FastQCFastQC Report
Wed 25 May 2016
SRR2088247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612038
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT169241.0498511821681622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT149970.9303130571363702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139290.8640615171602655No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG100680.624551034156763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58630.36370110382013326No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58280.3615299391205418No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT55960.34713821882610707No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA55570.3447189210179909No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT52870.32796993619257114No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA39550.2453416110538337No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG38720.24019284905194543No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT38590.23938641644924005No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT37260.23113599059079254No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33330.2067569126782371No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA31700.19664548850585406No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA30720.19056622734699802No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT24150.14981036427180996No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA22740.141063672196313No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC21810.13529457742311285No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC20920.12977361575843746No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20760.1287810833243385No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG19150.11879372570621784No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG19020.11798729310351244No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18340.11376903025859192No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16460.10210677415792928No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC16380.10161050794087981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39200.028.4579071
ATTGGAC5900.025.0847453
GTATTGG5950.024.2521021
TATTGGA7300.020.0205462
TTGGACC10250.019.4926824
TATACCG1052.2611857E-819.3809535
TGGACCC11050.019.2533935
TAGACCG500.0070360818.55
GGACCCT11600.018.3405176
CGACGAG953.6082092E-617.52631624
TGCGACG852.7242162E-517.41176422
TTACGCT650.00158030717.0769234
GTATCAA67900.016.9197332
GACCCTC12050.016.5809147
ACGGACC1251.6588092E-716.2799998
GTATACG1151.2428773E-616.0869561
CGTCTGT3000.016.03333334
GCGCGAT1302.5906593E-715.65384613
CGACGAA1601.0972144E-815.0312510
ACCCTCG14050.014.8790048
Produced by FastQC (version 0.11.5)