FastQCFastQC Report
Wed 25 May 2016
SRR2088231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3395819
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT429731.2654679180486357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT388121.142934885516572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT344861.0155429367701871No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG232010.6832225156876736No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147050.4330325026157165No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA139740.4115060313874208No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT133380.3927771179794919No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA119990.3533462767008489No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT119490.3518738778480243No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA96340.28370181096224506No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT93110.27419011437299806No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG88590.26087962874346365No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT87990.2591127501200741No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA75410.22206719498300706No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA74450.2192401891855838No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA71830.21152481919678284No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT60390.1778363334441559No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA53410.1572816454587244No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC53160.1565454460323121No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.15159818588682142No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG48780.14364723208156854No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG47920.14111470605471021No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC43220.1272741568381589No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42940.12644961348057715No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT40510.11929375505584955No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.106395541105106No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC35240.10377467114707822No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA35000.10306791969772242No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG34430.10138938500550236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97400.029.421461
ATTGGAC13150.023.4942973
GTATTGG12400.023.1251
TATTGGA14500.021.3068962
TTGGACC22150.018.625284
TAGTACG1103.8582584E-818.54
GGACCCT22550.017.9667426
TGGACCC23400.017.946585
AGTATCA6000.017.5751
GTATCAA170550.017.453242
GTTCGCT1251.6604099E-716.27999937
CGTCTGT10050.016.01492734
GACCCTC24400.015.7704937
ACGCATA1455.3552867E-815.3103457
CGATCTG3150.015.26984133
TAGACCG855.3679856E-415.2352945
CGAACGA750.00410660314.816
CGCATAG1803.3378456E-914.3888888
TATACTG5800.014.3534485
ACCCTCG28050.013.6524078
Produced by FastQC (version 0.11.5)