FastQCFastQC Report
Wed 25 May 2016
SRR2088235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608593
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT385171.4765431019710626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT353201.3539866127065434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302131.1582105755861494No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG172300.6605093243752475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133760.5127668440419797No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT115810.4439558029941812No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA106780.40933944084033047No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT101460.38894530499775165No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA93420.3581240921830274No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA80530.30871048109076427No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT75070.2877796574628545No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG73820.2829878022366847No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT72880.279384327106605No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60630.23242414589014077No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA59980.22993238117253248No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA56060.21490512318326394No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT50890.19508600996782557No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48700.18669067961157607No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA44170.16932499627193665No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC41880.16054631749759354No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG39840.15272600976848438No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37250.14279728573986053No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG37190.14256727668900437No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.14222226311272015No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34000.13033846215181902No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC33110.12692666123078608No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA30120.11546454352978791No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC29600.11347113175570127No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29540.1132411227048451No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC28510.10929263399848117No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC27200.10427076972145521No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG26860.10296738509993703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83100.029.6979541
CGTCTGT9400.021.64893734
ATTGGAC8100.020.5555553
ATCACGT2000.020.34999823
CGTATTG759.273175E-619.7333325
ACGTTTA2000.019.42526
TCGTATT902.1543474E-618.54
GTATTGG8450.018.1715981
TCACGTT2150.018.06976724
GTATCAA144700.017.771252
TTGGACC14650.017.300344
CCGCTAG752.0685353E-417.26666614
TATTGGA9850.017.0913722
AGTATCA5850.017.0769231
CGATCTG3050.016.98360633
GGACCCT14300.016.9475526
TATTGCG1107.816943E-716.8181827
TGCGTCT12150.016.29218132
TGGACCC15550.016.0610925
GACCCTC14450.016.003467
Produced by FastQC (version 0.11.5)