FastQCFastQC Report
Wed 25 May 2016
SRR2088252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835941
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT261751.425699409730487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT235701.2838103185233076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208621.1363110252453645No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG135220.7365160427268632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86930.4734901611762034No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT82620.45001446124902705No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA77490.4220723868577476No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT72860.3968537115299457No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA71320.3884656424144349No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA60390.32893213888681605No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT57760.31460705981292425No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT54470.2966870939752421No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG53590.29189391162352163No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA46880.2553458961916532No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA46750.25463781243514905No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA40180.21885234874105433No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT39490.2150940580334553No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA32610.17762008692000453No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC32330.1760949834444571No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32200.17538689968795296No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG28970.1577937417378881No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27730.15103971206046382No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG27200.14815290905317763No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26180.14259717496368346No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT24160.13159464274723426No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23290.12685592837678336No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC21980.11972062283047223No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG21890.11923041099904626No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC21350.11628914001049052No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA21110.11498190846002132No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC20540.11187723352765694No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA20210.11007979014576177No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.10757426300736243No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC18530.10092916929247728No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA18490.10071129736739907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62500.029.4520021
TAGGTCG551.9034624E-523.54545421
CGGTTAC400.001931624623.12531
ATTGGAC6700.022.3656713
GTATTGG6050.022.016531
CGGGATA1107.4578566E-1121.86363624
AGCCGTC1251.8189894E-1120.72000112
TCACGTT851.2461369E-619.58823624
TTGGACC11800.019.4406784
ACGTTTA902.1536416E-618.526
TACGTAG500.007036444718.49999814
TATTGGA7750.018.3806462
TGGACCC12150.018.2716055
GTATCAA106450.017.935182
GGACCCT12350.017.8259126
GACCCTC12500.017.4647
AGTCCGA650.001580419817.07692310
CGTCTGT7400.016.7534
AAGCCGT1606.2937033E-1016.187511
TCTATAC1851.8189894E-1116.03
Produced by FastQC (version 0.11.5)