FastQCFastQC Report
Wed 25 May 2016
SRR2088240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541358
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT298731.175473900174631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT269451.0602599082852553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT236150.9292276019356581No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG144610.5690264811175757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108010.4250089912558561No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA88920.34989167209027616No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT86490.3403298551404406No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA84870.333955310507217No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT82090.32301627712427766No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA64140.2523847486265217No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT60150.23668448128913752No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG58580.23050668186064302No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT55350.21779694163514152No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47690.18765557627063956No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47020.18501919052726926No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46800.18415351162646112No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT38070.14980179888075587No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37420.14724411121927725No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA34420.13543939893552975No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC33020.12993053320311423No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC32920.129537042793656No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG30250.1190308488611207No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG29730.11698469873193781No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28120.11064950313965999No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26350.10368472289224895No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC26140.10285839303238661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65650.029.1378541
ATTGGAC10850.018.9262683
CGATCGA609.2410133E-418.51
GTATTGG10700.018.3271031
TTGGACC16000.017.3437484
GTATCAA115050.017.1251622
TATTGGA12850.016.2684822
GATCGAT700.00259365215.85714326
CGTCTGT7000.015.85714234
GGACCCT16850.015.7002976
TGGACCC17600.015.2414775
AACAACG3050.015.1639352
TATACTG4350.014.459775
TGCGTCT7750.014.0838732
GGCGTAA800.006301388613.87500113
GGGTAGG4050.013.7037041
CGGACCA1102.4581954E-413.4545459
GACCCTC19750.013.39493757
CGTTGAT23700.013.11392434
TCTAACG1153.5798238E-412.8695642
Produced by FastQC (version 0.11.5)