##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	633890
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.835608701825237	31.0	30.0	33.0	27.0	34.0
2	30.768341510356688	31.0	31.0	34.0	27.0	34.0
3	30.66113836785562	31.0	31.0	34.0	27.0	34.0
4	34.93116471312057	35.0	35.0	37.0	32.0	37.0
5	34.88454148196059	35.0	35.0	37.0	32.0	37.0
6	35.0043256716465	36.0	35.0	37.0	32.0	37.0
7	34.55138273201975	35.0	35.0	37.0	30.0	37.0
8	34.83899256968875	35.0	35.0	37.0	32.0	37.0
9	36.25674012841345	38.0	35.0	39.0	32.0	39.0
10	36.20508921106185	38.0	35.0	39.0	32.0	39.0
11	36.302629793812805	38.0	35.0	39.0	32.0	39.0
12	36.028694252946096	38.0	35.0	39.0	30.0	39.0
13	36.20239946993958	38.0	35.0	39.0	32.0	39.0
14	37.110683241571884	39.0	36.0	40.0	31.0	41.0
15	37.16375238606067	39.0	36.0	40.0	32.0	41.0
16	37.100741453564495	39.0	36.0	40.0	31.0	41.0
17	37.186404581236495	39.0	36.0	40.0	31.0	41.0
18	37.19543296155484	39.0	36.0	40.0	31.0	41.0
19	37.283277855779396	39.0	36.0	40.0	31.0	41.0
20	37.24083989335689	39.0	36.0	40.0	31.0	41.0
21	37.18624051491584	39.0	36.0	40.0	31.0	41.0
22	37.10338702298506	39.0	36.0	40.0	31.0	41.0
23	36.96506807174747	39.0	36.0	40.0	31.0	41.0
24	36.899821735632365	39.0	35.0	40.0	31.0	41.0
25	36.814665004969314	39.0	35.0	40.0	30.0	41.0
26	36.56249033744025	38.0	35.0	40.0	30.0	41.0
27	36.42406095694837	38.0	35.0	40.0	30.0	41.0
28	36.266087176008455	38.0	35.0	40.0	30.0	41.0
29	36.114281657700865	38.0	35.0	40.0	30.0	41.0
30	35.932145955922955	38.0	34.0	40.0	29.0	41.0
31	35.67468330467431	38.0	34.0	40.0	29.0	41.0
32	35.39824259729606	38.0	34.0	40.0	27.0	41.0
33	35.53641799050308	38.0	34.0	40.0	28.0	41.0
34	35.535384688195116	38.0	34.0	40.0	28.0	41.0
35	35.44810140560665	38.0	34.0	40.0	27.0	41.0
36	35.36434554891227	38.0	34.0	40.0	27.0	41.0
37	35.19817633974349	38.0	34.0	40.0	26.0	41.0
38	35.07190048746628	38.0	34.0	40.0	25.0	41.0
39	35.033715628894605	38.0	33.0	40.0	25.0	41.0
40	34.882569530991184	38.0	33.0	40.0	24.0	41.0
41	34.721256053889476	38.0	33.0	40.0	24.0	41.0
42	34.5187272239663	38.0	33.0	40.0	23.0	41.0
43	34.180670147817445	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	2.0
16	10.0
17	19.0
18	54.0
19	95.0
20	238.0
21	449.0
22	859.0
23	1567.0
24	2592.0
25	4039.0
26	6166.0
27	8921.0
28	12491.0
29	16850.0
30	21555.0
31	27064.0
32	32872.0
33	39748.0
34	47949.0
35	57631.0
36	70199.0
37	87926.0
38	99613.0
39	94980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.79048415340201	25.52919276215116	13.740869867011627	21.9394532174352
2	17.745823407846785	23.238890028238337	36.20328448153465	22.81200208238022
3	19.49849342945937	26.921232390477844	32.30181892757418	21.2784552524886
4	13.043903516382969	16.3889633848144	35.74594961270883	34.8211834860938
5	11.239331745255486	40.61903484831753	34.86756377289435	13.274069633532632
6	31.297543737872502	36.482197226648154	15.491015791383363	16.72924324409598
7	23.96614554575715	35.4192367760968	22.045149789395637	18.569467888750413
8	27.325087949013238	34.059063875435804	20.357633027812398	18.258215147738564
9	24.87024562621275	14.138572938522456	23.97608417864298	37.01509725662181
10	16.384230702487812	28.107242581520453	34.12611020839578	21.382416507595956
11	31.12133019924593	22.410986133240783	25.1898594393349	21.277824228178392
12	19.88089416144757	27.961318209784032	31.771127482686268	20.38666014608213
13	30.396914291123068	21.400242944359434	28.887504141097036	19.315338623420466
14	20.64837747874237	23.089179510640648	28.758775181813878	27.503667828803103
15	22.92700626291628	29.604978781807567	26.742968022843076	20.725046932433074
16	21.501049077915727	28.67674202148638	27.538689678019846	22.28351922257805
17	19.26627648330152	27.95847860038808	28.328258846172048	24.44698607013835
18	20.129044471438263	27.03576330278124	32.36176623704428	20.473425988736217
19	19.606240830427993	25.73490668728013	33.899256968874724	20.759595513417153
20	23.504866774992507	24.631402924797676	32.637050592374074	19.226679707835746
