##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1273188
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.816678291030076	31.0	30.0	33.0	27.0	34.0
2	30.74269628680132	31.0	31.0	34.0	27.0	34.0
3	30.684038806523468	31.0	31.0	34.0	27.0	34.0
4	34.92961369412844	35.0	35.0	37.0	32.0	37.0
5	34.856442253618475	35.0	35.0	37.0	32.0	37.0
6	34.97097129410582	35.0	35.0	37.0	32.0	37.0
7	34.540477132992145	35.0	35.0	37.0	30.0	37.0
8	34.79324498817142	35.0	35.0	37.0	32.0	37.0
9	36.24598723833401	38.0	35.0	39.0	32.0	39.0
10	36.16518613119194	38.0	35.0	39.0	32.0	39.0
11	36.266606345645734	38.0	35.0	39.0	32.0	39.0
12	36.00084041005727	38.0	35.0	39.0	30.0	39.0
13	36.184371043396574	38.0	35.0	39.0	32.0	39.0
14	37.09775461283016	39.0	36.0	40.0	31.0	41.0
15	37.15501481320905	39.0	36.0	40.0	31.0	41.0
16	37.07542169734556	39.0	36.0	40.0	31.0	41.0
17	37.1551789680707	39.0	36.0	40.0	31.0	41.0
18	37.153932490724074	39.0	36.0	40.0	31.0	41.0
19	37.23318315912497	39.0	36.0	40.0	31.0	41.0
20	37.19898632409353	39.0	36.0	40.0	31.0	41.0
21	37.13321834638718	39.0	36.0	40.0	31.0	41.0
22	37.05770475373629	39.0	36.0	40.0	31.0	41.0
23	36.93942921233942	39.0	35.0	40.0	31.0	41.0
24	36.86415046324659	39.0	35.0	40.0	31.0	41.0
25	36.78235500177507	38.0	35.0	40.0	30.0	41.0
26	36.52798172775741	38.0	35.0	40.0	30.0	41.0
27	36.393026010298556	38.0	35.0	40.0	30.0	41.0
28	36.24780393783165	38.0	35.0	40.0	30.0	41.0
29	36.08490812040327	38.0	35.0	40.0	30.0	41.0
30	35.91786209106589	38.0	34.0	40.0	29.0	41.0
31	35.68468364452068	38.0	34.0	40.0	29.0	41.0
32	35.40277083981313	38.0	34.0	40.0	27.0	41.0
33	35.55437531613556	38.0	34.0	40.0	28.0	41.0
34	35.55079061379781	38.0	34.0	40.0	28.0	41.0
35	35.480782885166995	38.0	34.0	40.0	27.0	41.0
36	35.39884840259255	38.0	34.0	40.0	27.0	41.0
37	35.23553395099545	38.0	34.0	40.0	26.0	41.0
38	35.10452737537583	38.0	34.0	40.0	26.0	41.0
39	35.077900514299536	38.0	34.0	40.0	25.0	41.0
40	34.94209181990405	38.0	33.0	40.0	25.0	41.0
41	34.79269989977914	38.0	33.0	40.0	24.0	41.0
42	34.622951205949164	38.0	33.0	40.0	24.0	41.0
43	34.262401939069484	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	4.0
16	8.0
17	42.0
18	94.0
19	249.0
20	515.0
21	1055.0
22	1751.0
23	3185.0
24	5147.0
25	8109.0
26	12227.0
27	17947.0
28	25101.0
29	33932.0
30	43306.0
31	54229.0
32	66149.0
33	80636.0
34	97060.0
35	116117.0
36	140243.0
37	173739.0
38	202443.0
39	189898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.54905952616581	25.825801060016275	13.659019720575438	21.966119693242476
2	17.907567460579273	23.71173777949525	35.422969742096214	22.95772501782926
3	19.50819517620336	26.852122388838097	32.20105750289824	21.438624932060307
4	13.499027637709435	17.16934184111066	36.14344464446727	33.18818587671263
5	11.638658234290615	40.2924784085304	34.90764914529512	13.16121421188387
6	30.911538594457376	37.268573062265745	15.868198569260786	15.951689774016092
7	24.161946232606653	35.14861905704421	22.571450563467454	18.117984146881685
8	26.64296239047179	34.42099674203652	20.653194971991567	18.282845895500113
9	25.01680820114547	14.250919738483239	24.056384445973414	36.67588761439787
10	16.146554947109145	28.815461660021928	34.50393814581979	20.53404524704914
11	31.253907514051342	23.097531550721495	24.476275302626163	21.172285632601
12	19.438684624737274	28.642667068806805	31.787685714914062	20.130962591541863
13	29.61620750431201	22.01041794299035	28.4654740698153	19.90790048288234
14	20.54480563750208	23.723519228896283	29.05918057663126	26.672494556970378
