##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088252_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1835941
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.847864936836206	31.0	30.0	33.0	27.0	34.0
2	30.76128916996788	31.0	31.0	34.0	27.0	34.0
3	30.764204296325428	31.0	31.0	34.0	27.0	34.0
4	34.98154515858625	35.0	35.0	37.0	32.0	37.0
5	34.914412826991715	35.0	35.0	37.0	32.0	37.0
6	35.038992538431245	36.0	35.0	37.0	32.0	37.0
7	34.63819970249589	35.0	35.0	37.0	30.0	37.0
8	34.873353773351106	35.0	35.0	37.0	32.0	37.0
9	36.267886604199155	38.0	35.0	39.0	32.0	39.0
10	36.22317438305479	38.0	35.0	39.0	32.0	39.0
11	36.32385899111137	38.0	35.0	39.0	32.0	39.0
12	36.05483564014312	38.0	35.0	39.0	30.0	39.0
13	36.217656231872375	38.0	35.0	39.0	32.0	39.0
14	37.1109610820827	39.0	36.0	40.0	31.0	41.0
15	37.152925938251826	39.0	36.0	40.0	31.0	41.0
16	37.06933937419558	39.0	36.0	40.0	31.0	41.0
17	37.194736105354146	39.0	36.0	40.0	32.0	41.0
18	37.208134139386836	39.0	36.0	40.0	32.0	41.0
19	37.300318474286485	39.0	36.0	40.0	31.0	41.0
20	37.26297304760883	39.0	36.0	40.0	31.0	41.0
21	37.198135996744995	39.0	36.0	40.0	31.0	41.0
22	37.11976256317605	39.0	36.0	40.0	31.0	41.0
23	36.98303758127304	39.0	35.0	40.0	31.0	41.0
24	36.90676770114072	39.0	35.0	40.0	31.0	41.0
25	36.824162105427135	39.0	35.0	40.0	31.0	41.0
26	36.56055396115671	38.0	35.0	40.0	30.0	41.0
27	36.41565115654588	38.0	35.0	40.0	30.0	41.0
28	36.24256117162806	38.0	35.0	40.0	30.0	41.0
29	36.08084573523877	38.0	35.0	40.0	30.0	41.0
30	35.90418319542948	38.0	34.0	40.0	29.0	41.0
31	35.62061743814208	38.0	34.0	40.0	28.0	41.0
32	35.377646122615054	38.0	34.0	40.0	27.0	41.0
33	35.465398942558615	38.0	34.0	40.0	28.0	41.0
34	35.42549079736222	38.0	34.0	40.0	27.0	41.0
35	35.31750965853478	38.0	34.0	40.0	27.0	41.0
36	35.21971403220474	38.0	34.0	40.0	26.0	41.0
37	35.04948688438245	38.0	33.0	40.0	25.0	41.0
38	34.90838104274593	38.0	33.0	40.0	25.0	41.0
39	34.83949102939582	38.0	33.0	40.0	24.0	41.0
40	34.64562368834292	38.0	33.0	40.0	24.0	41.0
41	34.45959047703602	38.0	33.0	40.0	23.0	41.0
42	34.23265671391401	38.0	33.0	40.0	22.0	41.0
43	33.890169673208455	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	4.0
16	21.0
17	56.0
18	146.0
19	348.0
20	694.0
21	1471.0
22	2629.0
23	4751.0
24	7670.0
25	12065.0
26	18165.0
27	26371.0
28	36980.0
29	49935.0
30	64034.0
31	78878.0
32	95744.0
33	115872.0
34	139874.0
35	166132.0
36	202682.0
37	254693.0
38	286471.0
39	270253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.06400042267153	25.618633714264238	13.818908123953873	21.498457739110354
2	17.95831129649591	22.98222001687418	36.21723138161847	22.842237305011437
3	19.873024242064425	26.478792074472977	32.44445219100178	21.203731492460815
4	12.962017842621304	15.863200396962648	34.936689141971335	36.23809261844471
5	10.927311934315972	40.86008210503497	34.62780122019172	13.584804740457345
6	31.548617303061484	35.53480204429228	15.287582770906036	17.628997881740208
7	23.931760334346254	35.955294859693204	21.251881187903095	18.861063618057443
8	28.022632535577124	33.41616097685056	20.04911922550888	18.51208726206343
9	24.93369884979964	13.749842723704084	24.447190841100014	36.869267585396265
10	16.503852792655103	28.065117560967373	33.535173515924534	21.89585613045299
11	30.809432329252413	21.692036944542338	25.53932833353577	21.95920239266948
12	20.14950371498866	27.755031343599825	31.696116596339426	20.39934834507209
13	31.18896522273864	20.973331931690613	29.380029096795596	18.457673748775154
14	20.823381579255543	23.09153725528217	28.746893282518336	27.33818788294395
15	23.081569614709842	28.972608596899356	27.39646862290237	20.549353165488434
16	21.668996988465317	28.47852953880326	27.671041716482176	22.181431756249246
17	18.88453931798462	27.357524016294644	28.179010109802004	25.578926555918734
18	20.154460301284193	26.39322287589852	33.17165420893155	20.28066261388574
19	19.225399944769467	24.870189183639344	34.77442902576935	21.129981845821845
20	24.29620559702082	23.531638543940137	33.29545993035724	18.876695928681805
21	23.383213294980614	24.6861963429108	31.81807040640195	20.112519955706638
22	21.47073353664415	24.247129945896955	32.848822483946925	21.433314033511973
23	20.523535342366667	26.480643985836146	34.046192116195456	18.949628555601734
