##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	871453
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.974155806451982	31.0	30.0	33.0	27.0	34.0
2	30.833478110695584	31.0	31.0	34.0	27.0	34.0
3	30.831307024016212	31.0	31.0	34.0	27.0	34.0
4	35.027284317111764	35.0	35.0	37.0	32.0	37.0
5	34.957376932548286	35.0	35.0	37.0	32.0	37.0
6	35.08351224908285	36.0	35.0	37.0	32.0	37.0
7	34.66355041522606	35.0	35.0	37.0	31.0	37.0
8	34.9130429294523	36.0	35.0	37.0	32.0	37.0
9	36.31386775878906	38.0	35.0	39.0	32.0	39.0
10	36.281128184767276	38.0	35.0	39.0	32.0	39.0
11	36.376730586732734	38.0	35.0	39.0	32.0	39.0
12	36.11037887298569	38.0	35.0	39.0	31.0	39.0
13	36.25803112732414	38.0	35.0	39.0	32.0	39.0
14	37.15267031038966	39.0	36.0	40.0	31.0	41.0
15	37.19219739905652	39.0	36.0	40.0	32.0	41.0
16	37.10621341598457	39.0	36.0	40.0	31.0	41.0
17	37.23238660031006	39.0	36.0	40.0	32.0	41.0
18	37.24502755742421	39.0	36.0	40.0	32.0	41.0
19	37.34216532618512	39.0	36.0	40.0	32.0	41.0
20	37.31901663084527	39.0	36.0	40.0	31.0	41.0
21	37.24586179633325	39.0	36.0	40.0	31.0	41.0
22	37.16618222669496	39.0	36.0	40.0	31.0	41.0
23	37.01895799314478	39.0	36.0	40.0	31.0	41.0
24	36.95401587922699	39.0	35.0	40.0	31.0	41.0
25	36.86397086245615	39.0	35.0	40.0	31.0	41.0
26	36.61428556674887	38.0	35.0	40.0	30.0	41.0
27	36.47321886550393	38.0	35.0	40.0	30.0	41.0
28	36.30009880050903	38.0	35.0	40.0	30.0	41.0
29	36.1357193101636	38.0	35.0	40.0	30.0	41.0
30	35.9510197337091	38.0	34.0	40.0	29.0	41.0
31	35.665981986406614	38.0	34.0	40.0	29.0	41.0
32	35.450050662514215	38.0	34.0	40.0	27.0	41.0
33	35.53611841372971	38.0	34.0	40.0	28.0	41.0
34	35.4986361857725	38.0	34.0	40.0	28.0	41.0
35	35.39621643393276	38.0	34.0	40.0	27.0	41.0
36	35.30798562859959	38.0	34.0	40.0	27.0	41.0
37	35.12787149737278	38.0	34.0	40.0	26.0	41.0
38	34.98994093772125	38.0	33.0	40.0	25.0	41.0
39	34.93165437493474	38.0	33.0	40.0	25.0	41.0
40	34.74920850579434	38.0	33.0	40.0	24.0	41.0
41	34.57347900575246	38.0	33.0	40.0	23.0	41.0
42	34.324809255347105	38.0	33.0	40.0	22.0	41.0
43	33.981685759300845	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	3.0
16	6.0
17	28.0
18	53.0
19	148.0
20	328.0
21	637.0
22	1177.0
23	2074.0
24	3504.0
25	5486.0
26	8204.0
27	12169.0
28	16978.0
29	22922.0
30	29869.0
31	36389.0
32	44351.0
33	54350.0
34	65984.0
35	79098.0
36	96686.0
37	122477.0
38	139485.0
39	129044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.63897077639299	25.13813137369428	13.668780760408191	21.55411708950454
2	18.043543369521934	22.640119432717544	36.33758791351915	22.978749284241378
3	20.048126519731987	26.607286910481694	32.25280078214201	21.091785787644312
4	12.779002424686128	15.56194080461023	34.75196023193448	36.90709653876916
5	10.900530493325515	40.748267548565444	34.633078318624186	13.718123639484862
6	31.872057357080646	35.16253888620499	15.103855285368232	17.86154847134613
7	24.122586071767497	35.79665225778097	20.991837769793666	19.088923900657868
8	28.196930872921435	33.31252517347465	19.937391919013418	18.553152034590507
9	24.887630199219004	13.602110498213902	24.065210630980673	37.44504867158642
10	16.542831340301774	27.800925580610773	33.322852752816274	22.333390326271182
11	30.830004601510353	21.27102666466235	25.524038588426457	22.374930145400842
12	20.333970965731943	27.32838145029049	31.70991436141708	20.627733222560483
13	31.7019965505885	20.44642682967412	29.374504419630203	18.477072200107177
14	21.014443693463676	22.649643755888153	28.280584265588622	28.05532828505955
15	23.187595888705413	28.4796770451189	27.545490118227832	20.78723694794785
16	21.93474576368433	28.195094858816255	27.3636099709336	22.506549406565814
17	18.9990739603857	26.853083298812443	27.74573040657385	26.402112334228008
18	20.356232636757234	26.07094129000646	32.977682101042745	20.595143972193565
19	19.36685053582924	24.21117375234235	34.506393345366874	21.915582366461532
20	24.911613133467895	22.715396011029853	33.079351382117	19.293639473385255
21	24.052473283125998	24.043293212600105	31.494985960229638	20.409247544044256
