FastQCFastQC Report
Wed 25 May 2016
SRR2088242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273188
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144631.13596735124742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT131421.0322120535223391No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104090.8175540454355524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49170.3861959113657999No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG42230.33168707213702925No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT32330.2539295060902239No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT25700.2018554997376664No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA23470.18434041162813347No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT21370.1678463824666899No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA21070.1654900925864837No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG18460.14499037062868955No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT18400.1445191126526483No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18160.14263408074848336No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16370.12857488446325288No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA15960.12535462162697103No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.12079912785857233No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT14110.11082416736569933No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13930.1094103934375756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.026.4819641
TATATCG358.8699226E-426.428575
ATGTCCG656.902875E-519.9230777
GTATTGG2150.018.9302331
ATTGGAC2550.017.4117643
GCCGTCA752.0674863E-417.26666613
TAGGTCG650.0015800617.07692321
GTATCAA57750.016.5298712
GACCCTC4200.016.2976197
TATACCG1059.3446015E-615.8571425
TTGGACC4450.015.7977534
GGACCCT4800.015.0312516
ACCCTCG4500.014.88
CGAATTA1508.1021426E-814.815
CGATCAC750.00410472814.836
TAGGAAC3900.014.70512937
GTTTAGG1405.995789E-714.5357141
AGCCGTC908.2748744E-414.38888912
CCCATAG908.2748744E-414.3888891
TATAGAT1304.444575E-614.2307692
Produced by FastQC (version 0.11.5)