##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2325356
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.954190240118074	31.0	30.0	33.0	27.0	34.0
2	30.803469232237987	31.0	31.0	34.0	27.0	34.0
3	30.693623255965967	31.0	31.0	34.0	27.0	34.0
4	34.93386259996319	35.0	35.0	37.0	32.0	37.0
5	34.87174307933925	35.0	35.0	37.0	32.0	37.0
6	34.98598580174391	35.0	35.0	37.0	32.0	37.0
7	34.55529518920974	35.0	35.0	37.0	30.0	37.0
8	34.82330232446129	35.0	35.0	37.0	32.0	37.0
9	36.256869055748886	38.0	35.0	39.0	32.0	39.0
10	36.18707329114338	38.0	35.0	39.0	32.0	39.0
11	36.279458715138674	38.0	35.0	39.0	32.0	39.0
12	36.00765603202262	38.0	35.0	39.0	30.0	39.0
13	36.18596550377663	38.0	35.0	39.0	32.0	39.0
14	37.10998014927607	39.0	36.0	40.0	31.0	41.0
15	37.157095085655705	39.0	36.0	40.0	31.0	41.0
16	37.071283278775375	39.0	36.0	40.0	31.0	41.0
17	37.15777885192633	39.0	36.0	40.0	31.0	41.0
18	37.15047803433109	39.0	36.0	40.0	31.0	41.0
19	37.23042751303456	39.0	36.0	40.0	31.0	41.0
20	37.17948563574782	39.0	36.0	40.0	31.0	41.0
21	37.114259494030165	39.0	36.0	40.0	31.0	41.0
22	37.02931121084256	39.0	36.0	40.0	31.0	41.0
23	36.91080634535099	39.0	35.0	40.0	31.0	41.0
24	36.83563462970831	39.0	35.0	40.0	31.0	41.0
25	36.7593779189079	38.0	35.0	40.0	30.0	41.0
26	36.49424991270154	38.0	35.0	40.0	30.0	41.0
27	36.35391355130139	38.0	35.0	40.0	30.0	41.0
28	36.197078640861875	38.0	35.0	40.0	30.0	41.0
29	36.031454968615556	38.0	34.0	40.0	29.0	41.0
30	35.87248275102823	38.0	34.0	40.0	29.0	41.0
31	35.62371654060711	38.0	34.0	40.0	28.0	41.0
32	35.36519182439162	38.0	34.0	40.0	27.0	41.0
33	35.53793655681108	38.0	34.0	40.0	28.0	41.0
34	35.52986424444257	38.0	34.0	40.0	27.0	41.0
35	35.44490822050473	38.0	34.0	40.0	27.0	41.0
36	35.36747319550211	38.0	34.0	40.0	27.0	41.0
37	35.21393498457871	38.0	34.0	40.0	26.0	41.0
38	35.100044896351356	38.0	33.0	40.0	26.0	41.0
39	35.072779393778845	38.0	33.0	40.0	25.0	41.0
40	34.94124383535252	38.0	33.0	40.0	25.0	41.0
41	34.784333237577385	38.0	33.0	40.0	24.0	41.0
42	34.58863245025708	38.0	33.0	40.0	24.0	41.0
43	34.258631366552045	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	5.0
15	5.0
16	18.0
17	73.0
18	191.0
19	377.0
20	907.0
21	1792.0
22	3257.0
23	5825.0
24	9657.0
25	15003.0
26	22735.0
27	33167.0
28	46397.0
29	61826.0
30	79686.0
31	98721.0
32	120102.0
33	146461.0
34	176936.0
35	210382.0
36	256513.0
37	320160.0
38	375336.0
39	339823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.70037276012791	24.77255095563862	13.115024108136561	22.412052176096907
2	18.245507354572805	22.842824926591884	35.63910214177958	23.27256557705573
3	20.002356628404424	26.60401246088771	31.59890356573359	21.794727344974273
4	13.208601177626136	15.873440453848787	35.2697823473051	35.64817602121998
5	11.64286242622635	40.059715587634756	34.72345739749096	13.573964588647932
6	32.1773526290168	35.723562327660794	15.355068213211224	16.744016830111175
7	25.102865969769788	34.57990948482727	21.456886601449412	18.860337943953528
8	27.841070356538957	33.4262796750261	20.088493976836236	18.64415599159871
9	25.331304109994342	13.604454543734379	23.223110783897173	37.841130562374104
10	16.467199000927167	27.477943162251282	33.866255317465374	22.188602519356177
11	31.910640779304327	21.447038646985668	24.88105047141169	21.76127010229831
12	20.40225238630128	26.89231240291809	31.61911552467665	21.086319686103977
13	31.19668558276668	20.476133546863363	28.51197838094468	19.815202489425275
14	21.20028073120847	22.209803574162407	27.92462745489293	28.6652882397362
15	23.455333290902555	28.54595167363621	26.37849860408471	21.62021643137653
16	22.319077164958827	28.039276566684844	26.78772626642974	22.853920001926586
17	20.021063441468748	26.776717199430973	27.60497747441682	25.597241884683463
18	21.096210644735688	26.130708588276374	31.596624344831504	21.176456422156438
19	20.321490558864966	24.508892401851586	32.82396329852289	22.345653740760554