15	23.001787638589118	30.059896888754846	26.34120019981338	20.597115272842657
16	21.380738744003242	28.839652902792047	27.966490416183625	21.813117937021083
17	19.404282792486264	28.64054640791462	28.81067053726551	23.144500262333604
18	19.87876103136379	27.53261890624166	32.00171537903279	20.586904683361766
19	19.79479856863244	26.712472941937875	33.53958724084738	19.9531412485823
20	22.570193875531345	25.917146564372267	32.331753048253674	19.180906511842714
21	22.02879700405596	26.448018674382727	31.476341278742808	20.046843042818498
22	21.08478873504934	26.56716839932516	31.990326644611795	20.357716221013707
23	20.67448012390943	27.629383877322123	32.19312466030154	19.5030113384669
24	19.554221371863388	27.363908550818888	31.458354932657233	21.62351514466049
25	20.41850849992303	27.84686943326516	32.20954014646698	19.525081920344835
26	20.94286154126492	27.262902257954046	31.22806686836508	20.566169332415953
27	20.273282500306316	28.114543963656587	31.13931328287731	20.472860253159787
28	20.048963703710683	28.129310046905875	31.104440192650262	20.71728605673318
29	19.278064197903216	28.011338466903553	31.97579619035052	20.73480114484271
30	19.180827968846707	28.27752068037085	32.9250668400896	19.616584510692846
31	20.084779309889818	27.859671941614277	31.965899772853657	20.089648975642245
32	20.567818735332096	28.247045997920182	30.923712758838445	20.26142250790928
33	21.074813774556468	28.143997587159163	30.495417801612955	20.28577083667141
34	20.104179429903517	29.36424157312196	30.766391137836674	19.76518785913785
35	19.28073465976745	29.904617385649253	30.874544843338143	19.940103111245158
36	19.921252792203507	27.856294592785986	32.06839838264263	20.154054232367884
37	19.924708684027813	27.841685595528702	31.629264491968197	20.604341228475292
38	19.96123117717101	27.82801911422351	31.81274093064025	20.398008777965234
39	20.612352614067994	27.38448681577269	31.83104144870985	20.172119121449462
40	19.520762055564457	27.629226791330108	31.590621337932813	21.25938981517262
41	19.87954646132386	27.228736054691062	31.567058439130747	21.324659044854336
42	18.432784474877238	28.381590150079955	31.53894004656029	21.64668532848252
43	18.774682136495162	27.814509718910323	31.63594064662878	21.774867497965737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	237.0
1	275.5
2	314.0
3	705.0
4	1096.0
5	1096.0
6	1865.0
7	2634.0
8	2874.5
9	3115.0
10	4642.5
11	6170.0
12	6170.0
13	10696.0
14	15222.0
15	23656.5
16	32091.0
17	30974.0
18	29857.0
19	29857.0
20	33544.5
21	37232.0
22	32012.0
23	26792.0
24	30160.5
25	33529.0
26	33529.0
27	37161.0
28	40793.0
29	43014.0
30	45235.0
31	48329.5
32	51424.0
33	51424.0
34	55401.5
35	59379.0
36	63461.5
37	67544.0
38	69059.0
39	70574.0
40	70574.0
41	72755.0
42	74936.0
43	79773.5
44	84611.0
45	96077.0
46	107543.0
47	107543.0
48	107395.0
49	107247.0
50	98009.5
51	88772.0
52	81357.0
53	73942.0
54	73942.0
55	66476.5
56	59011.0
57	50814.0
58	42617.0
59	37785.5
60	32954.0
61	32954.0
62	28754.5
63	24555.0
64	21433.5
65	18312.0
66	15620.0
67	12928.0
68	12928.0
69	11044.5
70	9161.0
71	7553.0
72	5945.0
73	4657.5
74	3370.0
75	3370.0
76	2620.0
77	1870.0
78	1433.5
79	997.0
80	785.0
81	573.0
82	573.0
83	428.0
84	283.0
85	233.5
86	184.0
87	131.5
88	79.0
89	79.0
90	61.5
91	44.0
92	27.0
93	10.0
94	7.0
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1273188.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.65078012348085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.98811498435646	37.23218408962582
2	13.57894813936881	13.484107367519002
3	4.93719589521625	7.354068834598025
