##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3395819
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.81655942204222	31.0	30.0	33.0	27.0	34.0
2	30.730820164443394	31.0	31.0	34.0	27.0	34.0
3	30.755421004476386	31.0	31.0	34.0	27.0	34.0
4	34.96053411562866	35.0	35.0	37.0	32.0	37.0
5	34.88030722485504	35.0	35.0	37.0	32.0	37.0
6	34.98094892572307	35.0	35.0	37.0	32.0	37.0
7	34.597016802132266	35.0	35.0	37.0	30.0	37.0
8	34.821410092822966	35.0	35.0	37.0	32.0	37.0
9	36.227403462905414	38.0	35.0	39.0	32.0	39.0
10	36.16470076879834	38.0	35.0	39.0	32.0	39.0
11	36.256984250338434	38.0	35.0	39.0	32.0	39.0
12	36.00308555903598	38.0	35.0	39.0	30.0	39.0
13	36.163478972230266	38.0	35.0	39.0	31.0	39.0
14	37.06632980144113	39.0	36.0	40.0	31.0	41.0
15	37.115053246359714	39.0	36.0	40.0	31.0	41.0
16	37.0382617565895	39.0	36.0	40.0	31.0	41.0
17	37.14521239206212	39.0	36.0	40.0	31.0	41.0
18	37.1489902730387	39.0	36.0	40.0	31.0	41.0
19	37.233607562711676	39.0	36.0	40.0	31.0	41.0
20	37.19371644955164	39.0	36.0	40.0	31.0	41.0
21	37.1220023799855	39.0	36.0	40.0	31.0	41.0
22	37.04677339987791	39.0	36.0	40.0	31.0	41.0
23	36.92040977449034	39.0	35.0	40.0	31.0	41.0
24	36.85424252588256	39.0	35.0	40.0	31.0	41.0
25	36.776017214109466	38.0	35.0	40.0	30.0	41.0
26	36.50544772851556	38.0	35.0	40.0	30.0	41.0
27	36.36345223346709	38.0	35.0	40.0	30.0	41.0
28	36.199244718284454	38.0	35.0	40.0	30.0	41.0
29	36.03778146008371	38.0	34.0	40.0	29.0	41.0
30	35.881555230122686	38.0	34.0	40.0	29.0	41.0
31	35.62730316309556	38.0	34.0	40.0	28.0	41.0
32	35.3896706508798	38.0	34.0	40.0	27.0	41.0
33	35.50202705150068	38.0	34.0	40.0	27.0	41.0
34	35.483109376559824	38.0	34.0	40.0	27.0	41.0
35	35.3954571783714	38.0	34.0	40.0	27.0	41.0
36	35.309895492074226	38.0	34.0	40.0	27.0	41.0
37	35.1540164537627	38.0	34.0	40.0	26.0	41.0
38	35.028646697600784	38.0	33.0	40.0	25.0	41.0
39	34.98510727456322	38.0	33.0	40.0	25.0	41.0
40	34.82647514487668	38.0	33.0	40.0	24.0	41.0
41	34.66479043788848	38.0	33.0	40.0	24.0	41.0
42	34.46603337810407	38.0	33.0	40.0	23.0	41.0
43	34.13008614416729	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	4.0
15	11.0
16	32.0
17	109.0
18	283.0
19	616.0
20	1419.0
21	2788.0
22	5191.0
23	8756.0
24	14381.0
25	22844.0
26	34140.0
27	49661.0
28	69296.0
29	92542.0
30	117953.0
31	145759.0
32	176736.0
33	213991.0
34	257226.0
35	304956.0
36	370361.0
37	462234.0
38	532852.0
39	511675.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.11633688367961	25.499150573101804	13.511497520922052	21.873015022296535
2	17.78127750625107	23.109830058669203	36.24459960910755	22.864292825972175
3	19.70652735025041	26.64190877075604	32.33031560280451	21.321248276189042
4	12.86711688697189	15.935654992212484	35.477980422395895	35.71924769841973
5	11.14064088810387	40.648662369814176	34.8132512362997	13.397445505782258
6	31.629188717066487	36.07683448381672	15.388835506250482	16.905141292866315
7	24.351415667325025	35.45029932396279	21.530446705198365	18.667838303513822
8	27.639105617820032	33.6985569607803	20.26344749234279	18.398889929056878
9	24.992174200097235	13.843523462234	24.0210682607053	37.143234076963466
10	16.177422883846283	28.135097895382525	33.92406956907892	21.76340965169227
11	31.122830751580103	21.993398352503476	25.271606054386293	21.61216484153013
12	20.102690985591398	27.573524972915227	31.974554591985026	20.349229449508353
13	30.965608002075495	21.08731354645227	28.996863495963716	18.950214955508525
14	20.86400953643289	22.826599415339864	28.684184875577877	27.62520617264937
15	22.92913727145057	29.205414069477794	27.036482215335976	20.828966443735663
16	21.650241075864173	28.68998612705801	27.3811118908281	22.278660906249716
17	19.169896864349955	27.4724889636344	28.109242571526927	25.248371600488717
18	20.26948432763937	26.622532001852868	32.641610168268684	20.466373502239076
19	19.50648724210566	25.105107192108882	34.092070278186206	21.29633528759925
