##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109364
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.73207080813872	31.0	30.0	31.0	26.0	34.0
2	30.501955174316095	31.0	30.0	33.0	27.0	34.0
3	30.479643291110943	31.0	30.0	34.0	27.0	34.0
4	34.63255432842602	35.0	35.0	37.0	32.0	37.0
5	34.48573506982379	35.0	35.0	37.0	30.0	37.0
6	34.638778615495006	35.0	35.0	37.0	31.0	37.0
7	34.24610227121125	35.0	35.0	37.0	30.0	37.0
8	34.4916303395459	35.0	35.0	37.0	30.0	37.0
9	35.86872838851811	37.0	35.0	39.0	30.0	39.0
10	35.73041670723045	37.0	35.0	39.0	30.0	39.0
11	35.865361594571304	37.0	35.0	39.0	30.0	39.0
12	35.56929105325214	37.0	35.0	39.0	30.0	39.0
13	35.763683515960494	37.0	35.0	39.0	30.0	39.0
14	36.58764120703394	38.0	35.0	40.0	30.0	41.0
15	36.65379172210383	38.0	35.0	40.0	31.0	41.0
16	36.54318240000577	38.0	35.0	40.0	30.0	41.0
17	36.63914549237221	38.0	35.0	40.0	30.0	41.0
18	36.629890640042404	38.0	35.0	40.0	30.0	41.0
19	36.71728576012923	38.0	35.0	40.0	30.0	41.0
20	36.67766125455667	38.0	35.0	40.0	30.0	41.0
21	36.61320540417753	38.0	35.0	40.0	30.0	41.0
22	36.52784838880656	38.0	35.0	40.0	30.0	41.0
23	36.391570305147816	38.0	35.0	40.0	30.0	41.0
24	36.32178978225362	38.0	35.0	40.0	30.0	41.0
25	36.21907687648058	38.0	34.0	40.0	30.0	41.0
26	35.95091241468084	38.0	34.0	40.0	29.0	41.0
27	35.774912472371554	38.0	34.0	40.0	28.0	41.0
28	35.5997715808337	38.0	34.0	40.0	27.0	41.0
29	35.417532928777206	38.0	34.0	40.0	27.0	41.0
30	35.240747851922364	38.0	34.0	40.0	27.0	41.0
31	34.989782433899066	37.0	33.0	40.0	26.0	41.0
32	34.758311068323835	37.0	33.0	40.0	25.0	41.0
33	34.92440353211389	37.0	33.0	40.0	26.0	41.0
34	34.908334865742894	37.0	33.0	40.0	26.0	41.0
35	34.818322029559276	37.0	33.0	40.0	25.0	41.0
36	34.73188691899142	37.0	33.0	40.0	25.0	41.0
37	34.58433480805218	37.0	33.0	40.0	25.0	41.0
38	34.45603516970084	37.0	33.0	40.0	24.0	41.0
39	34.426850880324224	37.0	33.0	40.0	24.0	41.0
40	34.28902235875691	37.0	33.0	40.0	24.0	41.0
41	34.121561543370795	37.0	33.0	40.0	23.0	41.0
42	33.92885202692714	37.0	33.0	40.0	22.0	41.0
43	33.56255115543681	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	11.0
16	33.0
17	73.0
18	147.0
19	345.0
20	676.0
21	1311.0
22	2303.0
23	3923.0
24	6387.0
25	9624.0
26	14197.0
27	20070.0
28	27007.0
29	34979.0
30	44278.0
31	53817.0
32	64455.0
33	76605.0
34	90262.0
35	105530.0
36	126333.0
37	152283.0
38	171723.0
39	102991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.334577289329744	26.493558471340332	14.053547798558451	21.11831644077147
2	19.746539458644776	24.554339243025733	33.78259975986241	21.916521538467084
3	21.681341741754736	26.623813284007774	31.659220959035988	20.035624015201503
4	15.009050230582568	17.659127211627563	34.53014520031297	32.8016773574769
5	11.384090343656366	41.18585063153302	34.626957427859566	12.803101596951047
6	32.13192423767131	36.82452287977616	14.792980482510702	16.250572400041825
7	24.101737572158463	35.4027172325765	21.880645126396747	18.61490006886829
8	27.131942266019088	34.921089921793026	20.19851013733995	17.74845767484793
9	24.824494034419722	13.891563093808704	23.600098795345804	37.68384407642577
10	16.328995712858898	28.699867671927336	34.01913168265781	20.95200493255595
11	31.942085735610675	22.05939619457635	24.395058790442093	21.60345927937088
12	19.431043372599074	28.039038584269903	32.00969203976332	20.520226003367696
13	30.66567871320865	21.20386095095929	28.836071839360212	19.294388496471854
14	20.55312773805532	22.955224795468396	28.730245437926595	27.761402028549693
15	23.26576308587623	29.490500863557855	26.460927161869325	20.782808888696586
16	21.629870808859852	28.634785336463054	27.5575014152253	22.177842439451794
17	19.3653300449627	27.747610342502547	28.579077741841267	24.307981870693478
18	20.254307873700604	26.474177997483245	32.365301199606265	20.90621292920989
19	19.683530383174503	25.375260058916645	33.77701097205246	21.1641985858564