4	2.260463326945206	4.489350704933937
5	1.1845026025542889	2.940573913755691
6	0.7005477080049665	2.0869644129697833
7	0.4351097003476804	1.5122475243089424
8	0.32025902333128853	1.2720888279986025
9	0.2279985073349755	1.0188273380553634
>10	1.1943750352922486	10.935816334645537
>50	0.10179313082930377	3.5251074073461717
>100	0.061095761503974534	5.947946400968897
>500	0.006077583779230061	2.0768807150022446
>1k	0.0030387918896150304	3.1051566337360543
>5k	0.0	0.0
>10k+	4.79809245728689E-4	3.01867949453603
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14463	1.13596735124742	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13142	1.0322120535223391	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10409	0.8175540454355524	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4917	0.3861959113657999	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4223	0.33168707213702925	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3233	0.2539295060902239	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2570	0.2018554997376664	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2347	0.18434041162813347	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2137	0.1678463824666899	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2107	0.1654900925864837	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1846	0.14499037062868955	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1840	0.1445191126526483	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1816	0.14263408074848336	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1637	0.12857488446325288	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1596	0.12535462162697103	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1538	0.12079912785857233	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1411	0.11082416736569933	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1393	0.1094103934375756	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.5708599201374817E-4	0.0
11	0.0	0.0	0.0	2.3562898802062225E-4	0.0
12	0.0	0.0	0.0	2.3562898802062225E-4	0.0
13	0.0	0.0	0.0	2.3562898802062225E-4	0.0
14	0.0	0.0	0.0	3.1417198402749634E-4	0.0
15	0.0	0.0	0.0	5.498009720481186E-4	0.0
16	0.0	0.0	0.0	0.0012566879361099854	0.0
17	0.0	0.0	0.0	0.0015708599201374816	0.0
18	0.0	0.0	0.0	0.0017279459121512298	0.0
19	0.0	0.0	0.0	0.0023562898802062227	0.0
20	0.0	0.0	0.0	0.002906090852254341	0.0
21	0.0	0.0	0.0	0.003691520812323082	0.0
22	0.0	0.0	0.0	0.005419466724474312	0.0
23	0.0	0.0	0.0	0.0084826435687424	0.0
24	0.0	0.0	0.0	0.015080255233319824	0.0
25	0.0	0.0	0.0	0.021128065925849128	0.0
26	0.0	0.0	0.0	0.02984633848261215	0.0
27	0.0	0.0	0.0	0.08953901544783645	0.0
28	0.0	0.0	0.0	0.15630056205367943	0.0
29	0.0	0.0	0.0	0.21984184582324057	0.0
30	0.0	0.0	0.0	0.33082309918095365	0.0
31	0.0	0.0	0.0	0.5051100073202073	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3465	0.0	26.481964	1
TATATCG	35	8.8699226E-4	26.42857	5
ATGTCCG	65	6.902875E-5	19.923077	7
GTATTGG	215	0.0	18.930233	1
ATTGGAC	255	0.0	17.411764	3
GCCGTCA	75	2.0674863E-4	17.266666	13
TAGGTCG	65	0.00158006	17.076923	21
GTATCAA	5775	0.0	16.529871	2
GACCCTC	420	0.0	16.297619	7
TATACCG	105	9.3446015E-6	15.857142	5
TTGGACC	445	0.0	15.797753	4
GGACCCT	480	0.0	15.031251	6
ACCCTCG	450	0.0	14.8	8
CGAATTA	150	8.1021426E-8	14.8	15
CGATCAC	75	0.004104728	14.8	36
TAGGAAC	390	0.0	14.705129	37
GTTTAGG	140	5.995789E-7	14.535714	1
AGCCGTC	90	8.2748744E-4	14.388889	12
CCCATAG	90	8.2748744E-4	14.388889	1
TATAGAT	130	4.444575E-6	14.230769	2
>>END_MODULE