21	22.949723138083893	25.446686333591	31.439366451592548	20.164224076732555
22	21.35370490148133	25.21115650980454	32.286043319818894	21.149095268895234
23	20.79367082616858	26.991907113221536	32.98663806023127	19.227784000378616
24	19.343261449147327	26.37161021628358	31.536859707520232	22.748268627048855
25	20.433040432882677	27.096026124406443	32.71876824054647	19.752165202164413
26	21.478489958825662	26.299042420609254	31.457823912666232	20.76464370789885
27	20.398491851898594	27.591064695767404	31.021312846077393	20.989130606256605
28	20.231901434002744	27.04491315527931	31.29312656770102	21.430058843016926
29	19.402262222152107	27.079935004496047	32.08159144331035	21.43621133004149
30	18.940510183154807	27.267033712473772	33.771474546056886	20.020981558314535
31	20.360788149363454	26.649734181009322	32.65187966366404	20.33759800596318
32	21.138841123854295	26.97834009055199	30.95142690372147	20.931391881872248
33	21.882187761283504	27.025982425972963	30.37609048888608	20.715739323857452
34	20.499613497609996	29.26359462998312	30.444398870466483	19.7923930019404
35	19.113568600230323	30.162646515957025	30.658316111628203	20.065468772184445
36	20.127782422817837	26.844405180709586	32.573001624887596	20.454810771584974
37	20.046064774645444	27.080408272728707	31.880925712663082	20.99260123996277
38	20.347852151004115	26.818690940068468	32.292984587231224	20.540472321696193
39	21.225133698275727	26.31749988168294	32.17261670005837	20.284749719982962
40	19.59945731909322	26.989540772058245	31.519033270756758	21.891968638091782
41	20.422628531764186	26.436605720235374	31.41870040543312	21.722065342567323
42	18.228714761236176	27.876287683983026	31.79794601587026	22.097051538910538
43	18.786224739308082	27.770748868100142	31.593809651516825	21.84921674107495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	101.5
2	117.0
3	286.0
4	455.0
5	455.0
6	789.0
7	1123.0
8	1227.5
9	1332.0
10	2102.0
11	2872.0
12	2872.0
13	5046.0
14	7220.0
15	12050.5
16	16881.0
17	16167.5
18	15454.0
19	15454.0
20	16888.5
21	18323.0
22	15129.5
23	11936.0
24	13037.0
25	14138.0
26	14138.0
27	15542.0
28	16946.0
29	17904.5
30	18863.0
31	20471.0
32	22079.0
33	22079.0
34	24206.5
35	26334.0
36	28257.5
37	30181.0
38	31370.0
39	32559.0
40	32559.0
41	34088.5
42	35618.0
43	39452.0
44	43286.0
45	52495.5
46	61705.0
47	61705.0
48	61935.0
49	62165.0
50	56345.5
51	50526.0
52	46561.5
53	42597.0
54	42597.0
55	36781.0
56	30965.0
57	25492.0
58	20019.0
59	17467.5
60	14916.0
61	14916.0
62	13201.0
63	11486.0
64	9810.5
65	8135.0
66	6931.5
67	5728.0
68	5728.0
69	4754.0
70	3780.0
71	3189.5
72	2599.0
73	2076.0
74	1553.0
75	1553.0
76	1228.5
77	904.0
78	700.5
79	497.0
80	366.0
81	235.0
82	235.0
83	187.0
84	139.0
85	106.0
86	73.0
87	60.0
88	47.0
89	47.0
90	30.5
91	14.0
92	8.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	633890.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.1353150401915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.06283505324153	47.45821248898417
2	10.152307747849292	11.601106031166927
3	2.7883170957318617	4.779341270897752
4	1.1897725649317996	2.719121212942203
5	0.6398698204866488	1.8279581889107728
6	0.4247104898304913	1.4559580582403502
7	0.27739860846629855	1.109447982050289
8	0.21059852016279842	0.9626090237199687
9	0.16388279167292202	0.8427145438708625
>10	0.974512677843461	10.282971938345538
>50	0.06318601872162914	2.5194732240789546
>100	0.0439796854374877	5.60670954859541
>500	0.0038969341526877946	1.5219892885928343
>1k	0.0038969341526877946	3.916299501734435
>5k	8.350573184330988E-4	3.396087697869528
