FastQCFastQC Report
Wed 25 May 2016
SRR2088233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607802
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91221.50081770050115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86321.4201993412328358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75801.2471166597016792No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG47610.7833143030131523No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33100.544585243220654No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA28930.4759773742106804No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT28660.4715351380877325No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT26600.4376425217422779No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA23460.3859809609050316No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA20490.3371163635526043No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT19460.32017005537987697No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT19210.3160568737845548No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG19010.3127663285082971No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA16880.2777220213161523No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16130.2653824765301858No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA15120.24876522288508426No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT14730.24234865959638172No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.19002898970388382No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA11040.18163809924942662No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC10760.1770313358626658No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9730.16008502768993849No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG9670.15909786410706117No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG9660.15893333684324829No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.1467583193210947No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT8770.14429041036390142No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC8580.14116439235145656No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG8110.1334316109522509No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA7750.12750862945498698No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.12487619323398079No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC7100.11681435730714937No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC7070.11632077551571071No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC7030.11566266646045917No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC6600.10858799411650505No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA6460.10628461242312463No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA6270.1031585944106798No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA6230.10250048535542826No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC6200.1020069035639896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG200.001841253737.023
GACGTCG251.2329414E-436.99999636
ACGTCGC251.2329414E-436.99999637
TGCGGCG303.5975012E-430.83333236
GGTATCA21500.028.653491
AATCGTA405.9355945E-527.7523
ATCCACG358.8645663E-426.4285721
TATACTG451.3222102E-424.6666665
CCGTGTT400.001930145923.1259
TAAACGA400.001930145923.12516
TGCGACG400.001930145923.12522
TCTAGAC652.6793932E-622.769233
CGATCTG705.095397E-621.14285933
CGAGTCC450.00382376520.55555527
ATCGTCA450.00382376520.55555510
AACGATG450.00382376520.55555518
ATTGGAC1550.020.2903233
GTACTAG555.139263E-420.1818181
CTTTCTA555.139263E-420.18181831
CTAGACT656.895291E-519.9230774
Produced by FastQC (version 0.11.5)