##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1612038
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.98104201017594	31.0	30.0	33.0	27.0	34.0
2	30.848971302165335	31.0	31.0	34.0	27.0	34.0
3	30.821266620265774	31.0	31.0	34.0	27.0	34.0
4	35.026384613762204	35.0	35.0	37.0	32.0	37.0
5	34.94233882824102	35.0	35.0	37.0	32.0	37.0
6	35.06353014010836	36.0	35.0	37.0	32.0	37.0
7	34.664324290122195	35.0	35.0	37.0	31.0	37.0
8	34.893088748528264	36.0	35.0	37.0	32.0	37.0
9	36.331633621539936	38.0	35.0	39.0	32.0	39.0
10	36.276959972407596	38.0	35.0	39.0	32.0	39.0
11	36.36999127812123	38.0	35.0	39.0	32.0	39.0
12	36.095487823488035	38.0	35.0	39.0	31.0	39.0
13	36.26128602427486	38.0	35.0	39.0	32.0	39.0
14	37.194233014358225	39.0	36.0	40.0	32.0	41.0
15	37.24699107589275	39.0	36.0	40.0	32.0	41.0
16	37.1727031248643	39.0	36.0	40.0	31.0	41.0
17	37.24792901904298	39.0	36.0	40.0	32.0	41.0
18	37.24189628284197	39.0	36.0	40.0	32.0	41.0
19	37.315183637110295	39.0	36.0	40.0	32.0	41.0
20	37.27668268365882	39.0	36.0	40.0	31.0	41.0
21	37.20901492396581	39.0	36.0	40.0	31.0	41.0
22	37.12882760828219	39.0	36.0	40.0	31.0	41.0
23	36.998067663417366	39.0	36.0	40.0	31.0	41.0
24	36.92489941304113	39.0	36.0	40.0	31.0	41.0
25	36.84431198272001	39.0	35.0	40.0	31.0	41.0
26	36.59512058648742	38.0	35.0	40.0	30.0	41.0
27	36.46847468856193	38.0	35.0	40.0	30.0	41.0
28	36.32629689870834	38.0	35.0	40.0	30.0	41.0
29	36.16776589633743	38.0	35.0	40.0	30.0	41.0
30	36.014132421196024	38.0	35.0	40.0	29.0	41.0
31	35.774634965180724	38.0	34.0	40.0	29.0	41.0
32	35.53670074774912	38.0	34.0	40.0	27.0	41.0
33	35.696510255961705	38.0	34.0	40.0	29.0	41.0
34	35.69961936381152	38.0	34.0	40.0	29.0	41.0
35	35.62264413121775	38.0	34.0	40.0	28.0	41.0
36	35.54300581003674	38.0	34.0	40.0	27.0	41.0
37	35.39275687049561	38.0	34.0	40.0	27.0	41.0
38	35.28094747146159	38.0	34.0	40.0	26.0	41.0
39	35.274191427249235	38.0	34.0	40.0	26.0	41.0
40	35.1409191346606	38.0	34.0	40.0	25.0	41.0
41	34.995287331936346	38.0	33.0	40.0	25.0	41.0
42	34.81500498127215	38.0	33.0	40.0	24.0	41.0
43	34.48847980010397	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	8.0
16	16.0
17	44.0
18	119.0
19	254.0
20	566.0
21	1097.0
22	2011.0
23	3540.0
24	5968.0
25	9498.0
26	14563.0
27	21219.0
28	29940.0
29	40332.0
30	52333.0
31	65957.0
32	81411.0
33	99730.0
34	121158.0
35	145556.0
36	178638.0
37	223649.0
38	265270.0
39	249157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.58579140193966	24.764304563540065	13.162096675140411	22.487807359379865
2	18.13759973400131	22.562061192105894	35.9320313789129	23.368307694979894
3	19.776022649590146	26.391685555799555	31.730641585372055	22.101650209238244
4	13.279029402532693	15.707197969278642	35.42943776759605	35.58433486059261
5	11.65809987109485	39.80607156903249	34.986023902662346	13.549804657210313
6	32.197379962507085	35.88104002511107	15.384376795087956	16.537203217293886
7	25.086815571345095	34.55898682289128	21.448067601384086	18.90613000437955
8	27.5690151224723	33.63661402522769	20.154673773198894	18.639697079101115
9	25.21969085096009	13.61611823046355	23.0949890759399	38.06920184263646
10	16.19161583039606	27.435891709748777	34.04770855277605	22.324783907079112
11	32.06853684590561	21.519343836807817	24.831362536118874	21.58075678116769
12	20.165219430311197	26.710908799916627	31.694290084973183	21.429581684798993
13	30.996911983464408	20.656026718973123	28.382333418939254	19.964727878623208
14	21.07487540616288	22.2770182836881	27.709768628282955	28.93833768186606
15	23.317874640672244	28.70062616389936	26.22276894217134	21.75873025325706
16	22.414546059087936	28.219247933361373	26.510107081842982	22.85609892570771
17	19.892955376982428	26.809107477615292	27.4910392931184	25.80689785228388
18	21.13033315591816	26.29280451205245	31.380525769243654	21.196336562785742
19	20.319123990873663	24.87292483179677	32.45531432881855	22.352636848511015
20	24.606491906518333	23.93603624728449	31.214028453423552	20.24344339277362