20	24.0907127264439	23.978103662179876	32.6298898734002	19.30129373797602
21	23.402042335000775	24.98702080411235	31.40397058853843	20.20696627234844
22	21.603212656504954	24.65643781367617	32.33697084561927	21.403378684199602
23	20.80970746674072	26.693177698811393	33.25015261414109	19.246962220306795
24	19.362574978230583	25.6687709209472	31.744595339150877	23.224058761671337
25	20.63113493387015	26.669118701556236	32.8530466435343	19.84669972103931
26	22.007503933513537	25.519793605018403	31.476471508051517	20.99623095341654
27	20.620356974267473	27.122175828570366	31.145152318188924	21.112314878973233
28	20.457745244961526	26.605275487297764	31.16049471423536	21.776484553505355
29	19.401328516036926	26.52756227584568	32.22088692006258	21.85022228805481
30	18.948713108678643	26.77354122819856	34.11739553845479	20.16035012466801
31	20.41857943547639	26.289622621229224	32.733428960730826	20.558368982563557
32	21.508596306222447	26.49472778142769	30.797695636899373	21.198980275450488
33	22.37053859466597	26.43371157296664	30.2922800066788	20.90346982568859
34	20.542879346631842	29.14675369918126	30.401914825260125	19.908452128926776
35	19.128493008608526	30.334361165892528	30.544178002420036	19.99296782307891
36	20.32263792622634	26.63946458866035	32.6514163446285	20.386481140484815
37	20.357828258808848	27.062661466939197	31.62812858989245	20.9513816843595
38	20.6830811653978	26.699302878039145	32.16799835326912	20.44961760329393
39	21.681720963337565	25.973263003711327	32.072734147491374	20.272281885459737
40	19.753437977701402	26.91268291979048	31.21732930995439	22.116549792553723
41	21.019377063382944	26.380351838540278	31.033397245259536	21.566873852817245
42	18.40737094644915	28.069900074179454	31.515755109444882	22.00697386992652
43	19.097602080676268	27.838615662377762	31.10819510698303	21.95558714996294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	364.0
1	457.0
2	550.0
3	1416.0
4	2282.0
5	2282.0
6	3922.5
7	5563.0
8	6327.0
9	7091.0
10	10814.5
11	14538.0
12	14538.0
13	26806.5
14	39075.0
15	63775.5
16	88476.0
17	85860.5
18	83245.0
19	83245.0
20	90233.5
21	97222.0
22	80327.5
23	63433.0
24	69230.5
25	75028.0
26	75028.0
27	81867.5
28	88707.0
29	94094.5
30	99482.0
31	106815.0
32	114148.0
33	114148.0
34	123195.5
35	132243.0
36	142937.0
37	153631.0
38	158200.5
39	162770.0
40	162770.0
41	169326.5
42	175883.0
43	199142.5
44	222402.0
45	277737.5
46	333073.0
47	333073.0
48	337327.0
49	341581.0
50	311966.5
51	282352.0
52	263410.5
53	244469.0
54	244469.0
55	211104.0
56	177739.0
57	145342.5
58	112946.0
59	98413.0
60	83880.0
61	83880.0
62	73108.5
63	62337.0
64	53361.0
65	44385.0
66	38082.0
67	31779.0
68	31779.0
69	26700.5
70	21622.0
71	17844.5
72	14067.0
73	11243.0
74	8419.0
75	8419.0
76	6747.5
77	5076.0
78	3970.5
79	2865.0
80	2227.0
81	1589.0
82	1589.0
83	1174.0
84	759.0
85	581.0
86	403.0
87	309.0
88	215.0
89	215.0
90	157.5
91	100.0
92	60.0
93	20.0
94	13.5
95	7.0
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3395819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.93456556765586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.40464637991161	27.022397551786376
2	14.711956205239538	11.456072470027607
3	6.194467359526931	7.235366866986161
4	3.1881828311763503	4.965220539284412
5	1.8191413489518569	3.54137390638
6	1.1118716391479366	2.5974143542333405
7	0.7419314141209323	2.022074410285655
8	0.49159115750858645	1.531191052359832
9	0.3642538788234277	1.2763859875491346
>10	1.7288288387693271	12.044823706496384
>50	0.13984015517493914	3.745180277261477
>100	0.08721460442769749	6.36068441954571
>500	0.007730918615838023	2.0669756504208396
>1k	0.006812393631541331	5.609894162800514
>5k	8.419812353590409E-4	2.4025160983022977
>10k+	6.888937380210335E-4	6.122428546280256
