FastQCFastQC Report
Wed 25 May 2016
SRR2088239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325356
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT269301.1581022432694177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT240591.0346372770448913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT213760.919257094397589No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG153140.6585658282000691No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91050.3915529493118473No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA90810.3905208492807123No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA86660.37267411957566926No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT84210.36213809842449934No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT78240.3364646101500157No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA60030.2581540202876463No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG59990.25798200361579043No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT57990.24938117002299862No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT54900.23609288212213525No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA50760.21828915658505624No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47720.20521588952401265No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA46620.20048543104797717No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT35430.15236376709630697No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA32360.13916148753137153No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC32160.13830140417209236No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31730.1364522249496421No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30990.13326991652030915No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG30570.13146374146582288No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC29830.1282814330364899No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT26670.11469211595987884No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC23960.10303798644164593No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA23780.10226391141829466No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA23570.10136082389105151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62450.028.971981
GTATTGG8050.020.2236021
ATTGGAC8750.019.0285723
TTGGACC14100.018.54
GTATCAA105450.017.8421062
TGGACCC14250.017.7859655
TCTAACG852.7250571E-517.4117642
GGACCCT14850.017.191926
AAGACCG1655.4569682E-1116.8181825
AATGCGT904.4491062E-516.44444535
CGATCGA700.002593563915.8571421
ACAGCGT1302.5920963E-715.6538468
AGTATCA4500.015.6222221
TATTGGA10250.015.5219512
CGTCTGT5250.015.50476234
GACCCTC16900.014.9970417
ATTAGAC2109.094947E-1214.976193
TATACCG1508.111965E-814.85
TATACTG2500.014.7999995
GACCGTG1803.3360266E-914.3888887
Produced by FastQC (version 0.11.5)