##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088240_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2541358
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.80808803797025	31.0	30.0	33.0	27.0	34.0
2	30.67614401434194	31.0	31.0	34.0	27.0	34.0
3	30.63354552959481	31.0	30.0	34.0	27.0	34.0
4	34.857470690866855	35.0	35.0	37.0	32.0	37.0
5	34.76507481433155	35.0	35.0	37.0	32.0	37.0
6	34.877463545081014	35.0	35.0	37.0	32.0	37.0
7	34.44939162447794	35.0	35.0	37.0	30.0	37.0
8	34.70926646304849	35.0	35.0	37.0	31.0	37.0
9	36.12550770100081	38.0	35.0	39.0	31.0	39.0
10	36.0406998935215	37.0	35.0	39.0	30.0	39.0
11	36.13874235743252	38.0	35.0	39.0	31.0	39.0
12	35.86515516507316	37.0	35.0	39.0	30.0	39.0
13	36.04710237597379	37.0	35.0	39.0	31.0	39.0
14	36.9357028801137	38.0	36.0	40.0	31.0	41.0
15	36.98752832147222	38.0	36.0	40.0	31.0	41.0
16	36.904932717074885	38.0	36.0	40.0	31.0	41.0
17	36.98835268388004	38.0	36.0	40.0	31.0	41.0
18	36.98967284420377	38.0	36.0	40.0	31.0	41.0
19	37.06731125642275	39.0	36.0	40.0	31.0	41.0
20	37.02746326963773	39.0	36.0	40.0	31.0	41.0
21	36.96369106595765	39.0	36.0	40.0	31.0	41.0
22	36.88244355970312	39.0	36.0	40.0	31.0	41.0
23	36.75527336172235	38.0	35.0	40.0	30.0	41.0
24	36.68012967869934	38.0	35.0	40.0	30.0	41.0
25	36.593700297242655	38.0	35.0	40.0	30.0	41.0
26	36.32440333081762	38.0	35.0	40.0	30.0	41.0
27	36.17657803426357	38.0	35.0	40.0	30.0	41.0
28	36.01600955079922	38.0	34.0	40.0	29.0	41.0
29	35.8451375209632	38.0	34.0	40.0	29.0	41.0
30	35.67961735418623	38.0	34.0	40.0	28.0	41.0
31	35.42829030778033	38.0	34.0	40.0	27.0	41.0
32	35.18252091991762	37.0	34.0	40.0	27.0	41.0
33	35.34104246627197	38.0	34.0	40.0	27.0	41.0
34	35.3354816598055	38.0	34.0	40.0	27.0	41.0
35	35.257013376312976	38.0	34.0	40.0	27.0	41.0
36	35.1705009683799	38.0	34.0	40.0	26.0	41.0
37	35.016623789328385	38.0	33.0	40.0	25.0	41.0
38	34.892345745857135	38.0	33.0	40.0	25.0	41.0
39	34.86788087313948	38.0	33.0	40.0	25.0	41.0
40	34.72746932939003	38.0	33.0	40.0	24.0	41.0
41	34.57264029703804	38.0	33.0	40.0	24.0	41.0
42	34.38026952519086	38.0	33.0	40.0	23.0	41.0
43	34.0353917078979	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	6.0
15	18.0
16	46.0
17	87.0
18	231.0
19	534.0
20	1164.0
21	2299.0
22	4049.0
23	7030.0
24	11764.0
25	18250.0
26	27049.0
27	39447.0
28	54283.0
29	71953.0
30	92505.0
31	113486.0
32	137377.0
33	165409.0
34	197481.0
35	233707.0
36	280888.0
37	345972.0
38	399497.0
39	336821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.18676550096444	25.421683997295936	13.46142495468958	21.930125547050043
2	18.336495684590677	23.34842237890136	35.38722210723558	22.927859829272382
3	20.130654555556518	26.878188747905646	31.6490632173822	21.342093479155633
4	13.583485679703527	16.332330982096973	35.274054265475385	34.81012907272411
5	11.45682741274547	40.501259562800676	34.66296365958673	13.378949364867129
6	31.887597103595795	36.35430348656112	15.284426672668708	16.47367273717438
7	24.484743983334894	35.041855574854075	21.82478816443807	18.648612277372965
8	27.438676487137982	34.10310550500953	20.208801750874926	18.249416256977568
9	25.04763201406492	13.832801203136274	23.44226984155715	37.67729694124165
10	16.23415512493714	27.94675130383047	33.90010380277001	21.918989768462374
11	31.64020968316939	21.874486003152647	24.89483968807228	21.59046462560568
12	19.921357006765675	27.414594874079135	31.940915054077383	20.723133065077807
13	30.807938118124245	21.047998747126538	28.683483397459153	19.46057973729006
14	20.75957027699364	22.665362377122783	28.32273139006783	28.25233595581575
15	23.088246520167562	29.1203364500397	26.626315536811422	21.16510149298131
16	21.817272497617417	28.559651965602644	27.22658515643998	22.39649038033996
17	19.515825790778003	27.295760770422746	28.098402507635683	25.090010931163576
18	20.68567277809738	26.46687322289894	32.047944445450035	20.79950955355365
19	20.07910731191749	25.05896453785732	33.23467217133517	21.627255978890027
