##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1570684
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.054555212888143	31.0	31.0	33.0	27.0	34.0
2	30.899096189940177	31.0	31.0	34.0	27.0	34.0
3	30.88911200470623	31.0	31.0	34.0	27.0	34.0
4	35.06624375113008	35.0	35.0	37.0	32.0	37.0
5	34.98595261682171	35.0	35.0	37.0	32.0	37.0
6	35.10306656208378	36.0	35.0	37.0	32.0	37.0
7	34.725871658462175	36.0	35.0	37.0	31.0	37.0
8	34.94947742512179	36.0	35.0	37.0	32.0	37.0
9	36.40601801508133	38.0	35.0	39.0	32.0	39.0
10	36.33022555778247	38.0	35.0	39.0	32.0	39.0
11	36.415589004535605	38.0	35.0	39.0	32.0	39.0
12	36.161078230885394	38.0	35.0	39.0	31.0	39.0
13	36.32448984009514	38.0	35.0	39.0	32.0	39.0
14	37.26709000664678	39.0	36.0	41.0	32.0	41.0
15	37.30993248801159	39.0	36.0	41.0	32.0	41.0
16	37.237861976056294	39.0	36.0	40.0	32.0	41.0
17	37.311396818201494	39.0	36.0	40.0	32.0	41.0
18	37.30583618347166	39.0	36.0	40.0	32.0	41.0
19	37.379825604641034	39.0	36.0	41.0	32.0	41.0
20	37.34020082970222	39.0	36.0	40.0	32.0	41.0
21	37.2761128272778	39.0	36.0	40.0	31.0	41.0
22	37.19334379162199	39.0	36.0	40.0	31.0	41.0
23	37.066805289924645	39.0	36.0	40.0	31.0	41.0
24	36.99755011192576	39.0	36.0	40.0	31.0	41.0
25	36.90980490028548	39.0	35.0	40.0	31.0	41.0
26	36.66208161539813	38.0	35.0	40.0	30.0	41.0
27	36.5307579373063	38.0	35.0	40.0	30.0	41.0
28	36.3635276096274	38.0	35.0	40.0	30.0	41.0
29	36.20759681769216	38.0	35.0	40.0	30.0	41.0
30	36.05443997646885	38.0	35.0	40.0	30.0	41.0
31	35.804848715591426	38.0	34.0	40.0	29.0	41.0
32	35.58510241398015	38.0	34.0	40.0	28.0	41.0
33	35.71055476467577	38.0	34.0	40.0	29.0	41.0
34	35.71055349134517	38.0	34.0	40.0	29.0	41.0
35	35.62050036799254	38.0	34.0	40.0	28.0	41.0
36	35.53739453639306	38.0	34.0	40.0	27.0	41.0
37	35.380152213939915	38.0	34.0	40.0	27.0	41.0
38	35.25201822900087	38.0	34.0	40.0	26.0	41.0
39	35.22992721642291	38.0	34.0	40.0	26.0	41.0
40	35.079860748565594	38.0	34.0	40.0	25.0	41.0
41	34.91896587728658	38.0	33.0	40.0	24.0	41.0
42	34.74284515535906	38.0	33.0	40.0	24.0	41.0
43	34.39694426122632	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	12.0
17	44.0
18	121.0
19	237.0
20	513.0
21	1085.0
22	2039.0
23	3465.0
24	5732.0
25	9162.0
26	13947.0
27	20407.0
28	28908.0
29	39193.0
30	50853.0
31	63300.0
32	78311.0
33	95693.0
34	116232.0
35	140585.0
36	172992.0
37	217214.0
38	259775.0
39	250852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.694235123041935	24.819760053581753	13.228122270297526	22.257882553078787
2	17.993307374366836	22.835146980551148	36.06568857898852	23.1058570660935
3	19.722617662114086	26.621522852464274	31.910556165339433	21.745303320082208
4	13.123199828864369	15.79649375686007	35.56565165240112	35.51465476187444
5	11.349895968889987	40.26010324164504	34.81508693028005	13.574913859184914
6	31.7438771898103	36.002149382052664	15.49280440877987	16.761169019357173
7	24.66606905017177	35.03734678649557	21.681636790086355	18.614947373246306
8	27.55385551772349	33.75440254054921	20.275561475128036	18.416180466599265
9	25.290319376781074	13.652077693539885	23.536115475805445	37.5214874538736
10	16.23865780768124	27.614020388569564	33.87218562104153	22.275136182707662
11	31.693898963763555	21.601162296171605	25.127269393461702	21.577669346603138
12	20.110792495498778	27.14524372821013	31.864206931502455	20.87975684478864
13	30.805177871551503	20.926424411275597	28.723600673337224	19.54479704383568
14	20.914964435876342	22.547628931089893	28.21993475454006	28.3174718784937
15	23.082555116115017	28.837945761209767	26.718932643357924	21.360566479317292
16	22.058797313781767	28.333133844872684	26.94991481418287	22.658154027162688
17	19.580131967983373	26.965640447091836	27.924840388009297	25.529387196915483
18	20.740836476337698	26.533535708010014	31.86267893478255	20.862948880869737
19	20.056612278472308	24.951931769853132	33.10850559374133	21.882950357933233
20	24.374349009730793	23.94370860083887	31.877958901981557	19.803983487448782