20	24.567937124466102	23.67796586845197	31.618642478829045	20.13545452825288
21	23.908726233746574	24.531125556688956	30.465571723211415	21.094576486353056
22	22.40972995102685	24.121166823488533	31.42095231869873	22.048150906785885
23	21.61849626465797	26.013049184726984	32.26774739007705	20.100707160538
24	20.282528782689617	25.099210615492858	30.78836100794889	23.829899593868635
25	21.391176232800483	26.049430710824495	31.820633055755764	20.738760000619262
26	22.715833618594313	25.12105673281855	30.57346918063299	21.589640467954155
27	21.41547358770012	26.52970125864599	30.229865878600954	21.824959275052937
28	21.40713077911511	25.82899134584124	30.365415016023352	22.398462859020295
29	20.428914970438935	25.837721191937923	31.197932703637637	22.535431133985504
30	19.829651889861168	26.156726109894574	32.907950438556504	21.105671561687757
31	21.293212738178585	25.5120076237789	31.852241119209275	21.342538518833244
32	22.213114895095632	25.788481419619192	29.824551595540637	22.17385208974454
33	23.10824665126544	25.74586428916691	29.426849050209945	21.719040009357705
34	21.504277194545697	28.33884360072178	29.553754349871593	20.603124854860933
35	20.200347817710494	29.419753362495893	29.681648745396405	20.698250074397208
36	21.19877558532973	25.943855478472972	31.736043857370653	21.12132507882664
37	21.28499894209747	26.276363705170304	30.79236039556954	21.646276957162687
38	21.493440144218777	25.880209309886315	31.338728349551637	21.28762219634327
39	22.379713041788012	25.388929695066047	31.254354172006348	20.977003091139593
40	20.709990212251373	26.24359452918177	30.335656131792295	22.710759126774565
41	21.80552139113323	25.74724042254175	30.237305599658722	22.2099325866663
42	19.41943513165296	27.49798310452249	30.54543046312049	22.537151300704064
43	20.094557564519153	27.25006407621027	30.191893198288778	22.463485160981804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	280.5
2	357.0
3	916.0
4	1475.0
5	1475.0
6	2496.0
7	3517.0
8	3850.0
9	4183.0
10	6425.5
11	8668.0
12	8668.0
13	16090.0
14	23512.0
15	38582.0
16	53652.0
17	51822.0
18	49992.0
19	49992.0
20	54602.5
21	59213.0
22	48553.0
23	37893.0
24	41392.5
25	44892.0
26	44892.0
27	49411.0
28	53930.0
29	57382.5
30	60835.0
31	65404.0
32	69973.0
33	69973.0
34	76040.0
35	82107.0
36	90127.0
37	98147.0
38	102530.0
39	106913.0
40	106913.0
41	112921.0
42	118929.0
43	135237.5
44	151546.0
45	187115.0
46	222684.0
47	222684.0
48	228253.5
49	233823.0
50	215614.5
51	197406.0
52	186217.0
53	175028.0
54	175028.0
55	152994.5
56	130961.0
57	109739.5
58	88518.0
59	78367.0
60	68216.0
61	68216.0
62	60883.0
63	53550.0
64	46999.5
65	40449.0
66	35000.0
67	29551.0
68	29551.0
69	25335.5
70	21120.0
71	17679.5
72	14239.0
73	11416.5
74	8594.0
75	8594.0
76	6785.5
77	4977.0
78	3859.5
79	2742.0
80	2207.0
81	1672.0
82	1672.0
83	1269.0
84	866.0
85	682.0
86	498.0
87	392.0
88	286.0
89	286.0
90	233.0
91	180.0
92	109.0
93	38.0
94	26.5
95	15.0
96	15.0
97	9.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2325356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.10152849937163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22757690781295	33.92878703846397
2	13.451035138179556	12.13324489261309
3	4.750085720491617	6.427083794916327
4	2.1481022909812553	3.8753078678502626
5	1.1798131067689568	2.660568722943614
6	0.6810067858104891	1.8428668175098348
7	0.4538741557630032	1.432929271989133
8	0.30804978307602787	1.1114812856502974
9	0.21943183376848732	0.890703999394093
>10	1.3578344903653585	11.637094646992722
>50	0.13407571159098966	4.17355781177373
>100	0.07655223837219906	6.439031362725814
>500	0.005604511900838301	1.791805206227057
>1k	0.005604511900838298	4.719864373069762
>5k	9.662951553169479E-4	3.114500346787234
>10k+	3.865180621267792E-4	3.8211725610931087
