##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088241_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3113943
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.84188856379195	31.0	30.0	33.0	27.0	34.0
2	30.72483247124305	31.0	31.0	34.0	27.0	34.0
3	30.681884029348	31.0	31.0	34.0	27.0	34.0
4	34.909929308275714	35.0	35.0	37.0	32.0	37.0
5	34.82833404465014	35.0	35.0	37.0	32.0	37.0
6	34.94121536585609	35.0	35.0	37.0	32.0	37.0
7	34.52304554065376	35.0	35.0	37.0	30.0	37.0
8	34.77727819680707	35.0	35.0	37.0	32.0	37.0
9	36.22361616766909	38.0	35.0	39.0	32.0	39.0
10	36.13928096949751	38.0	35.0	39.0	31.0	39.0
11	36.22455260099495	38.0	35.0	39.0	32.0	39.0
12	35.95394520708953	37.0	35.0	39.0	30.0	39.0
13	36.13172688132057	38.0	35.0	39.0	31.0	39.0
14	37.057789432883006	39.0	36.0	40.0	31.0	41.0
15	37.103823673073016	39.0	36.0	40.0	31.0	41.0
16	37.036927779345994	39.0	36.0	40.0	31.0	41.0
17	37.10581471786735	39.0	36.0	40.0	31.0	41.0
18	37.0965383759433	39.0	36.0	40.0	31.0	41.0
19	37.167448151748445	39.0	36.0	40.0	31.0	41.0
20	37.11742154560954	39.0	36.0	40.0	31.0	41.0
21	37.05601098029091	39.0	36.0	40.0	31.0	41.0
22	36.9742435876315	39.0	36.0	40.0	31.0	41.0
23	36.85981342625732	39.0	35.0	40.0	31.0	41.0
24	36.782440783277025	39.0	35.0	40.0	30.0	41.0
25	36.70563398238182	38.0	35.0	40.0	30.0	41.0
26	36.44094095492435	38.0	35.0	40.0	30.0	41.0
27	36.3077413427285	38.0	35.0	40.0	30.0	41.0
28	36.14885885836703	38.0	35.0	40.0	30.0	41.0
29	35.98061942688097	38.0	34.0	40.0	29.0	41.0
30	35.8342647890472	38.0	34.0	40.0	29.0	41.0
31	35.60736500314874	38.0	34.0	40.0	27.0	41.0
32	35.367491954733914	38.0	34.0	40.0	27.0	41.0
33	35.53456277138021	38.0	34.0	40.0	27.0	41.0
34	35.54163804539775	38.0	34.0	40.0	27.0	41.0
35	35.47727077855953	38.0	34.0	40.0	27.0	41.0
36	35.3992253551205	38.0	34.0	40.0	27.0	41.0
37	35.25410612846799	38.0	34.0	40.0	26.0	41.0
38	35.140499681593404	38.0	34.0	40.0	26.0	41.0
39	35.12121641276028	38.0	34.0	40.0	25.0	41.0
40	35.00117343188363	38.0	33.0	40.0	25.0	41.0
41	34.85928547825056	38.0	33.0	40.0	24.0	41.0
42	34.684291909004116	38.0	33.0	40.0	24.0	41.0
43	34.35810482080115	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	6.0
15	7.0
16	28.0
17	112.0
18	253.0
19	594.0
20	1219.0
21	2550.0
22	4557.0
23	7978.0
24	13143.0
25	20618.0
26	31311.0
27	45186.0
28	62989.0
29	83721.0
30	107850.0
31	133639.0
32	161861.0
33	196247.0
34	235372.0
35	281709.0
36	342555.0
37	424165.0
38	503666.0
39	452606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.159804787692	25.203736869942706	13.269671281715818	22.36678706064947
2	18.126343353105693	23.380100406462162	35.39650533102244	23.09705090940971
3	19.882926566093214	26.91414068915198	31.626622581081286	21.576310163673515
4	13.47006030617773	16.344679398434717	35.76401366370547	34.42124663168208
5	11.701948301558506	40.21746705061718	34.84575665000933	13.234827997814987
6	31.84159119161783	36.510655461580384	15.56258415777039	16.085169189031397
7	24.77636873892682	34.70143801604589	22.076993702196862	18.445199542830423
8	27.271372661606204	34.108556258094644	20.392537692565345	18.227533387733814
9	25.211090890231453	13.854042928852584	23.31102399754909	37.623842183366875
10	15.978487724406001	28.00889418977804	34.29279212882188	21.719825956994075
11	32.05864718782585	21.965463080088494	24.808996182653313	21.166893549432345
12	19.979042647858357	27.229239584668058	32.06201911852593	20.729698648947654
13	30.44663951780749	20.992227539168187	28.575314320140095	19.98581862288423
14	20.83715084059021	22.498388698829746	28.445350476871283	28.219109983708755
15	23.07213073585483	29.237657850513	26.3391141070983	21.35109730653387
16	22.057372276884966	28.51503704467294	26.958489606264468	22.469101072177622
17	19.831673219451996	27.373879354888643	28.037314748535856	24.757132677123504
18	20.873407124022503	26.48664410363324	31.686193356782706	20.953755415561556
