FastQCFastQC Report
Wed 25 May 2016
SRR2088232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710655
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79651.1207970112079702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68940.9700909724127741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40390.568348917547896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27870.3921734174810562No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT14140.19897137148123914No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT11910.16759186947252885No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG11120.15647536427661804No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.1438109912686184No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA9290.1307244724936854No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.11735652320746355No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA8210.1155272248840858No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG8110.1141200723276414No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT7430.10455143494381942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA352.3870347E-531.7142872
GGTATCA17500.027.8028581
CGTATAC405.9369868E-527.7500023
CCCGTAT405.9369868E-527.7500021
ACGTTTA400.001930465523.12500226
TACTAGG603.7237107E-521.5833322
CTTAACG656.897391E-519.9230772
TAACGCT656.897391E-519.9230774
GTATCAA25800.019.8624022
CGGGCTC609.232088E-418.534
CGTTGGT500.007032306418.4999982
GGGTAGA852.7207476E-517.4117641
GCAATAC852.7207476E-517.4117643
GTAACCT752.0658677E-417.26666624
AACCGTG650.001579131317.0769237
TTAACGC1201.03951606E-716.9583323
CGCTAAG803.3802923E-416.18757
CGACGAC700.00259118815.85714325
GTAGCAC700.00259118815.8571433
GGTTAAG700.00259118815.8571431
Produced by FastQC (version 0.11.5)