21	23.963516989053605	24.744826114520873	30.038994118004663	21.252662778420856
22	22.394633377128827	24.341609813168176	30.952744290147006	22.31101251955599
23	21.494592559232476	26.32555808237771	31.951976318176122	20.22787304021369
24	20.412856272618885	25.394500625915768	30.294757319616533	23.897885781848814
25	21.528462728546103	26.209803987250922	31.44051194822951	20.82122133597347
26	22.770244870158148	25.30709573843793	30.13675856276341	21.785900828640518
27	21.457620726062288	26.648503323122657	29.813627222187073	22.080248728627986
28	21.34602286050329	26.059001090545014	29.98651396555168	22.60846208340002
29	20.431342189203978	26.050626598132304	30.873651861804746	22.644379350858976
30	19.88240971987013	26.28964081491876	32.58763130893937	21.24031815627175
31	21.180766210225812	25.70832697492243	31.52711040310464	21.583796411747116
32	22.068648505804454	25.878174087707613	29.66232805926411	22.390849347223824
33	22.940526215883246	25.781774375045753	29.417420681150197	21.860278727920807
34	21.416740796432837	28.421042183869115	29.336777420879656	20.82543959881839
35	20.269497369168718	29.467171369409407	29.462518873624568	20.80081238779731
36	21.275739157513655	25.896846104124094	31.440387881675246	21.387026856687
37	21.229586399328053	26.26538580356046	30.542146028815697	21.962881768295784
38	21.381133695359537	25.80323788893314	31.26626047276801	21.54936794293931
39	22.270504789589328	25.33234328223032	31.22252701239053	21.174624915789828
40	20.733134082447187	26.18772014059222	30.338552813271153	22.74059296368944
41	21.683297788265538	25.642199501500585	30.340661944693608	22.333840765540266
42	19.31424693462561	27.255623006405553	30.74859277510828	22.681537283860553
43	19.96894614146813	27.03943703560338	30.350215069371817	22.64140175355668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	169.5
2	209.0
3	557.0
4	905.0
5	905.0
6	1584.5
7	2264.0
8	2592.0
9	2920.0
10	4441.5
11	5963.0
12	5963.0
13	10835.0
14	15707.0
15	25192.0
16	34677.0
17	34121.5
18	33566.0
19	33566.0
20	36465.0
21	39364.0
22	32834.5
23	26305.0
24	28989.0
25	31673.0
26	31673.0
27	35072.5
28	38472.0
29	40687.5
30	42903.0
31	46321.0
32	49739.0
33	49739.0
34	54078.5
35	58418.0
36	63099.0
37	67780.0
38	71636.5
39	75493.0
40	75493.0
41	79394.0
42	83295.0
43	94212.5
44	105130.0
45	129737.0
46	154344.0
47	154344.0
48	157739.5
49	161135.0
50	148674.0
51	136213.0
52	128494.5
53	120776.0
54	120776.0
55	105481.0
56	90186.0
57	75852.5
58	61519.0
59	54572.0
60	47625.0
61	47625.0
62	42249.0
63	36873.0
64	32345.5
65	27818.0
66	24061.5
67	20305.0
68	20305.0
69	17487.5
70	14670.0
71	12642.5
72	10615.0
73	8558.0
74	6501.0
75	6501.0
76	5190.0
77	3879.0
78	2999.0
79	2119.0
80	1712.5
81	1306.0
82	1306.0
83	929.0
84	552.0
85	438.5
86	325.0
87	272.5
88	220.0
89	220.0
90	166.0
91	112.0
92	67.5
93	23.0
94	14.0
95	5.0
96	5.0
97	4.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1612038.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.26622587952746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.84612629642378	37.090724897898845
2	12.821343891101344	12.37675760653994
3	4.347100253574929	6.294543682799958
4	1.909984030242594	3.6875088251991697
5	1.0303062287588447	2.486449658117924
6	0.6001836445648454	1.738115961465891
7	0.4058968267786962	1.3713775547558789
8	0.29213270135340763	1.1280114360256086
9	0.22756430201071703	0.9885303002669645
>10	1.3334739514539533	12.120564334164161
>50	0.11427595197118054	3.774791881930261
>100	0.059503125411003284	5.1483170681185895
>500	0.0062497013444042	2.194562932636722
>1k	0.0046872760083031505	4.317798328016702
>5k	6.510105567087709E-4	1.7678545819994735
>10k+	5.208084453670167E-4	3.514090950063865
