FastQCFastQC Report
Wed 25 May 2016
SRR2088253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742000
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63250.8524258760107817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55250.7446091644204852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46600.628032345013477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22460.3026954177897574No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG19080.2571428571428571No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT14350.19339622641509432No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT12790.17237196765498652No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA10390.1400269541778976No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA10170.13706199460916443No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT9540.12857142857142856No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT9010.12142857142857141No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9010.12142857142857141No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG8380.11293800539083558No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.1049865229110512No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA7580.10215633423180594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACT551.853914E-830.2727284
GCGTCCA250.00549510329.69
GGTATCA16950.026.8495581
CGAATTA450.003824548620.55555715
CGGACCA555.140733E-420.1818189
CGTCTGT1303.274181E-1119.92307734
CGTATAC500.00703258918.53
CTGTGCG902.1501237E-618.59
TAGTACG500.00703258918.54
GTATCAA28050.016.8181822
ATTGGAC1107.798426E-716.8181823
ATAACAC1059.334099E-615.8571423
TGTACTG1302.5851477E-715.6538465
TACACTG1302.5851477E-715.6538465
CCTAGTA1101.4504105E-515.1363642
GTACTGT1353.9663246E-715.0740756
TTCGTTT750.004102582614.810
GTAGGAC750.004102582614.83
AGTATAC908.2691404E-414.3888893
CACTGTG2405.456968E-1213.8749997
Produced by FastQC (version 0.11.5)