FastQCFastQC Report
Wed 25 May 2016
SRR2088241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113943
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323271.038137178490422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT294210.9448149821624866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT259510.833380700931263No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG166820.5357195041784644No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115690.3715225358974137No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT97790.3140391458674741No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA96040.3084192613673404No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA88550.28436615570676793No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT86870.2789710665866395No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA70690.2270112201796886No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG66100.2122710659764806No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT64670.20767881749922845No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT61730.19823741153900376No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52030.16708719459540525No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA51490.16535305880679255No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA48800.15671449348944408No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT41500.13327154671745758No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38720.12434395876867368No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC36450.11705416573135731No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA36010.1156411661998951No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG34480.11072778146549248No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC33270.10684203275397142No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33250.10677780550254132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.029.7736361
ATTGGAC10550.019.4644553
GTATTGG10550.019.28911
TAATCCG500.007037516718.55
GTATCAA123300.018.1549072
TTGGACC17750.017.7183114
TATTGGA12650.017.4031622
TTAGTAC1401.873559E-917.1785723
GACCCTC18450.016.3441737
GGACCCT18450.016.2439026
TGGACCC18850.016.0954915
CGTCTGT7050.015.48226834
ACCCTCG20900.014.693788
CCCTCGC20850.014.3741019
AGTATCA5100.014.14705851
CGGGATA2009.858923E-1013.87500124
TATACCG2800.013.8755
TGCGACG1203.3039225E-513.87499922
TAAACGT2750.013.4545464
CGATGCG1652.5991903E-713.45454533
Produced by FastQC (version 0.11.5)