FastQCFastQC Report
Wed 25 May 2016
SRR2088246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462931
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT246191.6828544886942722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT216811.4820247844908612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT191121.3064184161795738No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG135310.9249240053016855No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT88700.6063170443445385No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA79030.5402168660039331No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT78460.5363205783458004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78460.5363205783458004No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA72360.49462346481139574No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA60390.41280142399060515No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT58220.39796818852016946No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT56550.38655274924107835No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG55190.37725634360062094No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA48190.32940719692179604No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48190.32940719692179604No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT40140.27438067824114737No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39840.2723300005263406No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA34000.23241014101143526No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC31120.21272363494929014No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30480.20834885582436902No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29940.20465763593771683No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29250.19994107719366125No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG27310.18668002797124403No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT25580.1748544531491916No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC24710.16890748777625192No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG22720.15530465893470027No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA22670.1549628793155658No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC22550.1541426082296431No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22250.1520919305148363No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC21670.14812728693287652No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC20440.13971950830216873No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC20080.13725869504440058No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA19100.1305598145093651No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC18640.12741544201332805No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18250.12474956098407922No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA18240.12468120506025233No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC18130.1239292898981565No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG17630.12051149370681188No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT17370.11873423968731267No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG17260.11798232452521684No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA17080.11675191789633278No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA16900.1155215112674487No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG16840.11511137572448735No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA16560.11319740985733436No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA16420.11224042692375784No Hit
CTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAAA15410.10533647861724169No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA15300.10458456345514586No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15200.10390100421687695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC14960.10226046204503152No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT14950.1021921061212046No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC14650.10014142840639785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57000.031.2228071
GTATTGG3600.023.638891
ATTGGAC4000.022.6624983
AGTCCGC450.003826300120.55555534
CGACGCT450.003826300120.55555529
GTGCGAA450.003826300120.55555513
CATTGCG656.903778E-519.92307729
GTATCAA98250.018.8106862
ACGCATA609.23846E-418.57
ACCCGTT701.21948615E-418.530
CCGTTGA609.23846E-418.532
GTGCACC500.007035778418.49999811
GCGTCGG500.007035778418.49999811
TTGGACC9300.018.3010754
TATTGGA5150.017.9611642
TGGACCC9650.017.8290165
AGTATCA3550.017.718311
CACGGCT953.607749E-617.5263164
AACCCGT852.7239386E-517.41176429
TATACCG852.7239386E-517.4117645
Produced by FastQC (version 0.11.5)