FastQCFastQC Report
Wed 25 May 2016
SRR2088250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351678
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT176471.3055624194519702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT156831.1602615415801691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT147221.089164727102165No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG106070.7847283154715843No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT61550.4553599303976243No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60990.4512169318432348No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT60310.4461861478843334No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58640.4338311343382078No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA56360.41696321165247935No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA43720.3234498157105465No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT43290.3202685846777117No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT41440.30658189302481803No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG39890.2951146648832044No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33960.251243269476902No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA33490.24776610997589663No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA30440.22520156427788277No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT28180.20848160582623967No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC23880.17666929549789226No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA23450.17348806446505752No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC22360.16542401370740664No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.15906155164173716No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG20330.15040564394774497No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG19730.14596671692518484No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18370.13590514900738193No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18280.13523930995399794No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC17120.12665738437704838No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA16590.12273633217378696No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG15710.11622590587403213No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA14870.11001140804244797No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC14860.10993742592540531No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA14470.10705212336074123No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14130.1045367313812905No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC14130.1045367313812905No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC13670.10113355399732776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39650.030.0945781
TAGAACG400.001931362723.1254
TATCACG502.7024435E-422.27
GCGGTAT603.7269107E-521.58333411
GTATTGG5650.021.2831861
TATTGGA6700.019.6044772
TTGGACC9000.019.3222224
ATTGGAC6100.019.1065563
GGACCCT9300.019.0967736
CGTCTGT4100.018.95122134
GAACGCC500.00703550818.535
TGGACCC9650.017.6373045
GTATCAA69850.017.559772
AAGCCGT852.7236902E-517.41176437
GACGGAC752.0676052E-417.2666667
GACCCTC10100.017.2178237
TAGACAG1401.869921E-917.1785725
TGCGGTA650.001580128617.07692310
CGACGAC904.446887E-516.44444525
ACGCATA803.3831177E-416.18757
Produced by FastQC (version 0.11.5)