##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088245_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1414115
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.967288374707856	31.0	30.0	33.0	27.0	34.0
2	30.84494047513816	31.0	31.0	34.0	27.0	34.0
3	30.888151246539355	31.0	31.0	34.0	27.0	34.0
4	35.065377992596076	35.0	35.0	37.0	32.0	37.0
5	34.96269468890437	35.0	35.0	37.0	32.0	37.0
6	35.07171906103817	36.0	35.0	37.0	32.0	37.0
7	34.69470163317693	35.0	35.0	37.0	31.0	37.0
8	34.90835752396375	36.0	35.0	37.0	32.0	37.0
9	36.36331345046195	38.0	35.0	39.0	32.0	39.0
10	36.28461970914671	38.0	35.0	39.0	32.0	39.0
11	36.37198601245302	38.0	35.0	39.0	32.0	39.0
12	36.11750458767498	38.0	35.0	39.0	31.0	39.0
13	36.28708061225572	38.0	35.0	39.0	32.0	39.0
14	37.22089009734003	39.0	36.0	40.0	32.0	41.0
15	37.26742520940659	39.0	36.0	40.0	32.0	41.0
16	37.193973616007185	39.0	36.0	40.0	32.0	41.0
17	37.266227994187176	39.0	36.0	40.0	32.0	41.0
18	37.268495136534156	39.0	36.0	40.0	32.0	41.0
19	37.344105677402474	39.0	36.0	40.0	32.0	41.0
20	37.30344066783819	39.0	36.0	40.0	31.0	41.0
21	37.24596231565325	39.0	36.0	40.0	31.0	41.0
22	37.166627890942394	39.0	36.0	40.0	31.0	41.0
23	37.050226466730074	39.0	36.0	40.0	31.0	41.0
24	36.97910778119177	39.0	36.0	40.0	31.0	41.0
25	36.8968429017442	39.0	35.0	40.0	31.0	41.0
26	36.66445373961807	38.0	35.0	40.0	30.0	41.0
27	36.52683692627544	38.0	35.0	40.0	30.0	41.0
28	36.373529026988614	38.0	35.0	40.0	30.0	41.0
29	36.210394487011314	38.0	35.0	40.0	30.0	41.0
30	36.06105444040973	38.0	35.0	40.0	30.0	41.0
31	35.823949254480716	38.0	34.0	40.0	29.0	41.0
32	35.59953327699657	38.0	34.0	40.0	28.0	41.0
33	35.730962474763366	38.0	34.0	40.0	29.0	41.0
34	35.74100055511751	38.0	34.0	40.0	29.0	41.0
35	35.66536455663083	38.0	34.0	40.0	28.0	41.0
36	35.59252536038441	38.0	34.0	40.0	27.0	41.0
37	35.43742411331469	38.0	34.0	40.0	27.0	41.0
38	35.32523026769393	38.0	34.0	40.0	27.0	41.0
39	35.302582180374294	38.0	34.0	40.0	26.0	41.0
40	35.168470739649884	38.0	34.0	40.0	25.0	41.0
41	35.02006908914763	38.0	34.0	40.0	25.0	41.0
42	34.847513816061635	38.0	33.0	40.0	24.0	41.0
43	34.51115503336009	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	15.0
17	30.0
18	92.0
19	211.0
20	460.0
21	900.0
22	1630.0
23	3020.0
24	4991.0
25	8323.0
26	12476.0
27	18266.0
28	25715.0
29	35134.0
30	45847.0
31	57393.0
32	71047.0
33	86968.0
34	105774.0
35	127340.0
36	155623.0
37	195577.0
38	231102.0
39	226179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.01606305003483	25.07518836869703	13.544584422059025	22.364164159209114
2	18.026044557903706	22.841635934842643	35.79376500496777	23.338554502285884
3	19.734109319256213	25.9895411617867	31.98728533393677	22.28906418502031
4	13.436884553236478	16.087871212737294	35.185115779126875	35.29012845489935
5	11.65683130438472	39.97114803251504	34.8348613797322	13.537159283368041
6	32.03890772674075	35.855287582693066	15.427387447272675	16.67841724329351
7	24.830088076287996	34.78387542738744	21.608780049713072	18.777256446611485
8	27.387376557069267	33.81280871781998	20.223814894828216	18.575999830282544
9	25.173341630631175	13.862946082885763	23.506079774275783	37.45763251220728
10	16.289198544672818	28.06023555368552	33.972767419905736	21.677798481735927
11	32.008005006664945	21.992907224659948	24.623386358252333	21.375701410422774
12	20.06159329333187	27.26659430102927	31.627555043260276	21.04425736237859
13	30.644466680574066	21.204074633251185	28.33079346446364	19.820665221711106
14	20.996029318690486	22.742987663662433	28.11808091986861	28.14290209777847
15	23.2350268542516	29.223224419513265	26.17912970302981	21.362619023205326
16	22.245008362120476	28.32343904137924	26.82808682462176	22.603465771878525
17	19.83007039738635	27.448262694335323	27.76952369503187	24.952143213246448