22	21.865206729450698	23.48066964024451	32.69447692531898	21.95964670498581
23	20.730550012450472	26.069105275901283	34.0312099447704	19.169134766877846
24	19.13941428855027	24.89807252944221	31.989677010693633	23.972836171313887
25	20.51160533040795	26.138873811898062	33.50186412807116	19.847656729622827
26	22.405912883425728	24.78309214610541	31.706471834969875	21.10452313549899
27	20.80605609252593	26.568271610746653	31.067309424604655	21.558362872122768
28	20.263743426208872	25.814128817044633	31.483510872072273	22.438616884674218
29	19.30419655449003	25.869209240199986	32.655232123820795	22.17136208148919
30	18.5157432471975	25.915339094592593	35.09093433610303	20.477983322106873
31	20.234137698762872	25.27020963838555	33.55625604593707	20.93939661691451
32	21.73771850002238	25.671608222130164	30.844348461707057	21.7463248161404
33	22.91655430642846	25.48353152723096	30.435261568897005	21.16465259744358
34	20.55050587926142	29.127216269838996	30.458670748738026	19.863607102161563
35	18.94938682866431	30.43239279685766	30.573995384719545	20.044224989758483
36	20.233678695236577	25.837767498648805	33.39112952735259	20.53742427876202
37	20.354740875296777	26.40050582188598	32.039364142415025	21.205389160402223
38	20.807088850460094	25.74631104603461	32.77640905476256	20.67019104874273
39	22.038365809745333	25.143754166891384	32.484482812039204	20.333397211324076
40	19.838820911741653	26.221379695749512	31.31379431822485	22.626005074283984
41	21.373384451026045	25.634428936500303	31.116193300155025	21.875993312318624
42	18.28520872611604	27.722321226732827	31.845435152555556	22.147034894595578
43	19.175446065364397	27.537113303872957	31.116193300155025	22.171247330607617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	98.0
2	131.0
3	339.0
4	547.0
5	547.0
6	988.5
7	1430.0
8	1629.0
9	1828.0
10	2918.0
11	4008.0
12	4008.0
13	7380.0
14	10752.0
15	17836.0
16	24920.0
17	24007.0
18	23094.0
19	23094.0
20	24679.5
21	26265.0
22	20672.0
23	15079.0
24	16141.0
25	17203.0
26	17203.0
27	18610.5
28	20018.0
29	20718.5
30	21419.0
31	22908.0
32	24397.0
33	24397.0
34	26491.0
35	28585.0
36	30888.5
37	33192.0
38	35070.5
39	36949.0
40	36949.0
41	39271.0
42	41593.0
43	49062.0
44	56531.0
45	74894.5
46	93258.0
47	93258.0
48	94833.0
49	96408.0
50	87458.5
51	78509.0
52	72849.0
53	67189.0
54	67189.0
55	57534.0
56	47879.0
57	38186.0
58	28493.0
59	24530.5
60	20568.0
61	20568.0
62	18044.5
63	15521.0
64	13927.0
65	12333.0
66	10465.5
67	8598.0
68	8598.0
69	7124.5
70	5651.0
71	4733.5
72	3816.0
73	3043.0
74	2270.0
75	2270.0
76	1765.0
77	1260.0
78	1018.0
79	776.0
80	608.5
81	441.0
82	441.0
83	324.5
84	208.0
85	171.0
86	134.0
87	105.5
88	77.0
89	77.0
90	61.0
91	45.0
92	27.0
93	9.0
94	6.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	871453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.89343529755481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75231878968503	40.29010592660671
2	11.16055382794837	11.136767405992394
3	3.3453528296066617	5.007334348544156
4	1.4345724855928395	2.863029979583149
5	0.7659983420258942	1.9109144357951604
6	0.4748134382673343	1.4214044136360466
7	0.33417448468929006	1.167117912095717
8	0.24405148375609026	0.974125353124539
9	0.1865401092047566	0.8376414182105699
>10	1.1437775944640607	10.725497388636622
>50	0.08472678425145264	2.8950663358546853
>100	0.05571034705595884	5.375824688602722
>500	0.010445690071367334	3.692093742240679
>1k	0.006035287596790015	6.850971273518777
>5k	2.3212644603038524E-4	0.8601643785445441
>10k+	6.963793380911556E-4	3.991940999013511
