FastQCFastQC Report
Wed 25 May 2016
SRR2088238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719955
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT382471.406162969607953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT339321.247520639128221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT306911.1283642560262945No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG218780.8043515425806678No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129340.47552257298374423No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA128760.4733901847640862No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT126460.46493416251371805No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA121470.44658827076183244No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT116980.4300806447165486No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA91770.3373952877896877No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT87270.3208508964302718No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG86160.31676994656161594No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT81670.30026232051633206No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA70650.2597469443428292No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA69840.2567689538981343No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA67950.24982030952717965No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT54690.2010695029881009No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA48210.17724557943054206No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45750.16820131215406137No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG45690.16798072026926916No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC45590.1676130671279488No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG44290.1628335762907842No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC44150.16231886189293573No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39340.14463474579542676No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37670.13849493833537688No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC35380.1300756813991408No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA32110.11805342367796526No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC31470.11570044357351501No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG31120.11441365757889375No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.1133106981549327No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA30470.11202391216031148No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA30290.11136213650593484No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28770.10577380875786548No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG28770.10577380875786548No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC27720.10191345077400177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84450.030.0337491
GTATTGG10450.024.7846891
ATTGGAC11000.023.7136363
TATTGGA12500.021.3119982
TTGGACC18650.019.8391424
ACGTTTA951.6782178E-719.47368426
ACGCATA902.1544165E-618.57
GCGCATA1205.182301E-918.526
CGCCGGA1950.018.02564214
GGACCCT20150.017.7196046
CGTCTGT7750.017.66451634
GTATCAA150450.017.63312
TGGACCC21200.017.6273575
GCCGGAA2100.017.61904715
AGTATCA6250.016.8719981
ACGGACC1005.883734E-616.658
TCGTGTC2800.016.51785934
TCTAACG904.4495508E-516.4444452
TATACCG2500.016.2799995
CGCTATC700.00259371515.85714315
Produced by FastQC (version 0.11.5)