##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088236_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3284491
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.902004602844094	31.0	30.0	33.0	27.0	34.0
2	30.78346294753129	31.0	31.0	34.0	27.0	34.0
3	30.685726037915767	31.0	31.0	34.0	27.0	34.0
4	34.93383206104081	35.0	35.0	37.0	32.0	37.0
5	34.87962914192793	35.0	35.0	37.0	32.0	37.0
6	34.997982640232536	36.0	35.0	37.0	32.0	37.0
7	34.56366937829941	35.0	35.0	37.0	30.0	37.0
8	34.83495829338549	35.0	35.0	37.0	32.0	37.0
9	36.2678752963549	38.0	35.0	39.0	32.0	39.0
10	36.206475523909184	38.0	35.0	39.0	32.0	39.0
11	36.29965160507366	38.0	35.0	39.0	32.0	39.0
12	36.03267934057362	38.0	35.0	39.0	30.0	39.0
13	36.209101501572086	38.0	35.0	39.0	32.0	39.0
14	37.12827345241622	39.0	36.0	40.0	31.0	41.0
15	37.17549994808937	39.0	36.0	40.0	32.0	41.0
16	37.09487040762176	39.0	36.0	40.0	31.0	41.0
17	37.187111792968835	39.0	36.0	40.0	31.0	41.0
18	37.18097172438591	39.0	36.0	40.0	31.0	41.0
19	37.26275608610284	39.0	36.0	40.0	31.0	41.0
20	37.217398982064495	39.0	36.0	40.0	31.0	41.0
21	37.1491342189703	39.0	36.0	40.0	31.0	41.0
22	37.0751656801617	39.0	36.0	40.0	31.0	41.0
23	36.957791633467714	39.0	36.0	40.0	31.0	41.0
24	36.88174940957366	39.0	35.0	40.0	31.0	41.0
25	36.80077126105689	39.0	35.0	40.0	30.0	41.0
26	36.53517455216044	38.0	35.0	40.0	30.0	41.0
27	36.40464352010707	38.0	35.0	40.0	30.0	41.0
28	36.24931656077	38.0	35.0	40.0	30.0	41.0
29	36.088592113663886	38.0	35.0	40.0	30.0	41.0
30	35.931766900868354	38.0	34.0	40.0	29.0	41.0
31	35.685807024589195	38.0	34.0	40.0	29.0	41.0
32	35.439016882676796	38.0	34.0	40.0	27.0	41.0
33	35.59433988401856	38.0	34.0	40.0	28.0	41.0
34	35.58868147301972	38.0	34.0	40.0	28.0	41.0
35	35.507921318706614	38.0	34.0	40.0	27.0	41.0
36	35.435076241645966	38.0	34.0	40.0	27.0	41.0
37	35.279239005374045	38.0	34.0	40.0	26.0	41.0
38	35.16200409743854	38.0	34.0	40.0	26.0	41.0
39	35.1357839007627	38.0	34.0	40.0	25.0	41.0
40	34.99483846964416	38.0	33.0	40.0	25.0	41.0
41	34.840353041003915	38.0	33.0	40.0	24.0	41.0
42	34.6468408651447	38.0	33.0	40.0	24.0	41.0
43	34.317303959730744	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	11.0
16	33.0
17	114.0
18	235.0
19	640.0
20	1227.0
21	2483.0
22	4507.0
23	7992.0
24	13006.0
25	20875.0
26	31420.0
27	45703.0
28	64424.0
29	86169.0
30	110710.0
31	138620.0
32	169109.0
33	205729.0
34	248042.0
35	296917.0
36	361286.0
37	451850.0
38	528166.0
39	495219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.33562308436833	25.199764590616937	13.297281070339363	22.167331254675382
2	17.962022121540294	22.900291095332577	35.99927660024034	23.138410182886783
3	19.814363930362422	26.830763122809593	31.787086644475504	21.56778630235248
4	13.097402306780564	15.854389614707424	35.52580902185453	35.522399056657484
5	11.371777240369969	40.30633665916576	34.937133333597195	13.384752766867072
6	31.99710396527194	36.03748647811792	15.300209377952322	16.66520017865782
7	24.728184671536624	34.93716377971503	21.644327842578956	18.690323706169387
8	27.532576584925945	33.75284024221713	20.20848892568133	18.50609424717559
9	25.121244052731456	13.699261164058601	23.51502866045302	37.66446612275692
10	16.227476342605293	27.699725771816695	34.01321544190561	22.059582443672397
11	31.622586269836027	21.671120426270008	25.026313057335216	21.679980246558753
12	20.127502252251567	27.08885486366076	31.8395757516157	20.94406713247197
13	30.959317592893388	20.72540311421161	28.73608117665721	19.579198116237798
14	20.97868436844552	22.454773053115385	28.144056415438495	28.4224861630006
15	23.085677506803943	28.917448700574916	26.61788386693707	21.37898992568407
16	22.09222068198695	28.374533527417185	26.971180618244954	22.562065172350906
17	19.67872647542648	27.00875112764809	27.823154333502515	25.48936806342292
18	20.84176817656069	26.294272080514148	31.998139133278187	20.865820609646974
19	19.963915261147008	24.84019593903591	33.25114911260222	21.944739687214852
20	24.32571743993209	23.868568980703557	31.975669898319097	19.83004368104525