18	20.78084172786513	26.70256662294085	31.646365394610765	20.870226254583255
19	20.258677688872545	25.576491303748284	32.70264441010809	21.462186597271085
20	24.10277806260453	24.5296174639262	31.61397764679676	19.753626826672512
21	23.443779324878104	25.2737577919759	30.274411911336774	21.008050971809222
22	21.928768169491168	25.212871654709833	31.074841862224783	21.783518313574216
23	21.218288470173924	26.91994639757021	31.772451321144317	20.08931381111154
24	20.034014206765363	26.18443337352337	30.545323400147794	23.236229019563474
25	20.991927813508802	26.794779773922205	31.699897108792424	20.513395303776566
26	22.270607411702727	25.87476973230607	30.39674991072155	21.457872945269656
27	21.05033890454454	27.169713920013578	30.082489755076498	21.69745742036539
28	20.8048850341026	26.79852770107099	30.164590574316797	22.23199669050961
29	20.06138114651213	26.714871138485908	31.03799903119619	22.185748683805773
30	19.547349402276335	26.88416430064033	32.72760701923111	20.840879277852224
31	20.758424880579017	26.253239658726486	31.79005950718294	21.198275953511562
32	21.718459955519883	26.66890599420839	30.09910792262298	21.513526127648742
33	22.634368491954334	26.51382666897671	29.556082779689063	21.295722059379894
34	20.997302199608942	28.801335110652243	29.678279347860677	20.523083341878134
35	19.827595351156024	29.550213384342854	29.863554237102356	20.758637027398763
36	20.66317095851469	26.39700448690524	31.776977119965487	21.16284743461458
37	20.661685930776493	26.579026458244204	31.103481682890006	21.6558059280893
38	20.852547352938057	26.32232880635592	31.628545061752405	21.19657877895362
39	21.7857812129848	25.678251061618045	31.565325309469173	20.970642415927983
40	20.24029163116154	26.290011774148496	31.021593010469445	22.44810358422052
41	20.980542600849294	25.80080120782256	30.8056275479717	22.413028643356444
42	18.851154255488414	27.20132379615519	31.35409779261234	22.593424155744053
43	19.35005285991592	26.99525851857876	31.182046721801264	22.472641899704055
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	208.0
2	269.0
3	616.0
4	963.0
5	963.0
6	1638.5
7	2314.0
8	2576.5
9	2839.0
10	4420.5
11	6002.0
12	6002.0
13	10577.0
14	15152.0
15	23960.5
16	32769.0
17	31865.5
18	30962.0
19	30962.0
20	33674.0
21	36386.0
22	30596.5
23	24807.0
24	27442.0
25	30077.0
26	30077.0
27	32894.5
28	35712.0
29	37554.0
30	39396.0
31	42251.0
32	45106.0
33	45106.0
34	49050.0
35	52994.0
36	57556.5
37	62119.0
38	64914.5
39	67710.0
40	67710.0
41	71612.0
42	75514.0
43	84448.5
44	93383.0
45	114617.0
46	135851.0
47	135851.0
48	137900.5
49	139950.0
50	127406.5
51	114863.0
52	107178.0
53	99493.0
54	99493.0
55	88061.0
56	76629.0
57	64667.5
58	52706.0
59	47219.0
60	41732.0
61	41732.0
62	36477.5
63	31223.0
64	27213.5
65	23204.0
66	19495.0
67	15786.0
68	15786.0
69	13412.0
70	11038.0
71	9198.5
72	7359.0
73	5928.5
74	4498.0
75	4498.0
76	3415.0
77	2332.0
78	1859.0
79	1386.0
80	1057.0
81	728.0
82	728.0
83	530.5
84	333.0
85	273.5
86	214.0
87	155.5
88	97.0
89	97.0
90	75.0
91	53.0
92	32.5
93	12.0
94	9.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1414115.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.72166796375049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45155411066958	38.01298788803416
2	12.964892186236233	12.89272128939722
3	4.596177029636197	6.855887645105637
4	2.0494316471957266	4.076046395050725
5	1.0464615529312926	2.601590693584022
6	0.6437684942667968	1.920554599047437
7	0.41752499377988905	1.453202737510344
8	0.2696941317123575	1.0727713655019064
9	0.21946783650124954	0.9821076205714028
>10	1.1862329226751562	10.884656630590529
>50	0.08896532190739975	3.030036518921876
>100	0.05375621779679005	5.109923256875048
>500	0.006755441315446445	2.4991065330046713
>1k	0.004599449406261409	4.755870698386053
>5k	2.874655878913381E-4	0.892471685897862
