FastQCFastQC Report
Wed 25 May 2016
SRR2088249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413831
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT163981.1598274475520765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT145081.0261481039813103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133650.9453039295361327No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG97540.6898985805234148No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT55490.39247972353131316No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA54200.38335557785902274No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53990.3818702518193476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.3711900502959689No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT52130.36871450689651025No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA40880.28914346905676847No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT39950.2825655965953498No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT39430.2788876464018684No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG38140.269763500729578No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA31460.22251598670562467No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA29000.2051164530980011No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA28850.20405550592680458No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT25900.18319021155993892No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA23010.16274929606155192No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC22950.1623249171930733No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC20060.14188400169468626No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG18690.13219401753109106No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18630.13176963866261243No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG18400.13014285300011103No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18260.12915263564032758No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17680.12505030657836758No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC15810.11182383184411715No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG15050.10644836617672127No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA14630.1034777140973709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.030.591341
TATACCG851.9772415E-923.9411775
ATTGGAC3900.023.7179493
CGCAATA400.001931406123.12536
GTATTGG4000.022.21
TAGGTCG450.003826236820.55555521
TTGGACC8000.018.9625024
GTATCAA62400.018.7075332
ACTGACG500.00703566218.523
ATAGACG500.00703566218.53
TGCGCGC500.00703566218.519
GGACCCT7850.017.9108286
CGTCTGT3750.017.26666634
TGTACGT752.0676902E-417.2666665
TATTGGA5800.017.2241382
TGGACCC8550.017.0935675
ATGTCCG1301.3938916E-817.0769237
TAGTACC650.001580176817.0769234
GTACTAG1554.0199666E-1016.7096771
ACCGACC1352.2204404E-816.4444458
Produced by FastQC (version 0.11.5)