FastQCFastQC Report
Wed 25 May 2016
SRR2088243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633890
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78161.2330215021533704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72861.14941078105034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62520.986290996860654No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG32840.5180709586836832No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.4041710706904984No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA21410.3377557620407326No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT21060.33223429932638154No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT20090.31693195980375144No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18840.2972124501096405No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA16120.254302797015255No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT15310.2415245547334711No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT14720.23221694615785074No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG13880.21896543564340815No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA12890.2033475839656723No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11650.18378583034911422No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11090.17495149000615248No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT10730.1692722712142485No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9450.1490794932874789No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8910.14056066509962295No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC8330.1314108126015555No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7370.11626622915647826No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.11389988799318494No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG7160.11295335152786762No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG7140.11263783937276184No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC6730.10616984019309343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.029.2222211
CGACGAA451.3222975E-424.66666610
ATTGGAC1750.023.2571413
GTATTGG1800.021.5833321
TTGGACC3150.020.5555554
TATACCG450.003823943220.5555555
GTGCGAA450.003823943220.55555511
ACGACGC450.003823943220.55555526
AGTGCGA555.139598E-420.18181810
GCTTAGA555.139598E-420.1818181
TGGACCC3500.018.55
CGACGCT500.00703148718.49999827
GGACCCT3250.018.2153856
CGTCTGT2150.018.06976934
TATTGGA2600.017.7884622
CTGTACT752.0654239E-417.2666664
GTATCAA29000.017.0965522
CCGAATT650.001578877117.07692314
GACCCTC3650.016.7260277
TTAGAAC904.440896E-516.4444453
Produced by FastQC (version 0.11.5)