19	20.218160704932618	25.372301291321005	32.729404488136105	21.680133515610272
20	23.866204358910874	24.539498635652613	31.5797045739116	20.014592431524918
21	23.35993304951311	25.246480105769436	30.5420490998069	20.85153774491055
22	22.011578246615304	25.002255982206485	31.41509012849625	21.571075642681965
23	21.273928263940604	26.562785510203625	32.142367410064985	20.020918815790782
24	20.19908521125788	25.85323495002959	30.870410922743286	23.077268915969242
25	21.282727397386527	26.565065577629394	31.698814011688718	20.45339301329536
26	22.270960001515764	25.766367592470385	30.597188195159642	21.365484210854213
27	21.19598849433018	26.96086601456738	30.330034942836143	21.513110548266297
28	21.041200818383636	26.535970632731555	30.45312647020193	21.96970207868288
29	20.237525221238794	26.429578190737598	31.246268798112233	22.086627789911375
30	19.761793969896043	26.677077904123486	32.75451734344527	20.8066107825352
31	20.9717711595877	26.190363792786187	31.77562980439912	21.062235243226997
32	21.786782866609954	26.385261387250825	30.12068621679973	21.70726952933949
33	22.508022786544263	26.31133582085478	29.83770094699871	21.34294044560225
34	21.155172076046348	28.46317353914314	29.872190981016672	20.50946340379384
35	20.091761474118183	29.291672968965717	29.976463923713442	20.64010163320266
36	20.919811313180748	26.459090612769725	31.631792874821407	20.989305199228117
37	20.91300322452916	26.60556085965607	30.990002064906136	21.49143385090864
38	21.1717105932896	26.225977803704176	31.391743522601413	21.210568080404812
39	21.902263464681273	25.779469951762124	31.32873658894848	20.989529994608123
40	20.496264703624952	26.42935339535759	30.730106491994235	22.344275409023222
41	21.315322727487303	25.946492919106095	30.66979710290137	22.06838725050523
42	19.250191798629583	27.489199384831387	30.911709045412845	22.34889977112619
43	19.819148905423127	27.12059918887404	30.63354081946908	22.426711086233755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	304.0
1	428.0
2	552.0
3	1286.0
4	2020.0
5	2020.0
6	3526.5
7	5033.0
8	5446.5
9	5860.0
10	9034.0
11	12208.0
12	12208.0
13	21612.5
14	31017.0
15	49882.5
16	68748.0
17	67400.0
18	66052.0
19	66052.0
20	72255.5
21	78459.0
22	66860.0
23	55261.0
24	61298.0
25	67335.0
26	67335.0
27	74360.5
28	81386.0
29	86593.0
30	91800.0
31	98883.5
32	105967.0
33	105967.0
34	115436.0
35	124905.0
36	134874.5
37	144844.0
38	150210.5
39	155577.0
40	155577.0
41	163528.5
42	171480.0
43	188654.5
44	205829.0
45	246158.5
46	286488.0
47	286488.0
48	290529.0
49	294570.0
50	272202.5
51	249835.0
52	233713.5
53	217592.0
54	217592.0
55	190599.0
56	163606.0
57	139538.5
58	115471.0
59	102998.0
60	90525.0
61	90525.0
62	79972.5
63	69420.0
64	60688.0
65	51956.0
66	43812.5
67	35669.0
68	35669.0
69	30415.0
70	25161.0
71	21074.0
72	16987.0
73	13385.0
74	9783.0
75	9783.0
76	7646.0
77	5509.0
78	4225.0
79	2941.0
80	2340.0
81	1739.0
82	1739.0
83	1300.0
84	861.0
85	720.0
86	579.0
87	454.0
88	329.0
89	329.0
90	273.0
91	217.0
92	132.0
93	47.0
94	31.5
95	16.0
96	16.0
97	10.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3113943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.11502332703306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.62907267000884	32.04033235058219
2	14.44377710766921	12.743751280705865
3	5.459344572213682	7.225173394605537
4	2.583152758781148	4.558233768436805
5	1.328943392872708	2.931318438844298
6	0.8047023234153905	2.129967706127259
7	0.5183934826711231	1.6008258406432954
8	0.37393015900540233	1.3196750149763603
9	0.2431144509558595	0.9652499707550918
>10	1.3881061525485723	11.414686693365224
>50	0.1319304685841078	4.094882524922121
>100	0.08417895612207225	6.962870017479301
>500	0.005381856338305558	1.6608392302196247
>1k	0.004865787922241435	4.187516042879263
>5k	7.372405942790052E-4	2.3935911171050397
>10k+	3.686202971395026E-4	3.7710866083527548
