FastQCFastQC Report
Wed 25 May 2016
SRR2088248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871453
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128051.4693850385505587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT111941.2845213683354122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104691.2013269791945176No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG74270.8522547974474813No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT42750.49056001872734384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42690.489871513437902No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41720.4787406779252582No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA40940.4697901091625136No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT40480.4645115686101258No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA30910.354694974944145No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT28860.33117104422154725No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT28430.3262367563138804No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG28250.32417124044555473No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA25750.2954835200521428No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA23980.27517261401360715No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21970.25210768681730394No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT19180.22009219085825626No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17410.19978128481972063No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC15850.18188014729423158No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC15490.17774911555758027No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15180.1741918382287972No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14710.16879854679483575No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14510.16650352916336278No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14310.16420851153188984No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT13450.15433993571655613No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC11760.13494703673060968No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA11410.13093075587553202No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG11220.1287504891256327No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10190.11693114832354701No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10140.11635739391567874No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA9910.11371812363948487No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC9820.11268536570532203No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA9660.11084935160014368No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC9590.11004609542912813No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC9510.10912808837653895No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG9050.10384954782415116No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC9000.10327579341628292No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG8860.10166928107425185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.029.5758551
GTATTGG2500.025.161
ATTGGAC2750.023.5454543
CTTACTC551.9019744E-523.5454543
GTTCGCT450.003825076620.55555537
TATTGGA3400.019.0441172
TAGAGTG609.23437E-418.55
AACCCGT609.23437E-418.529
TAAGGTG801.6159393E-518.55
GTATTAG500.00703355318.4999981
GTTAGTG500.00703355318.4999982
GTATCAA51150.018.3734112
CGTCTGT2400.017.72916834
ATACCGT1054.793965E-717.6190476
AGTATCA1800.017.4722211
TATAGTG852.7218926E-517.4117645
TACACTT1401.8662831E-917.1785725
TTTTTAC6900.017.159421
CTACGGT650.001579519617.0769236
TCTATAG650.001579519617.0769233
Produced by FastQC (version 0.11.5)