20	23.493641401740096	24.579038079476167	32.72073007597145	19.206590442812278
21	23.180308717427284	24.902015929848094	31.52968728027951	20.38798807244511
22	21.62130734366718	24.911570954168337	32.18817268272632	21.278949019438166
23	20.817693741639353	26.977799892551047	32.817001453084835	19.38750491272477
24	19.969189553654164	25.75466663782131	31.663637904240627	22.612505904283896
25	20.64381032735874	26.786248697451875	32.45553308021533	20.114407894974057
26	21.850357502136358	25.78639652990362	31.428998957961497	20.934247009998522
27	21.078383650451972	26.90568650145489	31.149829992680488	20.86609985541265
28	20.665083777732107	26.724321322848045	31.373201221600844	21.237393677819004
29	20.015432265694578	26.32454271095871	32.072430690016986	21.587594333329726
30	19.457905610782394	26.714946582005545	33.546788970977964	20.280358836234093
31	20.618841065691694	26.18031592876639	32.56830039554195	20.632542609999962
32	21.29977176111718	26.7265748663198	30.807742093668082	21.16591127889493
33	22.199115889825162	26.609751172744023	30.41751850609899	20.773614431331826
34	20.855553271964837	28.66948990592808	30.640258742847255	19.834698079259827
35	19.652251199786544	29.524123732156443	30.737521679088196	20.086103388968816
36	20.67968673942908	26.407112543763816	32.44922315849442	20.463977558312692
37	20.4140390349786	27.034679329778143	31.77658550304499	20.77469613219827
38	20.920004615257028	26.573153626762725	32.0013088580484	20.505532899931854
39	21.72515062684565	26.127763295005067	31.86420327322682	20.282882804922462
40	20.247366959807604	26.928221936172434	31.10782394236698	21.716587161652985
41	21.01564500019831	26.50374448783267	30.97234090884507	21.508269603123953
42	19.00927017642541	27.989190202674685	31.110888761488564	21.89065085941134
43	19.58230121042327	27.702719756545193	30.79313913197111	21.921839901060427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	117.5
2	145.0
3	344.5
4	544.0
5	544.0
6	1055.5
7	1567.0
8	1778.5
9	1990.0
10	3185.5
11	4381.0
12	4381.0
13	8361.5
14	12342.0
15	20264.5
16	28187.0
17	26807.0
18	25427.0
19	25427.0
20	28958.0
21	32489.0
22	26815.0
23	21141.0
24	23093.0
25	25045.0
26	25045.0
27	27718.5
28	30392.0
29	32216.0
30	34040.0
31	36927.5
32	39815.0
33	39815.0
34	43173.0
35	46531.0
36	50120.5
37	53710.0
38	55297.0
39	56884.0
40	56884.0
41	59053.5
42	61223.0
43	66627.0
44	72031.0
45	86523.5
46	101016.0
47	101016.0
48	102536.0
49	104056.0
50	95528.0
51	87000.0
52	81302.0
53	75604.0
54	75604.0
55	65826.0
56	56048.0
57	47292.5
58	38537.0
59	33823.5
60	29110.0
61	29110.0
62	25482.0
63	21854.0
64	19039.0
65	16224.0
66	13775.0
67	11326.0
68	11326.0
69	9588.5
70	7851.0
71	6585.5
72	5320.0
73	4278.5
74	3237.0
75	3237.0
76	2562.5
77	1888.0
78	1469.5
79	1051.0
80	805.0
81	559.0
82	559.0
83	446.0
84	333.0
85	262.0
86	191.0
87	145.5
88	100.0
89	100.0
90	81.0
91	62.0
92	38.0
93	14.0
94	10.0
95	6.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1109364.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.97574235720772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.50708238663617	45.624194439620496
2	11.3003419665564	12.650900609366062
3	3.2076743519657724	5.386558592743781
4	1.2914339833207094	2.8915590368681015
5	0.6659978397071925	1.863986174295337
6	0.4194270245969695	1.4086643439894115
7	0.2706126888837365	1.0603422306083072
8	0.1785307742759841	0.7994714098964236
9	0.14546753096750925	0.7328387731298792
>10	0.8938372795414959	9.508423080605954
>50	0.0683255291168432	2.609742433425032
>100	0.04056063759476618	4.455728284682977
>500	0.0056784884738814885	2.1360031243646853
>1k	0.004380548251280005	4.98700423334345
>5k	3.2448505565037077E-4	1.40111633514874
>10k+	3.2448505565037077E-4	2.4834668979114234