21	23.68496782293574	24.89921588301657	30.623091595763373	20.792724698284314
22	22.135260816306783	24.356522381331956	31.61546179880867	21.89275500355259
23	21.102398700184125	26.30357220166501	32.618591645423265	19.9754374527276
24	19.912152921911726	25.486666955288268	31.073150296304032	23.52802982649597
25	21.01288355900996	26.370995056930614	32.25722042116683	20.3589009628926
26	22.454739463825952	25.42440108895233	30.800148215681833	21.32071123153989
27	21.080179081215572	26.825192081920996	30.35932116199057	21.735307674872857
28	20.790560036264456	26.302489870655076	30.652123533441483	22.254826559638985
29	19.908205597051985	26.183560792622835	31.552941266352747	22.355292343972437
30	19.203098777347957	26.494826457772536	33.38812899348309	20.91394577139641
31	20.740199811037737	25.959008941327472	32.23048047856857	21.070310769066218
32	21.721237371743776	26.033053115712647	30.34569652457146	21.90001298797212
33	22.61549745206547	25.99548986301509	29.89792981911065	21.49108286580878
34	20.97971329688212	28.599769272495294	29.86641488676271	20.554102543859873
35	19.74630161127254	29.55317555918313	30.027618540712197	20.67290428883213
36	20.672776955772136	26.001983849074673	32.196164218900805	21.129074976252387
37	20.799473350463874	26.30204420494511	31.219583315294486	21.678899129296536
38	20.951445357563966	25.875924119682892	31.900560520130085	21.27207000262306
39	21.970491836677525	25.118483412322274	31.8726745799919	21.0383501710083
40	20.29065044273705	26.09270865431876	31.021453073947402	22.595187828996792
41	21.196115832338013	25.539828507834805	30.937667920472865	22.32638773935432
42	18.864074505120062	27.177458992388033	31.33386473663703	22.624601765854877
43	19.49144449169916	26.972834764981375	30.99044747383942	22.54527326948005
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87.0
1	148.0
2	209.0
3	626.0
4	1043.0
5	1043.0
6	1825.5
7	2608.0
8	2953.5
9	3299.0
10	4938.0
11	6577.0
12	6577.0
13	11836.0
14	17095.0
15	27427.5
16	37760.0
17	36682.0
18	35604.0
19	35604.0
20	38272.5
21	40941.0
22	33812.0
23	26683.0
24	29304.0
25	31925.0
26	31925.0
27	35209.5
28	38494.0
29	40366.5
30	42239.0
31	45270.0
32	48301.0
33	48301.0
34	53014.5
35	57728.0
36	63060.0
37	68392.0
38	71840.5
39	75289.0
40	75289.0
41	77851.0
42	80413.0
43	92337.0
44	104261.0
45	129648.0
46	155035.0
47	155035.0
48	157747.0
49	160459.0
50	145320.5
51	130182.0
52	122003.0
53	113824.0
54	113824.0
55	98998.5
56	84173.0
57	70086.0
58	55999.0
59	49485.5
60	42972.0
61	42972.0
62	38212.0
63	33452.0
64	29240.5
65	25029.0
66	21401.0
67	17773.0
68	17773.0
69	15131.5
70	12490.0
71	10554.5
72	8619.0
73	6875.0
74	5131.0
75	5131.0
76	3945.5
77	2760.0
78	2221.5
79	1683.0
80	1351.5
81	1020.0
82	1020.0
83	760.5
84	501.0
85	385.5
86	270.0
87	237.0
88	204.0
89	204.0
90	167.0
91	130.0
92	74.5
93	19.0
94	13.0
95	7.0
96	7.0
97	5.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1570684.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.96744347589209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70848114260185	38.54153101423675
2	12.164020072908627	11.912819307195399
3	3.894260849662781	5.720759941087252
4	1.644374623310836	3.220832856806589
5	0.8803781854997124	2.1554934517932804
6	0.49725898712986116	1.46097008070965
7	0.36192265800029294	1.240569910879178
8	0.25104217322631156	0.9834314742019616
9	0.18891876176984737	0.8325781909650473
>10	1.2133522200784261	11.385342534744233
>50	0.11907119324239696	4.004979961662576
>100	0.06414324179367017	5.898485926165485
>500	0.006453800494777642	2.3245332837217716
>1k	0.0051366983529862855	4.681959081030416
>5k	7.902612850748131E-4	2.4605535016721967
>10k+	3.951306425374066E-4	3.175159483128198
