FastQCFastQC Report
Wed 25 May 2016
SRR2088251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570684
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT179011.139694553455692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT163501.0409477654321302No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT149800.9537246193378172No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG99310.6322723093887759No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63890.40676546014347886No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT55640.3542405728969035No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA55200.35143924557708617No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT55090.35073891374713184No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA52380.3334852841182568No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA41220.2624334366428894No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT40950.26071444033300145No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG39620.25224679184355353No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT39380.25071879512365314No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32360.20602489106656718No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32050.20405122863669586No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA28820.18348693944803665No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT25360.16145832006947292No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC22320.14210369495073485No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22130.1408940308808137No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC20850.13274471504134505No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20250.12892472324159412No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG19300.12287640289198845No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG19090.12153940576207563No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18230.11606408418243262No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17050.10855143364292244No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC16290.10371277736323792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40400.029.123761
TAGGTCG551.9032343E-523.54545421
GTACGAC450.00382642320.5555553
TGCGGTC555.1442464E-420.18181812
ATTGGAC5150.020.1165073
GTATTGG5350.020.0560741
TGCGCGC759.269319E-619.73333419
CATTGCG701.2195582E-418.529
CTAGCGG609.2388707E-418.529
CGTCTGT3750.017.75999834
GTATCAA69550.017.6355152
ATCACGT953.6080892E-617.52631623
TTGCGCG852.7241444E-517.41176418
TTGGACC9850.017.2791884
TATTGGA6950.017.035972
TGCGTTA1005.8805526E-616.6537
TTGGACT2450.015.8571424
ACGTTTA1059.347381E-615.85714226
AATGCGT1059.347381E-615.85714235
TTACCGC1059.347381E-615.85714216
Produced by FastQC (version 0.11.5)