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14821	1.3359907117952268	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12525	1.1290252793492488	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9618	0.8669832444535788	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5810	0.5237235028358591	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4651	0.41924922748529786	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4036	0.3638120580801252	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3872	0.3490288129054125	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3813	0.3437104503120707	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3577	0.3224369999387036	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2812	0.253478569703001	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2763	0.24906162449836122	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2348	0.21165280286722846	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2314	0.20858798374564164	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2234	0.2013766446360257	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1847	0.16649179169325848	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1690	0.15233953869063716	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1600	0.1442267821923192	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1556	0.14026054568203042	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1471	0.13259849787806346	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1411	0.1271899935458515	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1383	0.12466602485748592	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1228	0.110694055332605	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1222	0.1101532048993838	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1218	0.10979263794390298	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1204	0.1085306535997202	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1185	0.1068179605611864	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1129	0.10177002318445523	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	9.01417388701995E-5	0.0	0.0	0.0	0.0
9	9.01417388701995E-5	0.0	0.0	0.0	0.0
10	9.01417388701995E-5	0.0	0.0	9.01417388701995E-5	0.0
11	9.01417388701995E-5	0.0	0.0	2.704252166105985E-4	0.0
12	9.01417388701995E-5	0.0	0.0	4.507086943509975E-4	0.0
13	9.01417388701995E-5	0.0	0.0	4.507086943509975E-4	0.0
14	9.01417388701995E-5	0.0	0.0	7.21133910961596E-4	0.0
15	9.01417388701995E-5	0.0	0.0	9.01417388701995E-4	0.0
16	9.01417388701995E-5	0.0	0.0	0.001622551299663591	0.0
17	9.01417388701995E-5	0.0	0.0	0.0024338269494953864	0.0
18	9.01417388701995E-5	0.0	0.0	0.002523968688365586	0.0
19	9.01417388701995E-5	0.0	0.0	0.003245102599327182	0.0
20	9.01417388701995E-5	0.0	0.0	0.004597228682380175	0.0
21	9.01417388701995E-5	0.0	0.0	0.005859213026562967	0.0
22	9.01417388701995E-5	0.0	0.0	0.009645166059111346	0.0
23	9.01417388701995E-5	0.0	0.0	0.016856505168727307	0.0
24	9.01417388701995E-5	0.0	0.0	0.02578053731687706	0.0
25	9.01417388701995E-5	0.0	0.0	0.030828474693608228	0.0
26	9.01417388701995E-5	0.0	0.0	0.03776938858661359	0.0
27	9.01417388701995E-5	0.0	0.0	0.07923458846690536	0.0
28	9.01417388701995E-5	0.0	0.0	0.12268290660234152	0.0
29	9.01417388701995E-5	0.0	0.0	0.17126930385337905	0.0
30	9.01417388701995E-5	0.0	0.0	0.27538301224845946	0.0
31	9.01417388701995E-5	0.0	0.0	0.40671952578234016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3080	0.0	30.512985	1
ACGGACC	40	0.0019311449	23.125002	8
CGTGCGG	45	0.0038257255	20.555555	10
TAAACGT	65	6.901853E-5	19.923077	4
GTATCAA	4995	0.0	19.25926	2
GTATTGG	330	0.0	19.060606	1
GTAGACC	60	9.2365383E-4	18.5	4
ATTGGAC	355	0.0	17.71831	3
TATACTG	190	0.0	17.526316	5
CGTCTGT	265	0.0	17.452831	34
GGACCCT	545	0.0	16.633028	6
TTGGACC	590	0.0	16.305084	4
GTGGTAT	490	0.0	15.857142	1
TATTGGA	410	0.0	15.341464	2
TACCGTG	85	5.3639436E-4	15.235293	7
GTAGTGT	110	1.451698E-5	15.136364	6
TAGACAG	160	1.0959411E-8	15.031251	5
TGGACCC	605	0.0	14.983472	5
AACGTGA	75	0.004104287	14.8	6
CTTGCGC	75	0.004104287	14.8	8
>>END_MODULE