##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088249_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1413831
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.041177481608482	31.0	31.0	33.0	27.0	34.0
2	30.887577086653213	31.0	31.0	34.0	27.0	34.0
3	30.89935289295538	31.0	31.0	34.0	27.0	34.0
4	35.07967005957572	35.0	35.0	37.0	32.0	37.0
5	34.99613532310439	35.0	35.0	37.0	32.0	37.0
6	35.099004760823604	36.0	35.0	37.0	32.0	37.0
7	34.7209355290696	35.0	35.0	37.0	31.0	37.0
8	34.94050420453364	36.0	35.0	37.0	32.0	37.0
9	36.397237717945075	38.0	35.0	39.0	32.0	39.0
10	36.33249447776998	38.0	35.0	39.0	32.0	39.0
11	36.41098830058189	38.0	35.0	39.0	32.0	39.0
12	36.15706403382017	38.0	35.0	39.0	31.0	39.0
13	36.325646417428956	38.0	35.0	39.0	32.0	39.0
14	37.26562085567511	39.0	36.0	40.0	32.0	41.0
15	37.30110388016672	39.0	36.0	41.0	32.0	41.0
16	37.221604986734626	39.0	36.0	40.0	32.0	41.0
17	37.306952528272475	39.0	36.0	40.0	32.0	41.0
18	37.30912817727154	39.0	36.0	40.0	32.0	41.0
19	37.37977452750718	39.0	36.0	40.0	32.0	41.0
20	37.34131589984942	39.0	36.0	40.0	32.0	41.0
21	37.29107368561023	39.0	36.0	40.0	31.0	41.0
22	37.21190934418612	39.0	36.0	40.0	31.0	41.0
23	37.0948352384408	39.0	36.0	40.0	31.0	41.0
24	37.02736182754516	39.0	36.0	40.0	31.0	41.0
25	36.94096748479839	39.0	36.0	40.0	31.0	41.0
26	36.70803441146785	38.0	35.0	40.0	30.0	41.0
27	36.56725662402366	38.0	35.0	40.0	30.0	41.0
28	36.401111589716166	38.0	35.0	40.0	30.0	41.0
29	36.24078125320494	38.0	35.0	40.0	30.0	41.0
30	36.08891303133119	38.0	35.0	40.0	30.0	41.0
31	35.857351409043936	38.0	34.0	40.0	29.0	41.0
32	35.6633699501567	38.0	34.0	40.0	29.0	41.0
33	35.7825305853387	38.0	34.0	40.0	29.0	41.0
34	35.78216844870426	38.0	34.0	40.0	29.0	41.0
35	35.67443421455605	38.0	34.0	40.0	28.0	41.0
36	35.596057803231076	38.0	34.0	40.0	27.0	41.0
37	35.44635745007713	38.0	34.0	40.0	27.0	41.0
38	35.32334769855803	38.0	34.0	40.0	27.0	41.0
39	35.29674055810065	38.0	34.0	40.0	26.0	41.0
40	35.14140869736199	38.0	34.0	40.0	25.0	41.0
41	34.9966813572485	38.0	34.0	40.0	25.0	41.0
42	34.81326481029203	38.0	33.0	40.0	24.0	41.0
43	34.485104655365454	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	7.0
16	11.0
17	27.0
18	102.0
19	209.0
20	441.0
21	950.0
22	1688.0
23	2929.0
24	5059.0
25	8079.0
26	12364.0
27	17998.0
28	25745.0
29	34524.0
30	45378.0
31	56405.0
32	70309.0
33	86244.0
34	105158.0
35	126651.0
36	155198.0
37	195211.0
38	232586.0
39	230556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.44177203640322	24.820081042217918	13.370622089910322	22.36752483146854
2	17.93524119926639	22.801310764865107	36.25242338016354	23.011024655704958
3	19.730929651422272	26.35944465781271	32.058004103743656	21.851621587021363
4	13.067757037439412	15.812851748193385	35.44115244325524	35.678238771111964
5	11.43156431002008	40.10422745009835	34.96047264489179	13.503735594989783
6	31.816956906447803	35.835824790940364	15.404104168037055	16.94311413457478
7	24.634981125749825	34.96832365395864	21.433042563078615	18.963652657212922
8	27.491475289479432	33.79336002676416	20.301365580468953	18.413799103287452
9	25.182217676653007	13.718400572628553	23.529969282042902	37.56941246867554
10	16.27330282049269	27.62239617040509	33.945570580925164	22.15873042817706
11	31.623298682798723	21.546493180585234	25.22628234916337	21.603925787452674
12	20.177800599930258	27.087537336499203	31.742336955406973	20.992325108163566
13	31.151389381050492	20.86161641667215	28.604833250933105	19.382160951344254
14	21.02889242066414	22.42382576135337	27.958999342920055	28.58828247506244
15	23.13883342492844	28.861582466362666	26.564207461853645	21.435376646855246
16	22.114312106609628	28.362371457408987	26.8009401406533	22.722376295328083
17	19.60538423616401	27.062569713070374	27.546078703890352	25.78596734687526
18	20.84322666570474	26.570290225635173	31.854160787251097	20.732322321408994
19	20.02990456426546	24.842502392435872	33.060882099770055	22.066710943528612
20	24.54190069393018	23.897198462899738	31.769355743366784	19.7915450998033
