FastQCFastQC Report
Wed 25 May 2016
SRR2088234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738371
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT139061.8833350713936488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT124881.691290692619293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94291.2770003155595222No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG50850.6886781848149508No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43920.5948229277693734No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT38900.5268354255516536No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA35460.48024638020724No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT34410.4660258867154859No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30210.4091439127484693No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA28290.3831407246492617No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG24880.3369579791188982No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT23690.32084141982824355No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT22250.30133902875383783No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA21180.2868476687193836No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA16870.22847592876751663No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.2253609635264657No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT16610.22495466371241557No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA15630.2116822031201117No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14120.19123177914625575No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC13840.18743964754845466No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.17755301873990176No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG12920.174979786584251No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG12210.16536402431839822No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11940.16170732599194715No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT11180.15141439736934414No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA11050.1496537648417936No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC9610.13015137376738795No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.12920334086793767No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC9450.1279844414257873No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC9330.12635924216958683No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC9240.1251403427274365No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC9060.12270254384313577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC8970.12148364440098543No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA8680.11755607953183426No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG8580.11620174681833387No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8280.11213874867783268No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA7890.10685685109518114No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA7760.10509621856763063No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC7750.1049607852962806No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.10387731912548027No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG7630.10333558604008013No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA7560.10238755314062986No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA7520.10184582005522969No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA7520.10184582005522969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.029.8629421
TCCGACC250.005495087729.5999988
AGTTCCG250.005495087729.5999985
ATTGGAC1400.027.753
GTACACT358.8663795E-426.428574
GTATTGG1600.023.1251
CGTCTGT2150.022.37209334
TATTGGA1700.021.7647062
TATACCG450.003824531320.5555575
AGTATCA1550.020.2903231
TTGGACC3250.019.3538464
GTATCAA49600.018.6118972
CAAGACG801.615395E-518.54
GGACAGA1103.8460712E-818.56
AGCCGCA500.007032559718.49999817
TAGACTG500.007032559718.4999985
GTTCCGA500.007032559718.4999986
CGTGTCT1156.3957486E-817.69565235
TAGTCCT1054.7915273E-717.6190474
TTTTTAC6400.017.343751
Produced by FastQC (version 0.11.5)