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12805	1.4693850385505587	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11194	1.2845213683354122	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10469	1.2013269791945176	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7427	0.8522547974474813	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4275	0.49056001872734384	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4269	0.489871513437902	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4172	0.4787406779252582	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4094	0.4697901091625136	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4048	0.4645115686101258	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3091	0.354694974944145	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2886	0.33117104422154725	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2843	0.3262367563138804	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2825	0.32417124044555473	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2575	0.2954835200521428	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2398	0.27517261401360715	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2197	0.25210768681730394	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1918	0.22009219085825626	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1741	0.19978128481972063	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1585	0.18188014729423158	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1549	0.17774911555758027	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1518	0.1741918382287972	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1471	0.16879854679483575	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1451	0.16650352916336278	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1431	0.16420851153188984	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1345	0.15433993571655613	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1176	0.13494703673060968	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1141	0.13093075587553202	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1122	0.1287504891256327	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1019	0.11693114832354701	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1014	0.11635739391567874	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	991	0.11371812363948487	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	982	0.11268536570532203	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	966	0.11084935160014368	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	959	0.11004609542912813	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	951	0.10912808837653895	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	905	0.10384954782415116	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	900	0.10327579341628292	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	886	0.10166928107425185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.2950176314729537E-4	0.0
10	0.0	0.0	0.0	3.4425264472094305E-4	0.0
11	0.0	0.0	0.0	9.180070525891815E-4	0.0
12	0.0	0.0	0.0	0.0011475088157364768	0.0
13	0.0	0.0	0.0	0.0011475088157364768	0.0
14	0.0	0.0	0.0	0.0013770105788837722	0.0
15	0.0	0.0	0.0	0.0017212632236047153	0.0
16	0.0	0.0	0.0	0.0030982738024884875	0.0
17	0.0	0.0	0.0	0.006081796723403327	0.0
18	0.0	0.0	0.0	0.006770302012845213	0.0
19	0.0	0.0	0.0	0.008147312591728986	0.0
20	0.0	0.0	0.0	0.010098077578480996	0.0
21	0.0	0.0	0.0	0.01388485667041137	0.0
22	0.0	0.0	0.0	0.02122891309112482	0.0
23	0.0	0.0	0.0	0.03568752416940443	0.0
24	0.0	0.0	0.0	0.059326205773575856	0.0
25	0.0	0.0	0.0	0.0693095324704832	0.0
26	0.0	0.0	0.0	0.07917810828581691	0.0
27	0.0	0.0	0.0	0.137127303480509	0.0
28	0.0	0.0	0.0	0.20494507449053478	0.0
29	0.0	0.0	0.0	0.28435268453949897	0.0
30	0.0	0.0	0.0	0.4384631184929078	0.0
31	0.0	0.0	0.0	0.6228677851817597	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3065	0.0	29.575855	1
GTATTGG	250	0.0	25.16	1
ATTGGAC	275	0.0	23.545454	3
CTTACTC	55	1.9019744E-5	23.545454	3
GTTCGCT	45	0.0038250766	20.555555	37
TATTGGA	340	0.0	19.044117	2
TAGAGTG	60	9.23437E-4	18.5	5
AACCCGT	60	9.23437E-4	18.5	29
TAAGGTG	80	1.6159393E-5	18.5	5
GTATTAG	50	0.007033553	18.499998	1
GTTAGTG	50	0.007033553	18.499998	2
GTATCAA	5115	0.0	18.373411	2
CGTCTGT	240	0.0	17.729168	34
ATACCGT	105	4.793965E-7	17.619047	6
AGTATCA	180	0.0	17.472221	1
TATAGTG	85	2.7218926E-5	17.411764	5
TACACTT	140	1.8662831E-9	17.178572	5
TTTTTAC	690	0.0	17.15942	1
CTACGGT	65	0.0015795196	17.076923	6
TCTATAG	65	0.0015795196	17.076923	3
>>END_MODULE