##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088244_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	537228
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.941736097150557	31.0	30.0	33.0	27.0	34.0
2	30.81010669585353	31.0	31.0	34.0	27.0	34.0
3	30.76856753557149	31.0	31.0	34.0	27.0	34.0
4	34.98558898642662	35.0	35.0	37.0	32.0	37.0
5	34.91816137654776	35.0	35.0	37.0	32.0	37.0
6	35.05451130618657	36.0	35.0	37.0	32.0	37.0
7	34.62788797307661	35.0	35.0	37.0	31.0	37.0
8	34.87821185790763	35.0	35.0	37.0	32.0	37.0
9	36.27036937761993	38.0	35.0	39.0	32.0	39.0
10	36.23330690135287	38.0	35.0	39.0	32.0	39.0
11	36.34098743922506	38.0	35.0	39.0	32.0	39.0
12	36.05329208455255	38.0	35.0	39.0	31.0	39.0
13	36.21740676211962	38.0	35.0	39.0	32.0	39.0
14	37.082341203362446	39.0	36.0	40.0	31.0	41.0
15	37.13676874623065	39.0	36.0	40.0	31.0	41.0
16	37.06128682793898	39.0	36.0	40.0	31.0	41.0
17	37.203405258102705	39.0	36.0	40.0	32.0	41.0
18	37.21628619506057	39.0	36.0	40.0	32.0	41.0
19	37.30630756401379	39.0	36.0	40.0	31.0	41.0
20	37.29876700395363	39.0	36.0	40.0	31.0	41.0
21	37.222938863946034	39.0	36.0	40.0	31.0	41.0
22	37.152728450490294	39.0	36.0	40.0	31.0	41.0
23	37.01363480682318	39.0	36.0	40.0	31.0	41.0
24	36.93719612529503	39.0	35.0	40.0	31.0	41.0
25	36.85708302620117	39.0	35.0	40.0	31.0	41.0
26	36.59588293983188	38.0	35.0	40.0	30.0	41.0
27	36.46113195887035	38.0	35.0	40.0	30.0	41.0
28	36.2996269740222	38.0	35.0	40.0	30.0	41.0
29	36.11955259219549	38.0	35.0	40.0	30.0	41.0
30	35.93066817068358	38.0	34.0	40.0	30.0	41.0
31	35.63549926660561	38.0	34.0	40.0	29.0	41.0
32	35.39434467302523	38.0	34.0	40.0	27.0	41.0
33	35.47864966085163	38.0	34.0	40.0	28.0	41.0
34	35.448247299098334	38.0	34.0	40.0	27.0	41.0
35	35.342584526495266	38.0	34.0	40.0	27.0	41.0
36	35.231856865241575	38.0	34.0	40.0	26.0	41.0
37	35.053937992807526	38.0	33.0	40.0	25.0	41.0
38	34.91386152620489	38.0	33.0	40.0	25.0	41.0
39	34.854009843120615	38.0	33.0	40.0	25.0	41.0
40	34.660596618195626	38.0	33.0	40.0	24.0	41.0
41	34.46162150893103	38.0	33.0	40.0	23.0	41.0
42	34.222728524946575	38.0	33.0	40.0	22.0	41.0
43	33.85524209460415	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	3.0
17	12.0
18	39.0
19	98.0
20	188.0
21	420.0
22	718.0
23	1232.0
24	2236.0
25	3311.0
26	5141.0
27	7596.0
28	10594.0
29	14440.0
30	18384.0
31	23041.0
32	27901.0
33	34109.0
34	41258.0
35	49356.0
36	60089.0
37	75585.0
38	84839.0
39	76636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.01043877087568	25.720550678669017	14.173498030631315	21.095512519823988
2	17.85368595828959	23.17954388081038	36.33801663353362	22.628753527366406
3	19.888576172500315	26.541058917256734	32.80971952318196	20.760645387060986
4	13.086622439634569	15.766862486690938	34.90566388944731	36.24085118422718
5	10.75539621910995	41.095400835399495	34.628500376004226	13.520702569486327
6	31.33269300929959	35.6196996433544	15.211790896974842	17.835816450371166
7	23.630562815043145	36.36240106621397	21.07969055968788	18.927345559055002
8	28.111528066295875	33.46288726574192	19.958751219221636	18.466833448740573
9	24.850901293305636	13.801588897079082	24.666994274311836	36.68051553530345
10	16.504724251155935	28.32223934716731	33.28512289009508	21.887913511581676
11	30.613259174875473	21.72280670404372	25.547625961416752	22.11630815966405
12	20.01738554207897	28.043214426649392	31.636660784620315	20.302739246651328
13	31.38965206578957	21.158614219660926	29.435546918626727	18.016186795922774
14	20.631463736067367	23.26777457615761	28.965355491523155	27.13540619625187
15	22.974044539748487	29.045209855033615	27.513085691736094	20.4676599134818
16	21.602187525594346	28.48380948126308	27.60745903043028	22.306543962712293
17	18.59098930063213	27.406054784933026	28.181330831602224	25.821625082832618
18	19.964521581153626	26.568793882671788	33.3337056147483	20.132978921426282
19	18.973508454510934	24.88887399763229	34.86359608955601	21.27402145830076
20	24.274981944351374	23.55889864266196	33.3746565703947	18.791462842591972
21	23.50138116404953	24.68728361142755	31.787248616974544	20.024086607548377
22	21.431496496831883	24.29340987439225	32.9137721786653	21.36132145011057
23	20.367143931440655	26.866619014645547	34.00046162895456	18.765775424959237