24	18.93279794938944	25.59673758579388	32.12684939221903	23.343615072597647
25	20.266827746643273	26.60749991421293	33.720800396091164	19.404871943052637
26	21.933221165603907	25.338504886594936	31.92994763992961	20.798326307871548
27	20.378323704302044	27.052830129072774	31.454169823540077	21.114676343085097
28	20.0576706985682	26.508477124264886	31.65194306352982	21.781909113637095
29	19.032964566944145	26.352154018021277	32.75181500930586	21.863066405728723
30	18.30587148497691	26.579122095971492	35.062891454572885	20.052114964478708
31	19.98871423428095	25.814119299040655	33.593617659826755	20.603548806851638
32	21.248340769120578	26.286465632610202	31.255797435756378	21.209396162512846
33	22.369618631535545	26.19016624172563	30.68704277533973	20.75317235139909
34	20.25424564297001	29.279753543278353	30.744669899522915	19.721330914228723
35	18.708934546371587	30.434420278211554	30.9722371252671	19.88440805014976
36	20.10097274367749	26.130523802235473	33.391922725185616	20.37658072890142
37	20.0104469588075	26.563653189290942	32.29139716363434	21.134502688267215
38	20.38371603444773	26.207160251881735	32.893322824644144	20.51580088902639
39	21.617306874240512	25.501636490497244	32.734439723280865	20.146616911981376
40	19.572197581512697	26.44534873397348	31.777328356412326	22.205125328101502
41	20.814721170233682	25.791406150851255	31.61681121561096	21.777061463304104
42	17.877426344310628	27.749965821341753	32.17140420089753	22.201203633450096
43	18.705230723645258	27.50066587107102	31.672150684580824	22.121952720702897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	249.5
2	334.0
3	798.0
4	1262.0
5	1262.0
6	2244.5
7	3227.0
8	3730.5
9	4234.0
10	6528.5
11	8823.0
12	8823.0
13	16072.5
14	23322.0
15	38318.5
16	53315.0
17	51753.5
18	50192.0
19	50192.0
20	53837.0
21	57482.0
22	46254.0
23	35026.0
24	37462.0
25	39898.0
26	39898.0
27	43012.0
28	46126.0
29	47498.0
30	48870.0
31	52040.5
32	55211.0
33	55211.0
34	59727.5
35	64244.0
36	69448.5
37	74653.0
38	77851.0
39	81049.0
40	81049.0
41	85980.5
42	90912.0
43	106609.0
44	122306.0
45	157733.5
46	193161.0
47	193161.0
48	195882.0
49	198603.0
50	179697.0
51	160791.0
52	146787.5
53	132784.0
54	132784.0
55	113584.0
56	94384.0
57	75596.0
58	56808.0
59	48679.5
60	40551.0
61	40551.0
62	35562.5
63	30574.0
64	26542.5
65	22511.0
66	19147.0
67	15783.0
68	15783.0
69	13513.5
70	11244.0
71	9419.0
72	7594.0
73	6126.5
74	4659.0
75	4659.0
76	3636.5
77	2614.0
78	2047.0
79	1480.0
80	1146.5
81	813.0
82	813.0
83	627.0
84	441.0
85	343.0
86	245.0
87	192.0
88	139.0
89	139.0
90	111.5
91	84.0
92	51.5
93	19.0
94	11.5
95	4.0
96	4.0
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1835941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.77226055324738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74993710435473	30.069516514043578
2	13.777096009731633	11.234466963517658
3	5.09198646585483	6.228353967583272
4	2.387274236850966	3.893382687877696
5	1.272491726779093	2.594118211804443
6	0.7376983717573464	1.8046578133798126
7	0.48950838646658706	1.397085443321078
8	0.3556635126316695	1.1600964325041299
9	0.2560205977924899	0.9394684668174195
>10	1.621020314940704	12.678671408205616
>50	0.15711629643249173	4.444870474936739
>100	0.08625668250953895	6.808087952919753
>500	0.007742626910479942	2.2531004969171398
>1k	0.00842180470964485	6.415260876060666
>5k	0.001222520038496833	3.4195266698086377
>10k+	5.433422393319257E-4	4.659335620302397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26175	1.425699409730487	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23570	1.2838103185233076	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20862	1.1363110252453645	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13522	0.7365160427268632	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8693	0.4734901611762034	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8262	0.45001446124902705	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7749	0.4220723868577476	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7286	0.3968537115299457	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7132	0.3884656424144349	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6039	0.32893213888681605	