20	24.067762196431985	24.17620815327868	32.15218005491552	19.603849595373813
21	23.422280528756673	24.992267913454143	30.893837074508983	20.691614483280198
22	21.947203030820532	24.54498736502295	31.826173250679364	21.681636353477156
23	21.02147749352905	26.5845662043679	32.68831073780239	19.70564556430066
24	19.965585328788784	25.67194389771138	31.194581794457925	23.167888979041916
25	20.924167315270026	26.563317722257157	32.141044276327854	20.371470686144967
26	22.231499851654117	25.591357061854332	30.98284460512844	21.194298481363113
27	21.072434501553893	26.876850880513487	30.593328448805718	21.4573861691269
28	20.799312808348922	26.436613810411597	30.790349096821462	21.97372428441802
29	20.001943842622723	26.340523452421895	31.666573540603093	21.990959164352287
30	19.38046508992436	26.632453987198968	33.29928329656821	20.687797626308456
31	20.604298961421414	26.023527578562327	32.38123082226117	20.99094263775509
32	21.566501059669672	26.277132147458172	30.45812514411586	21.698241648756294
33	22.445716030563187	26.189659229435602	30.104416615053843	21.260208124947372
34	20.902525342749822	28.598056629565765	30.151241973779374	20.348176053905036
35	19.67581899126373	29.553569390853234	30.334529806505028	20.436081811378013
36	20.63750955197969	26.148893623015727	32.24484704634294	20.968749778661643
37	20.636998014447393	26.472145994385677	31.40938820898118	21.481467782185746
38	20.869314752191546	26.01581516653695	31.999505776045723	21.11536430522579
39	21.691827755082123	25.573689342469656	31.97644723805147	20.75803566439675
40	20.236778918987408	26.316559886485884	31.06465126125481	22.382009933271895
41	21.071883614980653	25.927437220572624	30.9031234481722	22.097555716274528
42	18.933420635738845	27.409991036288474	31.29885675296436	22.357731575008323
43	19.462822632623975	27.173542649245007	30.921302705089172	22.442332013041845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	284.0
2	372.0
3	961.5
4	1551.0
5	1551.0
6	2778.0
7	4005.0
8	4553.0
9	5101.0
10	7929.5
11	10758.0
12	10758.0
13	19706.0
14	28654.0
15	45638.5
16	62623.0
17	60752.5
18	58882.0
19	58882.0
20	63830.5
21	68779.0
22	56823.5
23	44868.0
24	49133.5
25	53399.0
26	53399.0
27	58743.5
28	64088.0
29	68463.0
30	72838.0
31	77613.0
32	82388.0
33	82388.0
34	90045.5
35	97703.0
36	105123.0
37	112543.0
38	117276.5
39	122010.0
40	122010.0
41	127689.5
42	133369.0
43	149936.5
44	166504.0
45	204848.5
46	243193.0
47	243193.0
48	247681.0
49	252169.0
50	230691.5
51	209214.0
52	195979.0
53	182744.0
54	182744.0
55	158704.5
56	134665.0
57	111719.0
58	88773.0
59	78395.5
60	68018.0
61	68018.0
62	60265.0
63	52512.0
64	45953.5
65	39395.0
66	33866.5
67	28338.0
68	28338.0
69	23977.5
70	19617.0
71	16538.5
72	13460.0
73	10936.5
74	8413.0
75	8413.0
76	6470.0
77	4527.0
78	3550.0
79	2573.0
80	2010.0
81	1447.0
82	1447.0
83	1113.0
84	779.0
85	594.0
86	409.0
87	334.5
88	260.0
89	260.0
90	208.5
91	157.0
92	102.5
93	48.0
94	30.0
95	12.0
96	12.0
97	7.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2541358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.39812276852764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.04116183382399	34.81769039458717
2	13.68686440147984	12.700896296321046
3	4.923033075003112	6.852584790225497
4	2.1432131476490968	3.9776426697498146
5	1.1311349473720045	2.624126907796916
6	0.6840882798571201	1.9044247195992934
7	0.43986195057847294	1.428613814890082
8	0.3032869969938242	1.1257557856494027
9	0.20687249970306487	0.8638646074789501
>10	1.2428140683269229	11.08564670144896
>50	0.12201363543492527	3.913756013813859
>100	0.06400922935400732	5.579709403651583
>500	0.005052280374915026	1.6703553768431783
>1k	0.0054804397287212415	4.948727280686026
>5k	6.850549660901552E-4	2.306775833689404
>10k+	4.2815935380634696E-4	4.19942940356881