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32327	1.038137178490422	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29421	0.9448149821624866	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25951	0.833380700931263	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16682	0.5357195041784644	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11569	0.3715225358974137	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9779	0.3140391458674741	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9604	0.3084192613673404	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8855	0.28436615570676793	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8687	0.2789710665866395	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7069	0.2270112201796886	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6610	0.2122710659764806	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6467	0.20767881749922845	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6173	0.19823741153900376	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5203	0.16708719459540525	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5149	0.16535305880679255	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4880	0.15671449348944408	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4150	0.13327154671745758	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3872	0.12434395876867368	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3645	0.11705416573135731	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3601	0.1156411661998951	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3448	0.11072778146549248	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3327	0.10684203275397142	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3325	0.10677780550254132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.211362571505002E-5	0.0
5	0.0	0.0	0.0	6.422725143010004E-5	0.0
6	0.0	0.0	0.0	6.422725143010004E-5	0.0
7	0.0	0.0	0.0	9.634087714515005E-5	0.0
8	0.0	0.0	0.0	9.634087714515005E-5	0.0
9	0.0	0.0	0.0	2.5690900572040016E-4	0.0
10	0.0	0.0	0.0	2.8902263143545015E-4	0.0
11	0.0	0.0	0.0	4.1747713429565026E-4	0.0
12	6.422725143010004E-5	0.0	0.0	4.817043857257503E-4	0.0
13	6.422725143010004E-5	0.0	0.0	5.138180114408003E-4	0.0
14	6.422725143010004E-5	0.0	0.0	6.422725143010004E-4	0.0
15	6.422725143010004E-5	0.0	0.0	8.028406428762505E-4	0.0
16	6.422725143010004E-5	0.0	0.0	0.001412999531462201	0.0
17	6.422725143010004E-5	0.0	0.0	0.0022800674257685513	0.0
18	6.422725143010004E-5	0.0	0.0	0.0026654309343491518	0.0
19	6.422725143010004E-5	0.0	0.0	0.003147135320074902	0.0
20	6.422725143010004E-5	0.0	0.0	0.003757294208660852	0.0
21	6.422725143010004E-5	0.0	0.0	0.005395089120128403	0.0
22	6.422725143010004E-5	0.0	0.0	0.008831247071638754	0.0
23	6.422725143010004E-5	0.0	0.0	0.013937313560331708	0.0
24	6.422725143010004E-5	0.0	0.0	0.023121810514836014	0.0
25	6.422725143010004E-5	0.0	0.0	0.027585604489227967	0.0
26	6.422725143010004E-5	0.0	0.0	0.03484328390082927	0.0
27	6.422725143010004E-5	0.0	0.0	0.08741328919636615	0.0
28	6.422725143010004E-5	0.0	0.0	0.13510202338321542	0.0
29	6.422725143010004E-5	0.0	0.0	0.19473702633606332	0.0
30	9.634087714515005E-5	0.0	0.0	0.3041802627729538	0.0
31	9.634087714515005E-5	0.0	0.0	0.4586146888366293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7245	0.0	29.773636	1
ATTGGAC	1055	0.0	19.464455	3
GTATTGG	1055	0.0	19.2891	1
TAATCCG	50	0.0070375167	18.5	5
GTATCAA	12330	0.0	18.154907	2
TTGGACC	1775	0.0	17.718311	4
TATTGGA	1265	0.0	17.403162	2
TTAGTAC	140	1.873559E-9	17.178572	3
GACCCTC	1845	0.0	16.344173	7
GGACCCT	1845	0.0	16.243902	6
TGGACCC	1885	0.0	16.095491	5
CGTCTGT	705	0.0	15.482268	34
ACCCTCG	2090	0.0	14.69378	8
CCCTCGC	2085	0.0	14.374101	9
AGTATCA	510	0.0	14.1470585	1
CGGGATA	200	9.858923E-10	13.875001	24
TATACCG	280	0.0	13.875	5
TGCGACG	120	3.3039225E-5	13.874999	22
TAAACGT	275	0.0	13.454546	4
CGATGCG	165	2.5991903E-7	13.454545	33
>>END_MODULE