FastQCFastQC Report
Wed 25 May 2016
SRR2088236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3284491
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT381241.1607277961790732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT339421.0334021314109247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT308850.9403283492023573No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG216070.657849267968766No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135990.41403675638021237No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA132380.4030457078433158No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA128990.3927244738986954No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT120170.3658709979719841No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT113230.3447413921974516No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA86070.26204973616916594No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG83520.2542859761223276No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT82470.2510891337501001No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT80580.24533481748009053No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA71790.21857267990687143No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA70200.21373174717178398No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA66140.20137062333250416No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT51570.15701062965311824No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC47120.14346210721843963No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA46870.14270095427267118No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44990.13697708412049234No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG44810.13642905399953903No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC44480.13542433211112467No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG43110.1312532139683135No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT40590.12358079227496742No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC35500.10808371829912153No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34050.10366903121366447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92200.028.5726681
GTATTGG11150.022.0672651
ATTGGAC12500.019.6843
AACACCG701.2200727E-418.55
GTATCAA155300.017.3802322
TATTGGA15250.017.2262292
TTGGACC21250.017.1505894
AGCCGTC2350.016.53191612
TGGACCC22100.015.8212665
GGACCCT22250.015.7977526
CGAATTA2250.015.62222215
CGTCTGT9850.015.21319934
CCGTCGT1101.4534227E-515.1363649
GACCCTC23400.014.9423077
ACAACGC3600.014.9027783
TGCGACG1752.242814E-914.79999922
GCCGTCA2750.014.79999913
TCGACGG908.2797854E-414.38888819
TACACAG4850.014.1134025
CGACTAT1051.6571094E-414.09523836
Produced by FastQC (version 0.11.5)