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17901	1.139694553455692	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16350	1.0409477654321302	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14980	0.9537246193378172	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9931	0.6322723093887759	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6389	0.40676546014347886	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5564	0.3542405728969035	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5520	0.35143924557708617	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5509	0.35073891374713184	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5238	0.3334852841182568	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4122	0.2624334366428894	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4095	0.26071444033300145	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3962	0.25224679184355353	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3938	0.25071879512365314	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3236	0.20602489106656718	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3205	0.20405122863669586	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2882	0.18348693944803665	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2536	0.16145832006947292	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2232	0.14210369495073485	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2213	0.1408940308808137	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2085	0.13274471504134505	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2025	0.12892472324159412	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1930	0.12287640289198845	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1909	0.12153940576207563	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1823	0.11606408418243262	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1705	0.10855143364292244	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1629	0.10371277736323792	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.273330599916979E-4	0.0
6	0.0	0.0	0.0	1.273330599916979E-4	0.0
7	0.0	0.0	0.0	1.273330599916979E-4	0.0
8	0.0	0.0	0.0	1.9099958998754682E-4	0.0
9	0.0	0.0	0.0	3.183326499792447E-4	0.0
10	0.0	0.0	0.0	3.8199917997509364E-4	0.0
11	0.0	0.0	0.0	5.093322399667916E-4	0.0
12	0.0	6.366652999584895E-5	0.0	7.003318299543383E-4	0.0
13	0.0	6.366652999584895E-5	0.0	7.639983599501873E-4	0.0
14	0.0	6.366652999584895E-5	0.0	8.913314199418852E-4	0.0
15	0.0	6.366652999584895E-5	0.0	0.0013369971299128278	0.0
16	0.0	6.366652999584895E-5	0.0	0.002610327729829807	0.0
17	0.0	6.366652999584895E-5	0.0	0.004329324039717728	0.0
18	0.0	6.366652999584895E-5	0.0	0.005029655869672066	0.0
19	0.0	6.366652999584895E-5	0.0	0.0059846538196098005	0.0
20	0.0	6.366652999584895E-5	0.0	0.0074489840095143265	0.0
21	0.0	6.366652999584895E-5	0.0	0.011268975809265263	0.0
22	0.0	1.273330599916979E-4	0.0	0.017699295338846007	0.0
23	0.0	1.273330599916979E-4	0.0	0.03068726745799919	0.0
24	0.0	1.273330599916979E-4	0.0	0.05118789011666255	0.0
25	0.0	1.273330599916979E-4	0.0	0.05831854147619763	0.0
26	0.0	1.273330599916979E-4	0.0	0.06971485034545459	0.0
27	0.0	1.273330599916979E-4	0.0	0.14337702555065182	0.0
28	0.0	1.273330599916979E-4	0.0	0.21277354324612716	0.0
29	0.0	1.273330599916979E-4	0.0	0.29808669344056477	0.0
30	0.0	1.273330599916979E-4	0.0	0.4521596960305192	0.0
31	0.0	1.273330599916979E-4	0.0	0.6635962421467335	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4040	0.0	29.12376	1
TAGGTCG	55	1.9032343E-5	23.545454	21
GTACGAC	45	0.003826423	20.555555	3
TGCGGTC	55	5.1442464E-4	20.181818	12
ATTGGAC	515	0.0	20.116507	3
GTATTGG	535	0.0	20.056074	1
TGCGCGC	75	9.269319E-6	19.733334	19
CATTGCG	70	1.2195582E-4	18.5	29
CTAGCGG	60	9.2388707E-4	18.5	29
CGTCTGT	375	0.0	17.759998	34
GTATCAA	6955	0.0	17.635515	2
ATCACGT	95	3.6080892E-6	17.526316	23
TTGCGCG	85	2.7241444E-5	17.411764	18
TTGGACC	985	0.0	17.279188	4
TATTGGA	695	0.0	17.03597	2
TGCGTTA	100	5.8805526E-6	16.65	37
TTGGACT	245	0.0	15.857142	4
ACGTTTA	105	9.347381E-6	15.857142	26
AATGCGT	105	9.347381E-6	15.857142	35
TTACCGC	105	9.347381E-6	15.857142	16
>>END_MODULE