21	23.729643345041897	24.698621491122978	30.77785873062219	20.79387643321294
22	22.029714802080445	24.39848366154756	31.679794525239984	21.892007011132016
23	21.14711229228517	26.366946963776122	32.693284895589606	19.792655848349106
24	19.993082642028856	25.39011371929471	31.016160494883376	23.600643143793057
25	21.142697605199707	26.267388158469608	32.1579203596539	20.43199387667678
26	22.487685306490413	25.328186315626986	30.860215479354338	21.323912898528267
27	21.10272185248795	26.783906547468085	30.39868277915817	21.714688820885794
28	20.977557862085785	26.077252152616648	30.704788047828412	22.240401937469155
29	20.06965462837316	26.091866289175403	31.542025842055892	22.296453240395543
30	19.512764687131128	26.380343255621646	33.278702849239046	20.828189208008183
31	20.87233607886275	25.794407717969086	32.28110535239706	21.052150850771092
32	21.836016600441287	25.98795368901909	30.20988640248976	21.966143308049862
33	22.74041853060337	25.921794274972896	29.861339245563467	21.476447948860265
34	21.11173390336585	28.586377615283464	29.84017919367111	20.461709287679582
35	19.85016856493137	29.728015695582666	30.020237534522092	20.401578204963876
36	20.910363280033344	26.108124516097014	32.01369709948969	20.967815104379948
37	20.854098854282142	26.454083753007694	31.21214215535984	21.479675237350325
38	21.07921744952262	26.060324111102755	31.744583863983795	21.11587457539083
39	22.032972536688334	25.534915455697703	31.674253331794787	20.757858675819175
40	20.3899173418347	26.421019269043516	30.742175880524563	22.44688750859722
41	21.458271616515315	25.780615626591764	30.694893059533424	22.0662196973595
42	19.039784246630603	27.611827829639356	31.034732626760132	22.313655296969912
43	19.687525403479565	27.36162163330635	30.63908532554968	22.311767637664406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	251.0
1	359.5
2	468.0
3	1202.0
4	1936.0
5	1936.0
6	3540.5
7	5145.0
8	5709.0
9	6273.0
10	9700.0
11	13127.0
12	13127.0
13	24111.0
14	35095.0
15	56156.0
16	77217.0
17	75482.0
18	73747.0
19	73747.0
20	79661.5
21	85576.0
22	70237.0
23	54898.0
24	60510.0
25	66122.0
26	66122.0
27	73201.5
28	80281.0
29	84782.0
30	89283.0
31	96729.0
32	104175.0
33	104175.0
34	113950.0
35	123725.0
36	133804.0
37	143883.0
38	150073.5
39	156264.0
40	156264.0
41	163632.5
42	171001.0
43	194122.5
44	217244.0
45	268138.0
46	319032.0
47	319032.0
48	324739.0
49	330446.0
50	304987.0
51	279528.0
52	262220.0
53	244912.0
54	244912.0
55	211532.0
56	178152.0
57	147539.0
58	116926.0
59	102892.5
60	88859.0
61	88859.0
62	78249.5
63	67640.0
64	59157.0
65	50674.0
66	43045.5
67	35417.0
68	35417.0
69	30186.5
70	24956.0
71	21027.0
72	17098.0
73	13909.0
74	10720.0
75	10720.0
76	8403.5
77	6087.0
78	4862.0
79	3637.0
80	2927.5
81	2218.0
82	2218.0
83	1664.0
84	1110.0
85	873.5
86	637.0
87	522.5
88	408.0
89	408.0
90	321.5
91	235.0
92	148.5
93	62.0
94	40.0
95	18.0
96	18.0
97	11.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3284491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.77709763698045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86216346313563	28.18691194840815
2	14.634704710476761	11.642521563140239
3	5.875399369311709	7.0111900310809565
4	2.7588027087977687	4.3894865883606
5	1.5988608109976008	3.1799021293496663
6	0.9756007992944173	2.328394094895012
7	0.6099702262742307	1.6983991672312797
8	0.43917026779413565	1.397513489704495
9	0.3038008101928382	1.0875883040310863
>10	1.6607168765179723	12.424708695591931
>50	0.1668280296233672	4.657163941284289
>100	0.10019159286243073	7.424607462905078
>500	0.006197903245511421	1.7120287305181496
>1k	0.006275377036016038	5.251895821057475
>5k	6.197903245447939E-4	1.8254038308474707
>10k+	6.972641151128931E-4	5.782284201594258
