000002	2
CGTCTGT	350	0.0	19.028572	34
ACGATCT	100	2.8742215E-7	18.499998	32
AGCGAAC	50	0.0070344107	18.499998	22
GTATCAA	6140	0.0	18.228827	2
GGACCCT	640	0.0	17.632812	6
GCTAAAC	65	0.0015797872	17.076921	3
GACCCTC	665	0.0	16.69173	7
CGCCGGA	80	3.3821486E-4	16.1875	14
TGGACCC	705	0.0	16.007093	5
>>END_MODULE