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5776	0.31460705981292425	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5447	0.2966870939752421	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5359	0.29189391162352163	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4688	0.2553458961916532	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4675	0.25463781243514905	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4018	0.21885234874105433	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3949	0.2150940580334553	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3261	0.17762008692000453	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3233	0.1760949834444571	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3220	0.17538689968795296	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2897	0.1577937417378881	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2773	0.15103971206046382	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2720	0.14815290905317763	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2618	0.14259717496368346	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2416	0.13159464274723426	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2329	0.12685592837678336	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2198	0.11972062283047223	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2189	0.11923041099904626	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2135	0.11628914001049052	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2111	0.11498190846002132	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2054	0.11187723352765694	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2021	0.11007979014576177	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1975	0.10757426300736243	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1853	0.10092916929247728	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1849	0.10071129736739907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.44679812695506E-5	0.0
3	0.0	0.0	0.0	5.44679812695506E-5	0.0
4	0.0	0.0	0.0	1.089359625391012E-4	0.0
5	0.0	0.0	0.0	1.089359625391012E-4	0.0
6	0.0	0.0	0.0	1.089359625391012E-4	0.0
7	0.0	0.0	0.0	1.089359625391012E-4	0.0
8	5.44679812695506E-5	0.0	0.0	1.089359625391012E-4	0.0
9	5.44679812695506E-5	0.0	0.0	1.089359625391012E-4	0.0
10	5.44679812695506E-5	0.0	0.0	2.178719250782024E-4	0.0
11	5.44679812695506E-5	0.0	0.0	3.268078876173036E-4	0.0
12	1.089359625391012E-4	0.0	0.0	4.902118314259554E-4	0.0
13	1.089359625391012E-4	0.0	0.0	5.44679812695506E-4	0.0
14	1.089359625391012E-4	0.0	0.0	8.714877003128096E-4	0.0
15	1.089359625391012E-4	0.0	0.0	0.0014161675130083156	0.0
16	1.089359625391012E-4	0.0	0.0	0.002178719250782024	0.0
17	1.089359625391012E-4	0.0	0.0	0.0032136108949034855	0.0
18	1.089359625391012E-4	0.0	0.0	0.00364935474505989	0.0
19	1.089359625391012E-4	0.0	0.0	0.004629778407911801	0.0
20	1.089359625391012E-4	0.0	0.0	0.006045945920920117	0.0
21	1.089359625391012E-4	0.0	0.0	0.00811572920916304	0.0
22	1.089359625391012E-4	0.0	0.0	0.013235719448500795	0.0
23	1.089359625391012E-4	0.0	0.0	0.022114000395437546	0.0
24	1.089359625391012E-4	0.0	0.0	0.03447823214362553	0.0
25	1.089359625391012E-4	0.0	0.0	0.041068857877241155	0.0
26	1.089359625391012E-4	0.0	0.0	0.05098203046829936	0.0
27	1.089359625391012E-4	0.0	0.0	0.12010189869935907	0.0
28	1.089359625391012E-4	0.0	0.0	0.1957034567014953	0.0
29	1.089359625391012E-4	0.0	0.0	0.2753901132988478	0.0
30	1.089359625391012E-4	0.0	0.0	0.43623406198783077	0.0
31	1.089359625391012E-4	0.0	0.0	0.6411970755051497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6250	0.0	29.452002	1
TAGGTCG	55	1.9034624E-5	23.545454	21
CGGTTAC	40	0.0019316246	23.125	31
ATTGGAC	670	0.0	22.365671	3
GTATTGG	605	0.0	22.01653	1
CGGGATA	110	7.4578566E-11	21.863636	24
AGCCGTC	125	1.8189894E-11	20.720001	12
TCACGTT	85	1.2461369E-6	19.588236	24
TTGGACC	1180	0.0	19.440678	4
ACGTTTA	90	2.1536416E-6	18.5	26
TACGTAG	50	0.0070364447	18.499998	14
TATTGGA	775	0.0	18.380646	2
TGGACCC	1215	0.0	18.271605	5
GTATCAA	10645	0.0	17.93518	2
GGACCCT	1235	0.0	17.825912	6
GACCCTC	1250	0.0	17.464	7
AGTCCGA	65	0.0015804198	17.076923	10
CGTCTGT	740	0.0	16.75	34
AAGCCGT	160	6.2937033E-10	16.1875	11
TCTATAC	185	1.8189894E-11	16.0	3
>>END_MODULE