FastQCFastQC Report
Wed 25 May 2016
SRR2088245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414115
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT154051.089373919377137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT138900.9822397754072334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121240.8573560141855507No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG72600.5133953037765671No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52280.3697011912043929No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT46780.330807607584956No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA42720.3020970713131535No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41710.29495479504849326No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT41560.2938940609497813No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA32880.23251291443765182No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT31780.22473419771376446No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT30960.2189355179741393No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG30410.21504615961219561No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26220.18541632045484277No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA25890.18308270543767657No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA22940.16222160149634224No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT22310.1577665182817522No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19070.13485466174957483No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC18790.1328746247653126No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17660.12488376122168282No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16290.11519572312011399No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15270.10798273124887298No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.10557840062512597No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC14470.10232548272240943No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT14460.10225476711582862No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14250.10076973937763194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG250.00549676729.5999982
GGTATCA38900.028.8676091
CGCCGGA400.001931405923.12514
GTATTGG3800.021.4210531
ATTGGAC3850.020.6623363
GGCGTCG450.003826236420.5555578
ATAACGC450.003826236420.5555573
TTAGACC1205.176844E-918.54
CGTTTAG902.1529286E-618.526
CTACGCT500.007035664718.49999825
ACCGTGT1002.876095E-718.4999988
CCGGTAC500.007035664718.4999983
GTATCAA65700.017.5707762
TTGGACC6750.017.5407414
GTATTAG1201.04168066E-716.9583321
GGACCCT7100.016.6760586
CGTCTGT4000.016.6534
CGTGTCT1606.2937033E-1016.187535
TGGACCC7150.016.0419585
AGTATCA2200.015.9772731
Produced by FastQC (version 0.11.5)