FastQCFastQC Report
Wed 25 May 2016
SRR2088237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109364
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148211.3359907117952268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125251.1290252793492488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96180.8669832444535788No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG58100.5237235028358591No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46510.41924922748529786No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT40360.3638120580801252No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT38720.3490288129054125No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38130.3437104503120707No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA35770.3224369999387036No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA28120.253478569703001No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG27630.24906162449836122No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT23480.21165280286722846No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT23140.20858798374564164No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA22340.2013766446360257No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA18470.16649179169325848No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.15233953869063716No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA16000.1442267821923192No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA15560.14026054568203042No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT14710.13259849787806346No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14110.1271899935458515No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC13830.12466602485748592No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG12280.110694055332605No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC12220.1101532048993838No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.10979263794390298No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC12040.1085306535997202No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT11850.1068179605611864No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11290.10177002318445523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30800.030.5129851
ACGGACC400.001931144923.1250028
CGTGCGG450.003825725520.55555510
TAAACGT656.901853E-519.9230774
GTATCAA49950.019.259262
GTATTGG3300.019.0606061
GTAGACC609.2365383E-418.54
ATTGGAC3550.017.718313
TATACTG1900.017.5263165
CGTCTGT2650.017.45283134
GGACCCT5450.016.6330286
TTGGACC5900.016.3050844
GTGGTAT4900.015.8571421
TATTGGA4100.015.3414642
TACCGTG855.3639436E-415.2352937
GTAGTGT1101.451698E-515.1363646
TAGACAG1601.0959411E-815.0312515
TGGACCC6050.014.9834725
AACGTGA750.00410428714.86
CTTGCGC750.00410428714.88
Produced by FastQC (version 0.11.5)