24	18.76242489222453	25.73637263880513	32.22560998309842	23.275592485871922
25	20.124230308174557	26.71565890087635	33.764435211865354	19.39567557908374
26	21.866135048806093	25.438919788246334	31.980276530635038	20.714668632312538
27	20.205387656637406	27.253605545503955	31.52125354598048	21.01975325187816
28	19.867914553969637	26.84632967752984	31.54191516451116	21.743840603989366
29	18.842465396442478	26.589827782617437	32.736566225140905	21.831140595799177
30	18.037965258698353	26.91352647293142	35.16179350294475	19.88671476542548
31	19.74096659146582	26.056348514969436	33.750288518096596	20.452396375468144
32	21.21147818058627	26.50476147929743	31.30067680761241	20.98308353250389
33	22.367598114766913	26.311919706344423	30.702792855175083	20.61768932371358
34	20.075647583521334	29.747891025784213	30.74876960992353	19.42769178077092
35	18.483399971706614	30.737042745352067	30.932862769624812	19.846694513316507
36	19.70690284199632	26.315084098371642	33.68625611472224	20.291756944909796
37	19.723283224254878	26.87667061284967	32.50537946644628	20.89466669644918
38	20.155315806324317	26.412994110508016	33.050771739373225	20.38091834379444
39	21.32316260507643	25.734697372437775	33.03122696508745	19.91091305739835
40	19.31247068283857	26.639713492223045	32.0000074456283	22.047808379310087
41	20.302366965236363	26.123545310371014	31.90042216712457	21.673665557268052
42	17.59681178196222	27.933949831356518	32.27046989360197	22.19876849307929
43	18.2769699271073	27.710394841668712	32.04095840127469	21.971676829949295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	56.0
2	72.0
3	228.5
4	385.0
5	385.0
6	703.0
7	1021.0
8	1122.0
9	1223.0
10	1928.5
11	2634.0
12	2634.0
13	4826.0
14	7018.0
15	11667.0
16	16316.0
17	15739.5
18	15163.0
19	15163.0
20	16259.5
21	17356.0
22	13703.5
23	10051.0
24	10875.5
25	11700.0
26	11700.0
27	12506.5
28	13313.0
29	13761.5
30	14210.0
31	15009.5
32	15809.0
33	15809.0
34	17016.5
35	18224.0
36	20016.0
37	21808.0
38	22741.0
39	23674.0
40	23674.0
41	25202.0
42	26730.0
43	31259.0
44	35788.0
45	46934.0
46	58080.0
47	58080.0
48	59231.0
49	60382.0
50	53699.0
51	47016.0
52	43626.5
53	40237.0
54	40237.0
55	33783.0
56	27329.0
57	21544.0
58	15759.0
59	13537.0
60	11315.0
61	11315.0
62	9764.5
63	8214.0
64	7040.5
65	5867.0
66	4871.5
67	3876.0
68	3876.0
69	3205.0
70	2534.0
71	2114.0
72	1694.0
73	1355.0
74	1016.0
75	1016.0
76	800.0
77	584.0
78	454.0
79	324.0
80	274.5
81	225.0
82	225.0
83	168.0
84	111.0
85	83.5
86	56.0
87	47.0
88	38.0
89	38.0
90	33.0
91	28.0
92	17.0
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	537228.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.448440923646444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.15523112192056	42.26758554944649
2	10.283581014252526	10.581484205906065
3	2.953033836028617	4.55786960575342
4	1.3423532026447764	2.7624791777974953
5	0.7269407384233476	1.8699983817882762
6	0.4746331091201992	1.4651480084986321
7	0.31860194116096424	1.1474101203585263
8	0.24950852008486565	1.0269459484426129
9	0.1851251722863385	0.8571961340868202
>10	1.1432582612922038	10.948543187469133
>50	0.08772036499641334	3.1028813842881484
>100	0.06533148516728739	7.070989303407196
>500	0.0077076471264777265	2.6130521278787797
>1k	0.005872493048744934	5.659095188639916
>5k	0.0011010924466396752	4.069321676238453
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7907	1.4718145740728332	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7309	1.3605024309976397	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6334	1.1790152412011288	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3885	0.7231566485737899	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2581	0.4804291660151742	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2461	0.4580922811171421	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2349	0.4372445218789788	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2268	0.42216712457280703	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2236	0.41621062193333186	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1935	0.36018226898076794	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1692	0.31495007706225286	