>10k+	4.311983818370071E-4	2.960064442521104
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15405	1.089373919377137	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13890	0.9822397754072334	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12124	0.8573560141855507	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7260	0.5133953037765671	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5228	0.3697011912043929	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4678	0.330807607584956	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4272	0.3020970713131535	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4171	0.29495479504849326	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4156	0.2938940609497813	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3288	0.23251291443765182	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3178	0.22473419771376446	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3096	0.2189355179741393	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3041	0.21504615961219561	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2622	0.18541632045484277	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2589	0.18308270543767657	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2294	0.16222160149634224	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2231	0.1577665182817522	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1907	0.13485466174957483	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1879	0.1328746247653126	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1766	0.12488376122168282	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1629	0.11519572312011399	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1527	0.10798273124887298	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1493	0.10557840062512597	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1447	0.10232548272240943	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1446	0.10225476711582862	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1425	0.10076973937763194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.071560658079434E-5	0.0
8	0.0	0.0	0.0	7.071560658079434E-5	0.0
9	0.0	0.0	0.0	7.071560658079434E-5	0.0
10	0.0	0.0	0.0	7.071560658079434E-5	0.0
11	0.0	0.0	0.0	1.414312131615887E-4	0.0
12	0.0	0.0	0.0	2.828624263231774E-4	0.0
13	0.0	0.0	0.0	3.5357803290397177E-4	0.0
14	0.0	0.0	0.0	4.950092460655604E-4	0.0
15	0.0	0.0	0.0	8.485872789695322E-4	0.0
16	0.0	0.0	0.0	0.001414312131615887	0.0
17	0.0	0.0	0.0	0.002970055476393363	0.0
18	0.0	0.0	0.0	0.0031114866895549514	0.0
19	0.0	0.0	0.0	0.0035357803290397174	0.0
20	0.0	0.0	0.0	0.004313652001428455	0.0
21	0.0	0.0	0.0	0.006859413838337052	0.0
22	0.0	0.0	0.0	0.011668075085831068	0.0
23	0.0	0.0	0.0	0.0200125166623648	0.0
24	0.0	0.0	0.0	0.03245846342058461	0.0
25	0.0	0.0	0.0	0.0400957489313104	0.0
26	0.0	0.0	0.0	0.05105666795133352	0.0
27	0.0	0.0	0.0	0.13584468024170596	0.0
28	0.0	0.0	0.0	0.2187233711543969	0.0
29	0.0	0.0	0.0	0.3066935857409051	0.0
30	0.0	0.0	0.0	0.46198505779232946	0.0
31	0.0	0.0	0.0	0.6818398786520191	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAACG	25	0.005496767	29.599998	2
GGTATCA	3890	0.0	28.867609	1
CGCCGGA	40	0.0019314059	23.125	14
GTATTGG	380	0.0	21.421053	1
ATTGGAC	385	0.0	20.662336	3
GGCGTCG	45	0.0038262364	20.555557	8
ATAACGC	45	0.0038262364	20.555557	3
TTAGACC	120	5.176844E-9	18.5	4
CGTTTAG	90	2.1529286E-6	18.5	26
CTACGCT	50	0.0070356647	18.499998	25
ACCGTGT	100	2.876095E-7	18.499998	8
CCGGTAC	50	0.0070356647	18.499998	3
GTATCAA	6570	0.0	17.570776	2
TTGGACC	675	0.0	17.540741	4
GTATTAG	120	1.04168066E-7	16.958332	1
GGACCCT	710	0.0	16.676058	6
CGTCTGT	400	0.0	16.65	34
CGTGTCT	160	6.2937033E-10	16.1875	35
TGGACCC	715	0.0	16.041958	5
AGTATCA	220	0.0	15.977273	1
>>END_MODULE