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	42973	1.2654679180486357	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38812	1.142934885516572	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34486	1.0155429367701871	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	23201	0.6832225156876736	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14705	0.4330325026157165	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13974	0.4115060313874208	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	13338	0.3927771179794919	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11999	0.3533462767008489	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11949	0.3518738778480243	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9634	0.28370181096224506	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	9311	0.27419011437299806	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8859	0.26087962874346365	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8799	0.2591127501200741	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7541	0.22206719498300706	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7445	0.2192401891855838	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7183	0.21152481919678284	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6039	0.1778363334441559	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5341	0.1572816454587244	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	5316	0.1565454460323121	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5148	0.15159818588682142	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4878	0.14364723208156854	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4792	0.14111470605471021	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4322	0.1272741568381589	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4294	0.12644961348057715	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4051	0.11929375505584955	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3613	0.106395541105106	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3524	0.10377467114707822	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3500	0.10306791969772242	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3443	0.10138938500550236	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.9447977056492117E-5	0.0
5	0.0	0.0	0.0	2.9447977056492117E-5	0.0
6	0.0	0.0	0.0	2.9447977056492117E-5	0.0
7	0.0	0.0	0.0	2.9447977056492117E-5	0.0
8	0.0	0.0	0.0	5.8895954112984235E-5	0.0
9	0.0	0.0	0.0	1.4723988528246058E-4	0.0
10	0.0	0.0	0.0	2.3558381645193694E-4	0.0
11	0.0	0.0	0.0	2.6503179350842904E-4	0.0
12	0.0	0.0	0.0	2.9447977056492117E-4	0.0
13	0.0	0.0	0.0	4.122716787908896E-4	0.0
14	0.0	0.0	0.0	4.4171965584738175E-4	0.0
15	0.0	0.0	0.0	6.184075181863344E-4	0.0
16	0.0	0.0	0.0	0.0012662630134291609	0.0
17	0.0	0.0	0.0	0.0018846705316154955	0.0
18	0.0	0.0	0.0	0.002326390187462877	0.0
19	0.0	0.0	0.0	0.0028859017515362275	0.0
20	0.0	0.0	0.0	0.0038871329714569593	0.0
21	0.0	0.0	0.0	0.005418427778394549	0.0
22	0.0	0.0	0.0	0.008304329529930777	0.0
23	0.0	0.0	0.0	0.013752205285381819	0.0
24	0.0	0.0	0.0	0.022616046379385944	0.0
25	0.0	0.0	0.0	0.027357170685481175	0.0
26	0.0	0.0	0.0	0.03486640483488666	0.0
27	0.0	0.0	0.0	0.08660650052314331	0.0
28	0.0	0.0	0.0	0.14146808177938813	0.0
29	0.0	0.0	0.0	0.2057824636707669	0.0
30	0.0	0.0	0.0	0.33114250200025386	0.0
31	0.0	0.0	0.0	0.49549166195253636	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9740	0.0	29.42146	1
ATTGGAC	1315	0.0	23.494297	3
GTATTGG	1240	0.0	23.125	1
TATTGGA	1450	0.0	21.306896	2
TTGGACC	2215	0.0	18.62528	4
TAGTACG	110	3.8582584E-8	18.5	4
GGACCCT	2255	0.0	17.966742	6
TGGACCC	2340	0.0	17.94658	5
AGTATCA	600	0.0	17.575	1
GTATCAA	17055	0.0	17.45324	2
GTTCGCT	125	1.6604099E-7	16.279999	37
CGTCTGT	1005	0.0	16.014927	34
GACCCTC	2440	0.0	15.770493	7
ACGCATA	145	5.3552867E-8	15.310345	7
CGATCTG	315	0.0	15.269841	33
TAGACCG	85	5.3679856E-4	15.235294	5
CGAACGA	75	0.004106603	14.8	16
CGCATAG	180	3.3378456E-9	14.388888	8
TATACTG	580	0.0	14.353448	5
ACCCTCG	2805	0.0	13.652407	8
>>END_MODULE