##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2088233_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 607802 Sequences flagged as poor quality 0 Sequence length 43 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.889853932695186 31.0 30.0 33.0 27.0 34.0 2 30.769105728510272 31.0 31.0 34.0 27.0 34.0 3 30.75565233414829 31.0 31.0 34.0 27.0 34.0 4 34.955857335119 35.0 35.0 37.0 32.0 37.0 5 34.887356408830506 35.0 35.0 37.0 32.0 37.0 6 34.99045412815357 36.0 35.0 37.0 32.0 37.0 7 34.5769197863778 35.0 35.0 37.0 30.0 37.0 8 34.82504993402457 35.0 35.0 37.0 32.0 37.0 9 36.19914544539176 38.0 35.0 39.0 32.0 39.0 10 36.17272401209605 38.0 35.0 39.0 32.0 39.0 11 36.27043839934716 38.0 35.0 39.0 32.0 39.0 12 35.98626855456217 38.0 35.0 39.0 30.0 39.0 13 36.14691132967644 38.0 35.0 39.0 31.0 39.0 14 37.01499666009654 39.0 36.0 40.0 31.0 41.0 15 37.067721067058024 39.0 36.0 40.0 31.0 41.0 16 36.99314414891692 38.0 36.0 40.0 31.0 41.0 17 37.12020032839642 39.0 36.0 40.0 31.0 41.0 18 37.142760306810445 39.0 36.0 40.0 31.0 41.0 19 37.241387820375714 39.0 36.0 40.0 31.0 41.0 20 37.2196652857345 39.0 36.0 40.0 31.0 41.0 21 37.15519363213678 39.0 36.0 40.0 31.0 41.0 22 37.07516592574556 39.0 36.0 40.0 31.0 41.0 23 36.921237508267495 39.0 35.0 40.0 31.0 41.0 24 36.853363430854124 39.0 35.0 40.0 31.0 41.0 25 36.770120861727996 38.0 35.0 40.0 30.0 41.0 26 36.50512996008569 38.0 35.0 40.0 30.0 41.0 27 36.35503831839974 38.0 35.0 40.0 30.0 41.0 28 36.17537290104343 38.0 35.0 40.0 30.0 41.0 29 36.00140835337824 38.0 34.0 40.0 30.0 41.0 30 35.80756726697181 38.0 34.0 40.0 29.0 41.0 31 35.52093280377491 38.0 34.0 40.0 27.0 41.0 32 35.245446707973976 37.0 34.0 40.0 27.0 41.0 33 35.33400021717599 38.0 34.0 40.0 27.0 41.0 34 35.292570606875266 38.0 34.0 40.0 27.0 41.0 35 35.17859434486889 38.0 33.0 40.0 26.0 41.0 36 35.07407017416856 38.0 33.0 40.0 26.0 41.0 37 34.893924337201916 38.0 33.0 40.0 25.0 41.0 38 34.73507655453585 38.0 33.0 40.0 25.0 41.0 39 34.649869529879794 38.0 33.0 40.0 24.0 41.0 40 34.453549017607706 38.0 33.0 40.0 23.0 41.0 41 34.25291130993317 38.0 33.0 40.0 22.0 41.0 42 34.00296478129391 37.0 33.0 40.0 21.0 41.0 43 33.637607312907825 37.0 33.0 40.0 19.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 2.0 14 1.0 15 4.0 16 4.0 17 18.0 18 48.0 19 125.0 20 273.0 21 502.0 22 927.0 23 1691.0 24 2690.0 25 4175.0 26 6417.0 27 9339.0 28 13039.0 29 17155.0 30 21809.0 31 26658.0 32 32236.0 33 38830.0 34 46549.0 35 55098.0 36 67089.0 37 84530.0 38 92877.0 39 85715.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.334355596065826 25.45417751175547 14.000282986893758 21.211183905284944 2 17.9242582288311 23.130558964926077 36.198795002319834 22.74638780392299 3 20.07035185800639 26.44677049433862 32.6043678697997 20.87850977785529 4 12.827697177699315 15.612321117732419 34.73367971806608 36.82630198650219 5 10.78953343358528 41.195158949789565 34.33141055804357 13.683897058581579 6 31.557316362894493 35.422884426178264 15.137824488896054 17.881974722031188 7 23.983139246004455 36.043152210752844 21.029874860563144 18.943833682679557 8 28.229258870487428 33.01650866565098 20.032839641857052 18.721392822004535 9 24.92439972227798 13.714005547859337 24.645526010115137 36.71606871974755 10 16.53581264951415 28.29375355790208 33.34062737536237 21.8298064172214 11 30.619181904633418 21.827996617319457 25.47967923764647 22.073142240400657 12 20.452548691843724 27.970786539037384 31.42256853383174 20.15409623528715 13 31.410887098101025 21.001576171187327 29.441824804788403 18.145711925923244 14 20.85514690639386 23.112625493170473 28.99513328353642 27.037094316899253 15 23.111473802323783 28.939523068367663 27.673156718799873 20.275846410508684 16 21.719573150466765 28.346402282322202 27.69915202648231 22.234872540728723 17 18.935607319488913 27.3121839019944 28.081842442111082 25.670366336405603 18 19.99960513456685 26.37042984392944 33.427826825183196 20.20213819632051 19 19.079404148061375 24.721866660524316 35.13529076903333 21.063438422380973 20 24.29409577461081 23.315816663979387 33.656519721883114 18.73356783952669 21 23.585805903896333 24.50732310851231 32.06998331693545 19.836887670655905 22 21.488741399337286 24.234536905110545 32.887848345349305 21.38887335020286 23 20.35827456967894 