21	23.756658327621903	24.78888919538474	30.422518674438457	21.031933802554903
22	22.2042804267271	24.316201865710966	31.394770662122983	22.084747045438952
23	21.19638061409037	26.486546128922058	32.48599019260435	19.831083064383225
24	19.90499571730992	25.440169298876597	30.822071379111083	23.8327636047024
25	21.05124304107068	26.415887047320364	32.069108684135514	20.46376122747344
26	22.524403553182808	25.352181413478696	30.64864188152615	21.474773151812347
27	21.179829838219703	26.833475853903334	30.17652039034368	21.810173917533284
28	20.87618675782325	26.326767484939857	30.389346392885713	22.407699364351185
29	19.968086709090407	26.187217567021804	31.38394900097678	22.46074672291101
30	19.299902180670816	26.539239838424816	33.29846353630667	20.862394444597694
31	20.68189196587145	25.882867188511216	32.12979486232796	21.305445983289374
32	21.94406545053829	26.117619432591304	30.032302304872367	21.90601281199804
33	22.853863014745045	25.893123011166114	29.63734703794159	21.61566693614725
34	20.949109193390157	28.898149778863246	29.707864659920457	20.44487636782614
35	19.77308461902448	29.897279094884748	29.808513181561302	20.521123104529465
36	20.781479540341103	26.10969769371304	32.074554879614325	21.034267886331534
37	20.897052052190112	26.45294946850083	31.06757455452597	21.58242392478309
38	21.10365383132779	26.026448705679815	31.69480652213737	21.175090940855025
39	22.09111272846613	25.480697480816307	31.629239986957426	20.798949803760138
40	20.267627460424904	26.33532579212084	30.786352824347464	22.61069392310679
41	21.463668571420488	25.811076429926917	30.558036993105965	22.167218005546633
42	18.838248701577488	27.52167691895283	31.18364217505487	22.456432204414813
43	19.614154732779234	27.271151926927615	30.654653915496265	22.460039424796882
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	175.0
2	203.0
3	519.5
4	836.0
5	836.0
6	1484.5
7	2133.0
8	2311.0
9	2489.0
10	3946.0
11	5403.0
12	5403.0
13	9777.5
14	14152.0
15	23448.0
16	32744.0
17	32086.0
18	31428.0
19	31428.0
20	34059.0
21	36690.0
22	30142.0
23	23594.0
24	26099.0
25	28604.0
26	28604.0
27	31300.5
28	33997.0
29	35985.0
30	37973.0
31	41339.0
32	44705.0
33	44705.0
34	48700.5
35	52696.0
36	56881.0
37	61066.0
38	63488.0
39	65910.0
40	65910.0
41	69643.5
42	73377.0
43	83422.0
44	93467.0
45	117123.0
46	140779.0
47	140779.0
48	144102.0
49	147425.0
50	133843.5
51	120262.0
52	112143.0
53	104024.0
54	104024.0
55	90522.5
56	77021.0
57	63320.0
58	49619.0
59	43377.0
60	37135.0
61	37135.0
62	32962.5
63	28790.0
64	25231.5
65	21673.0
66	18695.5
67	15718.0
68	15718.0
69	13381.5
70	11045.0
71	9512.5
72	7980.0
73	6334.0
74	4688.0
75	4688.0
76	3770.0
77	2852.0
78	2155.5
79	1459.0
80	1156.5
81	854.0
82	854.0
83	637.5
84	421.0
85	326.0
86	231.0
87	183.0
88	135.0
89	135.0
90	108.0
91	81.0
92	47.0
93	13.0
94	10.5
95	8.0
96	8.0
97	5.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1413831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.77089193888884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.09742756197198	36.83012880829274
2	12.803222473243068	12.23242714477695
3	4.225161830441582	6.0551924767902765
4	1.8270892982117044	3.4912674171028626
5	0.9800714803290731	2.3409444389593483
6	0.5980700737466239	1.7142204518899948
7	0.4113645636823521	1.3755876483410459
8	0.3042596538388416	1.1627804035919223
9	0.21845763508356883	0.939232447092214
>10	1.3590049291031134	12.077954535466892
>50	0.10526956194709604	3.4206896884782747
>100	0.056180340739255215	5.0163780311394035
>500	0.007660942507794044	2.652637081489747
>1k	0.005407724123148737	4.888570610195559
>5k	9.012873538581228E-4	2.625153447820684
>10k+	4.506436769290614E-4	3.176835368572001