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29873	1.175473900174631	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26945	1.0602599082852553	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23615	0.9292276019356581	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14461	0.5690264811175757	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10801	0.4250089912558561	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8892	0.34989167209027616	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8649	0.3403298551404406	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8487	0.333955310507217	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8209	0.32301627712427766	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6414	0.2523847486265217	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6015	0.23668448128913752	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5858	0.23050668186064302	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5535	0.21779694163514152	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4769	0.18765557627063956	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4702	0.18501919052726926	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4680	0.18415351162646112	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3807	0.14980179888075587	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3742	0.14724411121927725	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3442	0.13543939893552975	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3302	0.12993053320311423	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3292	0.129537042793656	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3025	0.1190308488611207	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2973	0.11698469873193781	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2812	0.11064950313965999	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2635	0.10368472289224895	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2614	0.10285839303238661	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.934904094582503E-5	0.0	0.0	0.0	0.0
7	3.934904094582503E-5	0.0	0.0	0.0	0.0
8	3.934904094582503E-5	0.0	0.0	0.0	0.0
9	3.934904094582503E-5	0.0	0.0	2.3609424567495017E-4	0.0
10	3.934904094582503E-5	0.0	0.0	3.1479232756660025E-4	0.0
11	3.934904094582503E-5	0.0	0.0	5.115375322957254E-4	0.0
12	3.934904094582503E-5	0.0	0.0	6.295846551332005E-4	0.0
13	3.934904094582503E-5	0.0	0.0	7.869808189165005E-4	0.0
14	3.934904094582503E-5	0.0	0.0	0.0011017731464831007	0.0
15	3.934904094582503E-5	0.0	0.0	0.001534612596887176	0.0
16	3.934904094582503E-5	0.0	0.0	0.0022428953339120265	0.0
17	3.934904094582503E-5	0.0	0.0	0.0038955550536366778	0.0
18	3.934904094582503E-5	0.0	0.0	0.004446441626878228	0.0
19	3.934904094582503E-5	0.0	0.0	0.005430167650523854	0.0
20	3.934904094582503E-5	0.0	0.0	0.006925431206465204	0.0
21	3.934904094582503E-5	0.0	0.0	0.009286373663214706	0.0
22	3.934904094582503E-5	0.0	0.0	0.014441098027117785	0.0
23	3.934904094582503E-5	0.0	0.0	0.02522273524627384	0.0
24	3.934904094582503E-5	0.0	0.0	0.0393096919048792	0.0
25	3.934904094582503E-5	0.0	0.0	0.04466116147351141	0.0
26	3.934904094582503E-5	0.0	0.0	0.05367209185010534	0.0
27	3.934904094582503E-5	0.0	0.0	0.11545008613505063	0.0
28	3.934904094582503E-5	0.0	0.0	0.17714938233810426	0.0
29	3.934904094582503E-5	0.0	0.0	0.24785960891775186	0.0
30	3.934904094582503E-5	0.0	0.0	0.38105611251936955	0.0
31	3.934904094582503E-5	0.0	0.0	0.5729220361712124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6565	0.0	29.137854	1
ATTGGAC	1085	0.0	18.926268	3
CGATCGA	60	9.2410133E-4	18.5	1
GTATTGG	1070	0.0	18.327103	1
TTGGACC	1600	0.0	17.343748	4
GTATCAA	11505	0.0	17.125162	2
TATTGGA	1285	0.0	16.268482	2
GATCGAT	70	0.002593652	15.857143	26
CGTCTGT	700	0.0	15.857142	34
GGACCCT	1685	0.0	15.700297	6
TGGACCC	1760	0.0	15.241477	5
AACAACG	305	0.0	15.163935	2
TATACTG	435	0.0	14.45977	5
TGCGTCT	775	0.0	14.08387	32
GGCGTAA	80	0.0063013886	13.875001	13
GGGTAGG	405	0.0	13.703704	1
CGGACCA	110	2.4581954E-4	13.454545	9
GACCCTC	1975	0.0	13.3949375	7
CGTTGAT	2370	0.0	13.113924	34
TCTAACG	115	3.5798238E-4	12.869564	2
>>END_MODULE