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16924	1.0498511821681622	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14997	0.9303130571363702	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13929	0.8640615171602655	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10068	0.624551034156763	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5863	0.36370110382013326	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5828	0.3615299391205418	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5596	0.34713821882610707	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5557	0.3447189210179909	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5287	0.32796993619257114	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3955	0.2453416110538337	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3872	0.24019284905194543	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3859	0.23938641644924005	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3726	0.23113599059079254	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3333	0.2067569126782371	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3170	0.19664548850585406	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3072	0.19056622734699802	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2415	0.14981036427180996	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2274	0.141063672196313	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2181	0.13529457742311285	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2092	0.12977361575843746	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2076	0.1287810833243385	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1915	0.11879372570621784	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1902	0.11798729310351244	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1834	0.11376903025859192	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1646	0.10210677415792928	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1638	0.10161050794087981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.203327713118425E-5	0.0
5	0.0	0.0	0.0	6.203327713118425E-5	0.0
6	0.0	0.0	0.0	6.203327713118425E-5	1.240665542623685E-4
7	0.0	0.0	0.0	6.203327713118425E-5	1.240665542623685E-4
8	0.0	0.0	0.0	6.203327713118425E-5	1.240665542623685E-4
9	0.0	0.0	0.0	6.203327713118425E-5	1.240665542623685E-4
10	0.0	0.0	0.0	1.240665542623685E-4	1.240665542623685E-4
11	0.0	0.0	0.0	3.721996627871055E-4	1.240665542623685E-4
12	0.0	0.0	0.0	6.203327713118425E-4	1.8609983139355275E-4
13	0.0	0.0	0.0	6.823660484430268E-4	2.48133108524737E-4
14	0.0	0.0	0.0	8.684658798365796E-4	2.48133108524737E-4
15	0.0	0.0	0.0	0.001488798651148422	2.48133108524737E-4
16	0.0	0.0	0.0	0.0023572645309850016	2.48133108524737E-4
17	0.0	0.0	0.0	0.0035979300736086865	2.48133108524737E-4
18	0.0	0.0	0.0	0.004156229567789345	2.48133108524737E-4
19	0.0	0.0	0.0	0.004590462507707635	3.1016638565592127E-4
20	0.0	0.0	0.0	0.005707061496068951	3.1016638565592127E-4
21	0.0	0.0	0.0	0.00818839258131632	3.721996627871055E-4
22	0.0	0.0	0.0	0.013337154583204613	3.721996627871055E-4
23	0.0	0.0	0.0	0.023820778418374752	3.721996627871055E-4
24	0.0	0.0	0.0	0.04050772996666332	3.721996627871055E-4
25	0.0	0.0	0.0	0.046711057679781745	3.721996627871055E-4
26	0.0	0.0	0.0	0.0563262156351153	3.721996627871055E-4
27	0.0	0.0	0.0	0.11922795864613614	4.342329399182898E-4
28	0.0	0.0	0.0	0.1788419379692042	4.342329399182898E-4
29	0.0	0.0	0.0	0.25477066917777375	4.342329399182898E-4
30	0.0	0.0	0.0	0.3865913830815403	4.342329399182898E-4
31	0.0	0.0	0.0	0.5731254474150113	4.342329399182898E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3920	0.0	28.457907	1
ATTGGAC	590	0.0	25.084745	3
GTATTGG	595	0.0	24.252102	1
TATTGGA	730	0.0	20.020546	2
TTGGACC	1025	0.0	19.492682	4
TATACCG	105	2.2611857E-8	19.380953	5
TGGACCC	1105	0.0	19.253393	5
TAGACCG	50	0.00703608	18.5	5
GGACCCT	1160	0.0	18.340517	6
CGACGAG	95	3.6082092E-6	17.526316	24
TGCGACG	85	2.7242162E-5	17.411764	22
TTACGCT	65	0.001580307	17.076923	4
GTATCAA	6790	0.0	16.919733	2
GACCCTC	1205	0.0	16.580914	7
ACGGACC	125	1.6588092E-7	16.279999	8
GTATACG	115	1.2428773E-6	16.086956	1
CGTCTGT	300	0.0	16.033333	34
GCGCGAT	130	2.5906593E-7	15.653846	13
CGACGAA	160	1.0972144E-8	15.03125	10
ACCCTCG	1405	0.0	14.879004	8
>>END_MODULE