FastQCFastQC Report
Wed 25 May 2016
SRR2088244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537228
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79071.4718145740728332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73091.3605024309976397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63341.1790152412011288No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG38850.7231566485737899No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25810.4804291660151742No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT24610.4580922811171421No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT23490.4372445218789788No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22680.42216712457280703No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22360.41621062193333186No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA19350.36018226898076794No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT16920.31495007706225286No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT16690.3106688407901301No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG16010.2980112726812452No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13710.255198909960017No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13630.2537097843001482No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12840.2390046684089437No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT12200.2270916631299932No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA10360.19284177295301064No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.18949124021830582No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC9920.1846515818237322No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA8690.16175627480324925No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG8350.15542749074880685No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8300.15449678721138882No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC8070.150215550939266No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT7300.1358827164630287No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.13346288726574193No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC7080.1317876208983895No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC6590.12266672623169306No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA6030.1122428466126114No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA5980.11131214307519341No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC5970.11112600236770981No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.10833389175545578No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC5700.10610020326565256No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG5630.10479721831326737No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG5630.10479721831326737No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA5490.10219124840849694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG1250.029.6000021
GGTATCA18950.029.5804751
GCAATAC405.934332E-527.753
ATTGGAC1700.023.9411753
TAATGGG400.00192985623.1255
CGTCTGT2000.023.12499834
CTAGGCA603.7215323E-521.5833324
TTGGACC3950.020.6075954
GACAATC450.003823195820.55555522
GGACCCT4050.020.0987646
GACCCTC4400.018.9204547
TGGACCC4350.018.7126455
TCTAGGA609.228088E-418.52
TAGGAGT609.228088E-418.54
GTATTGT500.00703012518.4999981
GATTGGC500.00703012518.4999989
ATTATAC500.00703012518.4999983
TAGGAGA500.00703012518.4999984
GTGCCCG500.00703012518.4999989
GTATCAA31250.018.41122
Produced by FastQC (version 0.11.5)