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1669	0.3106688407901301	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1601	0.2980112726812452	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1371	0.255198909960017	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1363	0.2537097843001482	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1284	0.2390046684089437	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1220	0.2270916631299932	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1036	0.19284177295301064	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1018	0.18949124021830582	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	992	0.1846515818237322	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	869	0.16175627480324925	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	835	0.15542749074880685	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	830	0.15449678721138882	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	807	0.150215550939266	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	730	0.1358827164630287	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	717	0.13346288726574193	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	708	0.1317876208983895	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	659	0.12266672623169306	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	603	0.1122428466126114	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	598	0.11131214307519341	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	597	0.11112600236770981	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	582	0.10833389175545578	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	570	0.10610020326565256	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	563	0.10479721831326737	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	563	0.10479721831326737	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	549	0.10219124840849694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.86140707483601E-4	0.0
3	0.0	0.0	0.0	1.86140707483601E-4	0.0
4	0.0	0.0	0.0	1.86140707483601E-4	0.0
5	0.0	0.0	0.0	1.86140707483601E-4	0.0
6	0.0	0.0	0.0	1.86140707483601E-4	0.0
7	0.0	0.0	0.0	1.86140707483601E-4	0.0
8	1.86140707483601E-4	0.0	0.0	1.86140707483601E-4	0.0
9	1.86140707483601E-4	0.0	0.0	3.72281414967202E-4	0.0
10	1.86140707483601E-4	0.0	0.0	3.72281414967202E-4	0.0
11	1.86140707483601E-4	0.0	0.0	3.72281414967202E-4	0.0
12	1.86140707483601E-4	0.0	0.0	3.72281414967202E-4	0.0
13	1.86140707483601E-4	0.0	0.0	7.44562829934404E-4	0.0
14	1.86140707483601E-4	0.0	0.0	9.30703537418005E-4	0.0
15	1.86140707483601E-4	0.0	0.0	0.001675266367352409	0.0
16	1.86140707483601E-4	0.0	0.0	0.002605969904770414	0.0
17	1.86140707483601E-4	0.0	0.0	0.004653517687090025	0.0
18	1.86140707483601E-4	0.0	0.0	0.005398080517024429	0.0
19	1.86140707483601E-4	0.0	0.0	0.007073346884376838	0.0
20	1.86140707483601E-4	0.0	0.0	0.00949317608166365	0.0
21	1.86140707483601E-4	0.0	0.0	0.013215990231335671	0.0
22	1.86140707483601E-4	0.0	0.0	0.021778462775581316	0.0
23	1.86140707483601E-4	0.0	0.0	0.03555287512936779	0.0
24	1.86140707483601E-4	0.0	0.0	0.05770361931991631	0.0
25	1.86140707483601E-4	0.0	0.0	0.06552152903422755	0.0
26	1.86140707483601E-4	0.0	0.0	0.0763176900682764	0.0
27	1.86140707483601E-4	0.0	0.0	0.16026714914338047	0.0
28	1.86140707483601E-4	0.0	0.0	0.2423552011436485	0.0
29	1.86140707483601E-4	0.0	0.0	0.33784538408273584	0.0
30	1.86140707483601E-4	0.0	0.0	0.5453922729269509	0.0
31	1.86140707483601E-4	0.0	0.0	0.7801157050637718	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	125	0.0	29.600002	1
GGTATCA	1895	0.0	29.580475	1
GCAATAC	40	5.934332E-5	27.75	3
ATTGGAC	170	0.0	23.941175	3
TAATGGG	40	0.001929856	23.125	5
CGTCTGT	200	0.0	23.124998	34
CTAGGCA	60	3.7215323E-5	21.583332	4
TTGGACC	395	0.0	20.607595	4
GACAATC	45	0.0038231958	20.555555	22
GGACCCT	405	0.0	20.098764	6
GACCCTC	440	0.0	18.920454	7
TGGACCC	435	0.0	18.712645	5
TCTAGGA	60	9.228088E-4	18.5	2
TAGGAGT	60	9.228088E-4	18.5	4
GTATTGT	50	0.007030125	18.499998	1
GATTGGC	50	0.007030125	18.499998	9
ATTATAC	50	0.007030125	18.499998	3
TAGGAGA	50	0.007030125	18.499998	4
GTGCCCG	50	0.007030125	18.499998	9
GTATCAA	3125	0.0	18.4112	2
>>END_MODULE