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7816	1.2330215021533704	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7286	1.14941078105034	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6252	0.986290996860654	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3284	0.5180709586836832	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2562	0.4041710706904984	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2141	0.3377557620407326	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2106	0.33223429932638154	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2009	0.31693195980375144	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1884	0.2972124501096405	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1612	0.254302797015255	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1531	0.2415245547334711	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1472	0.23221694615785074	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1388	0.21896543564340815	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1289	0.2033475839656723	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1165	0.18378583034911422	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1109	0.17495149000615248	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1073	0.1692722712142485	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	945	0.1490794932874789	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	891	0.14056066509962295	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	833	0.1314108126015555	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	737	0.11626622915647826	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	722	0.11389988799318494	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	716	0.11295335152786762	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	714	0.11263783937276184	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	673	0.10616984019309343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5775607755288772E-4	0.0
3	0.0	0.0	0.0	1.5775607755288772E-4	0.0
4	0.0	0.0	0.0	1.5775607755288772E-4	0.0
5	0.0	0.0	0.0	1.5775607755288772E-4	0.0
6	0.0	0.0	0.0	1.5775607755288772E-4	0.0
7	0.0	0.0	0.0	1.5775607755288772E-4	0.0
8	1.5775607755288772E-4	0.0	0.0	1.5775607755288772E-4	0.0
9	1.5775607755288772E-4	0.0	0.0	1.5775607755288772E-4	0.0
10	1.5775607755288772E-4	0.0	0.0	1.5775607755288772E-4	0.0
11	1.5775607755288772E-4	0.0	0.0	3.1551215510577544E-4	0.0
12	1.5775607755288772E-4	0.0	0.0	3.1551215510577544E-4	0.0
13	1.5775607755288772E-4	0.0	0.0	3.1551215510577544E-4	0.0
14	1.5775607755288772E-4	0.0	0.0	3.1551215510577544E-4	0.0
15	1.5775607755288772E-4	0.0	0.0	6.310243102115509E-4	0.0
16	1.5775607755288772E-4	0.0	0.0	0.0012620486204231018	0.0
17	1.5775607755288772E-4	0.0	0.0	0.0025240972408462035	0.0
18	1.5775607755288772E-4	0.0	0.0	0.0026818533183990915	0.0
19	1.5775607755288772E-4	0.0	0.0	0.003628389783716418	0.0
20	1.5775607755288772E-4	0.0	0.0	0.005048194481692407	0.0
21	1.5775607755288772E-4	0.0	0.0	0.007730047800091499	0.0
22	1.5775607755288772E-4	0.0	0.0	0.012304974049125242	0.0
23	1.5775607755288772E-4	0.0	0.0	0.02082380223698118	0.0
24	1.5775607755288772E-4	0.0	0.0	0.03486409313918819	0.0
25	1.5775607755288772E-4	0.0	0.0	0.04085882408619792	0.0
26	1.5775607755288772E-4	0.0	0.0	0.04921989619650097	0.0
27	1.5775607755288772E-4	0.0	0.0	0.1080629131237281	0.0
28	1.5775607755288772E-4	0.0	0.0	0.17148085629998897	0.0
29	1.5775607755288772E-4	0.0	0.0	0.24467967628452886	0.0
30	1.5775607755288772E-4	0.0	0.0	0.3817697076779883	0.0
31	1.5775607755288772E-4	0.0	0.0	0.5702882203536891	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1665	0.0	29.222221	1
CGACGAA	45	1.3222975E-4	24.666666	10
ATTGGAC	175	0.0	23.257141	3
GTATTGG	180	0.0	21.583332	1
TTGGACC	315	0.0	20.555555	4
TATACCG	45	0.0038239432	20.555555	5
GTGCGAA	45	0.0038239432	20.555555	11
ACGACGC	45	0.0038239432	20.555555	26
AGTGCGA	55	5.139598E-4	20.181818	10
GCTTAGA	55	5.139598E-4	20.181818	1
TGGACCC	350	0.0	18.5	5
CGACGCT	50	0.007031487	18.499998	27
GGACCCT	325	0.0	18.215385	6
CGTCTGT	215	0.0	18.069769	34
TATTGGA	260	0.0	17.788462	2
CTGTACT	75	2.0654239E-4	17.266666	4
GTATCAA	2900	0.0	17.096552	2
CCGAATT	65	0.0015788771	17.076923	14
GACCCTC	365	0.0	16.726027	7
TTAGAAC	90	4.440896E-5	16.444445	3
>>END_MODULE