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26930	1.1581022432694177	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24059	1.0346372770448913	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21376	0.919257094397589	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15314	0.6585658282000691	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9105	0.3915529493118473	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9081	0.3905208492807123	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8666	0.37267411957566926	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8421	0.36213809842449934	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7824	0.3364646101500157	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6003	0.2581540202876463	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5999	0.25798200361579043	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5799	0.24938117002299862	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5490	0.23609288212213525	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5076	0.21828915658505624	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4772	0.20521588952401265	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4662	0.20048543104797717	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3543	0.15236376709630697	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3236	0.13916148753137153	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3216	0.13830140417209236	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3173	0.1364522249496421	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3099	0.13326991652030915	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3057	0.13146374146582288	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2983	0.1282814330364899	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2667	0.11469211595987884	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2396	0.10303798644164593	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2378	0.10226391141829466	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2357	0.10136082389105151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.7201667185583628E-4	0.0
11	0.0	0.0	0.0	2.1502083981979534E-4	0.0
12	0.0	0.0	0.0	3.010291757477135E-4	0.0
13	0.0	0.0	0.0	4.300416796395907E-4	0.0
14	4.300416796395907E-5	0.0	0.0	5.590541835314678E-4	0.0
15	4.300416796395907E-5	0.0	0.0	7.740750233512632E-4	0.0
16	4.300416796395907E-5	0.0	0.0	0.0015911542146664855	0.0
17	4.300416796395907E-5	0.0	0.0	0.002623254245801503	0.0
18	4.300416796395907E-5	0.0	0.0	0.0032683167652608892	0.0
19	4.300416796395907E-5	0.0	0.0	0.003870375116756316	0.0
20	4.300416796395907E-5	0.0	0.0	0.005203504323639047	0.0
21	4.300416796395907E-5	0.0	0.0	0.007181696049981165	0.0
22	4.300416796395907E-5	0.0	0.0	0.01156812118230499	0.0
23	4.300416796395907E-5	0.0	0.0	0.018792821400250112	0.0
24	4.300416796395907E-5	0.0	0.0	0.03083398843015865	0.0
25	4.300416796395907E-5	0.0	0.0	0.03504839689062664	0.0
26	4.300416796395907E-5	0.0	0.0	0.041197992909472785	0.0
27	4.300416796395907E-5	0.0	0.0	0.08476121505696332	0.0
28	4.300416796395907E-5	0.0	0.0	0.13726930414095734	0.0
29	4.300416796395907E-5	0.0	0.0	0.19859324765756298	0.0
30	4.300416796395907E-5	0.0	0.0	0.3103610801958926	0.0
31	4.300416796395907E-5	0.0	0.0	0.46483205152243356	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6245	0.0	28.97198	1
GTATTGG	805	0.0	20.223602	1
ATTGGAC	875	0.0	19.028572	3
TTGGACC	1410	0.0	18.5	4
GTATCAA	10545	0.0	17.842106	2
TGGACCC	1425	0.0	17.785965	5
TCTAACG	85	2.7250571E-5	17.411764	2
GGACCCT	1485	0.0	17.19192	6
AAGACCG	165	5.4569682E-11	16.818182	5
AATGCGT	90	4.4491062E-5	16.444445	35
CGATCGA	70	0.0025935639	15.857142	1
ACAGCGT	130	2.5920963E-7	15.653846	8
AGTATCA	450	0.0	15.622222	1
TATTGGA	1025	0.0	15.521951	2
CGTCTGT	525	0.0	15.504762	34
GACCCTC	1690	0.0	14.997041	7
ATTAGAC	210	9.094947E-12	14.97619	3
TATACCG	150	8.111965E-8	14.8	5
TATACTG	250	0.0	14.799999	5
GACCGTG	180	3.3360266E-9	14.388888	7
>>END_MODULE