26.595174086297842 34.277939197304384 18.768612146718834 24 18.66742787947391 25.56753679652255 32.40989664397287 23.355138680030667 25 20.14784419926226 26.70458471673341 33.88208660057058 19.265484483433752 26 21.76712152970869 25.37207840711284 32.21180581834216 20.64899424483631 27 20.369791478145842 27.035449044261124 31.64599655808964 20.94876291950339 28 19.99845344372016 26.530185817091752 31.73270242611903 21.73865831306906 29 18.897930576075762 26.5193270176801 32.916311561988934 21.666430844255203 30 17.98529784370568 26.91024379649952 35.344240394075705 19.7602179657191 31 19.920796575200477 26.191753235428646 33.755236080170846 20.132214109200035 32 21.176633179884238 26.634660629612934 31.23615914393174 20.952547046571087 33 22.316313536316105 26.42011707760093 30.743399988812147 20.52016939727082 34 20.143566490403124 29.753768496977635 30.761662515095377 19.341002497523867 35 18.4183336020612 30.960575977045156 31.06060855344339 19.560481867450257 36 19.7819355645424 26.62133392124409 33.6231206873291 19.973609826884413 37 19.682396569935605 27.245221305622554 32.381597954597055 20.690784169844783 38 20.163474289324483 26.80083316606395 32.89788450844189 20.137808036169673 39 21.535467142260146 26.067864205777543 32.64911928555681 19.747549366405508 40 19.590754883991828 26.963550629974893 31.507135547431563 21.938558938601716 41 20.712501768668087 26.503696927617877 31.45218344131806 21.331617862395976 42 17.81089894406402 28.394279716091752 32.003678829618856 21.79114251022537 43 18.612969355151844 28.01504437300305 31.653071230433593 21.718915041411513 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 67.0 1 73.5 2 80.0 3 290.0 4 500.0 5 500.0 6 866.0 7 1232.0 8 1409.0 9 1586.0 10 2390.0 11 3194.0 12 3194.0 13 5931.0 14 8668.0 15 13926.0 16 19184.0 17 18385.0 18 17586.0 19 17586.0 20 18543.5 21 19501.0 22 15465.5 23 11430.0 24 12114.5 25 12799.0 26 12799.0 27 14062.5 28 15326.0 29 15705.0 30 16084.0 31 17007.5 32 17931.0 33 17931.0 34 19232.5 35 20534.0 36 22251.0 37 23968.0 38 25401.0 39 26834.0 40 26834.0 41 27984.0 42 29134.0 43 34204.0 44 39274.0 45 51434.0 46 63594.0 47 63594.0 48 64984.5 49 66375.0 50 59537.0 51 52699.0 52 48690.0 53 44681.0 54 44681.0 55 38392.0 56 32103.0 57 25617.5 58 19132.0 59 16616.5 60 14101.0 61 14101.0 62 12098.0 63 10095.0 64 8622.0 65 7149.0 66 5916.5 67 4684.0 68 4684.0 69 3941.0 70 3198.0 71 2678.0 72 2158.0 73 1730.5 74 1303.0 75 1303.0 76 1010.0 77 717.0 78 541.5 79 366.0 80 304.0 81 242.0 82 242.0 83 185.0 84 128.0 85 104.0 86 80.0 87 60.0 88 40.0 89 40.0 90 38.0 91 36.0 92 20.0 93 4.0 94 3.0 95 2.0 96 2.0 97 1.5 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 607802.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.592402801590396 #Duplication Level Percentage of deduplicated Percentage of total 1 81.04800025185584 41.004130750052894 2 10.74689513807828 10.874224953842194 3 3.3569960739967355 5.095154927370012 4 1.4609690209013975 2.9565573274435453 5 0.7776775601054202 1.9672288185305722 6 0.4947402297960102 1.5018058192794683 7 0.34983823502602585 1.2389409831283904 8 0.24726155847913325 1.0007645091140245 9 0.20048845153785946 0.9128873247543468 >10 1.1575929869531025 10.636373752841134 >50 0.08636282969460812 3.0019780228510498 >100 0.05570732046972419 5.862203768099258 >500 0.010877760801780455 3.673549857865567 >1k 0.005603694958492961 6.049316667160685 >5k 9.88887345616405E-4 4.224882517666892 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 9122 1.50081770050115 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 8632 1.4201993412328358 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 7580 1.2471166597016792 No Hit GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG 4761 0.7833143030131523 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3310 0.544585243220654 No Hit ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA 2893 0.4759773742106804 No Hit ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT 2866 0.4715351380877325 No Hit GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT 2660 0.4376425217422779 No Hit ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA 2346 0.3859809609050316 No Hit TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA 2049 0.3371163635526043 No Hit CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT 1946 0.32017005537987697 No Hit GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT 1921 0.3160568737845548 No Hit GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG 1901 0.3127663285082971 No Hit CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA 1688 0.2777220213161523 No Hit CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA 1613 0.2653824765301858 No Hit GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA 1512 0.24876522288508426 No Hit TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT 1473 0.24234865959638172 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1155 0.19002898970388382 No Hit GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA 1104 0.18163809924942662 No Hit AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC 1076 0.1770313358626658 No Hit TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA 973 0.16008502768993849 No Hit ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG 967 0.15909786410706117 No Hit CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG 966 0.15893333684324829 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 892 0.1467583193210947 No Hit TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT 877 0.14429041036390142 No Hit GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC 858 0.14116439235145656 No Hit CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG 811 0.1334316109522509 No Hit AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA 775 0.12750862945498698 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 759 0.12487619323398079 No Hit CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC 710 0.11681435730714937 No Hit AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC 707 0.11632077551571071 No Hit ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC 703 0.11566266646045917 No Hit TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC 660 0.10858799411650505 No Hit CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA 646 0.10628461242312463 No Hit ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA 627 0.1031585944106798 No Hit GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA 623 0.10250048535542826 No Hit ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC 620 0.1020069035639896 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.6452726381288645E-4 0.0 11 0.0 0.0 0.0 1.6452726381288645E-4 0.0 12 0.0 0.0 0.0 3.290545276257729E-4 0.0 13 1.6452726381288645E-4 0.0 0.0 3.290545276257729E-4 0.0 14 1.6452726381288645E-4 0.0 0.0 9.871635828773186E-4 0.0 15 1.6452726381288645E-4 0.0 0.0 0.002138854429567524 0.0 16 1.6452726381288645E-4 0.0 0.0 0.003455072540070615 0.0 17 1.6452726381288645E-4 0.0 0.0 0.005593926969638139 0.0 18 1.6452726381288645E-4 0.0 0.0 0.005922981497263911 0.0 19 1.6452726381288645E-4 0.0 0.0 0.007239199607767003 0.0 20 1.6452726381288645E-4 0.0 0.0 0.0097071085649603 0.0 21 1.6452726381288645E-4 0.0 0.0 0.01250407204977937 0.0 22 1.6452726381288645E-4 0.0 0.0 0.01793347175560462 0.0 23 1.6452726381288645E-4 0.0 0.0 0.02764058032056492 0.0 24 1.6452726381288645E-4 0.0 0.0 0.04376425217422779 0.0 25 1.6452726381288645E-4 0.0 0.0 0.05116797904580768 0.0 26 1.6452726381288645E-4 0.0 0.0 0.06120414213839375 0.0 27 1.6452726381288645E-4 0.0 0.0 0.11977584805578133 0.0 28 1.6452726381288645E-4 0.0 0.0 0.18821918980194208 0.0 29 1.6452726381288645E-4 0.0 0.0 0.2596240222967348 0.0 30 1.6452726381288645E-4 0.0 0.0 0.4109891050045903 0.0 31 1.6452726381288645E-4 0.0 0.0 0.6016762037637257 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACGG 20 0.0018412537 37.0 23 GACGTCG 25 1.2329414E-4 36.999996 36 ACGTCGC 25 1.2329414E-4 36.999996 37 TGCGGCG 30 3.5975012E-4 30.833332 36 GGTATCA 2150 0.0 28.65349 1 AATCGTA 40 5.9355945E-5 27.75 23 ATCCACG 35 8.8645663E-4 26.428572 1 TATACTG 45 1.3222102E-4 24.666666 5 CCGTGTT 40 0.0019301459 23.125 9 TAAACGA 40 0.0019301459 23.125 16 TGCGACG 40 0.0019301459 23.125 22 TCTAGAC 65 2.6793932E-6 22.76923 3 CGATCTG 70 5.095397E-6 21.142859 33 CGAGTCC 45 0.003823765 20.555555 27 ATCGTCA 45 0.003823765 20.555555 10 AACGATG 45 0.003823765 20.555555 18 ATTGGAC 155 0.0 20.290323 3 GTACTAG 55 5.139263E-4 20.181818 1 CTTTCTA 55 5.139263E-4 20.181818 31 CTAGACT 65 6.895291E-5 19.923077 4 >>END_MODULE