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16398	1.1598274475520765	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14508	1.0261481039813103	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13365	0.9453039295361327	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9754	0.6898985805234148	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5549	0.39247972353131316	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5420	0.38335557785902274	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5399	0.3818702518193476	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5248	0.3711900502959689	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5213	0.36871450689651025	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4088	0.28914346905676847	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3995	0.2825655965953498	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3943	0.2788876464018684	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3814	0.269763500729578	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3146	0.22251598670562467	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2900	0.2051164530980011	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2885	0.20405550592680458	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2590	0.18319021155993892	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2301	0.16274929606155192	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2295	0.1623249171930733	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2006	0.14188400169468626	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1869	0.13219401753109106	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1863	0.13176963866261243	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1840	0.13014285300011103	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1826	0.12915263564032758	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1768	0.12505030657836758	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1581	0.11182383184411715	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1505	0.10644836617672127	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1463	0.1034777140973709	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.072981141310383E-5	0.0
3	0.0	0.0	0.0	7.072981141310383E-5	0.0
4	0.0	0.0	0.0	1.4145962282620766E-4	0.0
5	0.0	0.0	0.0	1.4145962282620766E-4	0.0
6	0.0	0.0	0.0	1.4145962282620766E-4	0.0
7	0.0	0.0	0.0	1.4145962282620766E-4	0.0
8	0.0	0.0	0.0	1.4145962282620766E-4	0.0
9	0.0	0.0	0.0	2.121894342393115E-4	0.0
10	0.0	0.0	0.0	2.121894342393115E-4	0.0
11	0.0	0.0	0.0	2.829192456524153E-4	0.0
12	0.0	0.0	0.0	4.24378868478623E-4	0.0
13	7.072981141310383E-5	0.0	0.0	4.951086798917268E-4	0.0
14	1.4145962282620766E-4	0.0	0.0	7.072981141310383E-4	0.0
15	1.4145962282620766E-4	0.0	0.0	0.0012024067940227651	0.0
16	1.4145962282620766E-4	0.0	0.0	0.0021218943423931148	0.0
17	1.4145962282620766E-4	0.0	0.0	0.003041381890763465	0.0
18	1.4145962282620766E-4	0.0	0.0	0.0038194098163076068	0.0
19	1.4145962282620766E-4	0.0	0.0	0.004526707930438645	0.0
20	1.4145962282620766E-4	0.0	0.0	0.005587655101635202	0.0
21	1.4145962282620766E-4	0.0	0.0	0.007921738878267628	0.0
22	1.4145962282620766E-4	0.0	0.0	0.01365085360272904	0.0
23	1.4145962282620766E-4	0.0	0.0	0.022421350217953915	0.0
24	1.4145962282620766E-4	0.0	0.0	0.0369209615576402	0.0
25	1.4145962282620766E-4	0.0	0.0	0.044984160058734034	0.0
26	1.4145962282620766E-4	0.0	0.0	0.05474487403374236	0.0
27	1.4145962282620766E-4	0.0	0.0	0.11309696844955303	0.0
28	1.4145962282620766E-4	0.0	0.0	0.18262437306863408	0.0
29	1.4145962282620766E-4	0.0	0.0	0.2566784856181538	0.0
30	1.4145962282620766E-4	0.0	0.0	0.4044330616601277	0.0
31	1.4145962282620766E-4	0.0	0.0	0.5983742045548583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3695	0.0	30.59134	1
TATACCG	85	1.9772415E-9	23.941177	5
ATTGGAC	390	0.0	23.717949	3
CGCAATA	40	0.0019314061	23.125	36
GTATTGG	400	0.0	22.2	1
TAGGTCG	45	0.0038262368	20.555555	21
TTGGACC	800	0.0	18.962502	4
GTATCAA	6240	0.0	18.707533	2
ACTGACG	50	0.007035662	18.5	23
ATAGACG	50	0.007035662	18.5	3
TGCGCGC	50	0.007035662	18.5	19
GGACCCT	785	0.0	17.910828	6
CGTCTGT	375	0.0	17.266666	34
TGTACGT	75	2.0676902E-4	17.266666	5
TATTGGA	580	0.0	17.224138	2
TGGACCC	855	0.0	17.093567	5
ATGTCCG	130	1.3938916E-8	17.076923	7
TAGTACC	65	0.0015801768	17.076923	4
GTACTAG	155	4.0199666E-10	16.709677	1
ACCGACC	135	2.2204404E-8	16.444445	8
>>END_MODULE