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38124	1.1607277961790732	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33942	1.0334021314109247	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30885	0.9403283492023573	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	21607	0.657849267968766	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13599	0.41403675638021237	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13238	0.4030457078433158	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12899	0.3927244738986954	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	12017	0.3658709979719841	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11323	0.3447413921974516	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8607	0.26204973616916594	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8352	0.2542859761223276	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8247	0.2510891337501001	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8058	0.24533481748009053	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7179	0.21857267990687143	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7020	0.21373174717178398	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6614	0.20137062333250416	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5157	0.15701062965311824	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4712	0.14346210721843963	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4687	0.14270095427267118	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4499	0.13697708412049234	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4481	0.13642905399953903	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4448	0.13542433211112467	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4311	0.1312532139683135	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4059	0.12358079227496742	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3550	0.10808371829912153	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3405	0.10366903121366447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.0446117830738462E-5	0.0	0.0	0.0	0.0
3	3.0446117830738462E-5	0.0	0.0	0.0	0.0
4	3.0446117830738462E-5	0.0	0.0	0.0	0.0
5	3.0446117830738462E-5	0.0	0.0	0.0	0.0
6	3.0446117830738462E-5	0.0	0.0	0.0	0.0
7	3.0446117830738462E-5	0.0	0.0	0.0	0.0
8	3.0446117830738462E-5	0.0	0.0	0.0	0.0
9	3.0446117830738462E-5	0.0	0.0	0.0	0.0
10	3.0446117830738462E-5	0.0	0.0	9.133835349221538E-5	0.0
11	3.0446117830738462E-5	0.0	0.0	1.522305891536923E-4	0.0
12	3.0446117830738462E-5	0.0	0.0	2.1312282481516923E-4	0.0
13	3.0446117830738462E-5	0.0	0.0	2.7401506047664616E-4	0.0
14	3.0446117830738462E-5	0.0	0.0	4.2624564963033846E-4	0.0
15	3.0446117830738462E-5	0.0	0.0	0.0010351680062451077	0.0
16	6.0892235661476924E-5	0.0	0.0	0.001978997658998	0.0
17	9.133835349221538E-5	0.0	0.0	0.0031359501365660616	0.0
18	9.133835349221538E-5	0.0	0.0	0.0034708574327041845	0.0
19	9.133835349221538E-5	0.0	0.0	0.004171118142811169	0.0
20	9.133835349221538E-5	0.0	0.0	0.005449855091702185	0.0
21	9.133835349221538E-5	0.0	0.0	0.007824652282499785	0.0
22	9.133835349221538E-5	0.0	0.0	0.01245246219277203	0.0
23	9.133835349221538E-5	0.0	0.0	0.020855590714055846	0.0
24	9.133835349221538E-5	0.0	0.0	0.0332471606711664	0.0
25	9.133835349221538E-5	0.0	0.0	0.038849246352022276	0.0
26	9.133835349221538E-5	0.0	0.0	0.04618676074923025	0.0
27	9.133835349221538E-5	0.0	0.0	0.09770159211883972	0.0
28	9.133835349221538E-5	0.0	0.0	0.15280906539247635	0.0
29	9.133835349221538E-5	0.0	0.0	0.2173852813114726	0.0
30	9.133835349221538E-5	0.0	0.0	0.3394437676949031	0.0
31	9.133835349221538E-5	0.0	0.0	0.5119514713238672	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9220	0.0	28.572668	1
GTATTGG	1115	0.0	22.067265	1
ATTGGAC	1250	0.0	19.684	3
AACACCG	70	1.2200727E-4	18.5	5
GTATCAA	15530	0.0	17.380232	2
TATTGGA	1525	0.0	17.226229	2
TTGGACC	2125	0.0	17.150589	4
AGCCGTC	235	0.0	16.531916	12
TGGACCC	2210	0.0	15.821266	5
GGACCCT	2225	0.0	15.797752	6
CGAATTA	225	0.0	15.622222	15
CGTCTGT	985	0.0	15.213199	34
CCGTCGT	110	1.4534227E-5	15.136364	9
GACCCTC	2340	0.0	14.942307	7
ACAACGC	360	0.0	14.902778	3
TGCGACG	175	2.242814E-9	14.799999	22
GCCGTCA	275	0.0	14.799999	13
TCGACGG	90	8.2797854E-4	14.388888	19
TACACAG	485	0.0	14.113402	5
CGACTAT	105	1.6571094E-4	14.095238	36
>>END_MODULE