FastQCFastQC Report
Wed 25 May 2016
SRR2088230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211493
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT325271.4708163218242154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT295811.3376031486421165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT246251.1135011505801737No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG154250.6974925988913372No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111260.5030990376184776No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT97160.4393412052400799No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA92930.42021385552656054No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT89870.4063770493508232No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA89240.40352829513817134No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA70830.32028136647956834No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG66490.30065661523685583No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT61140.27646481358973324No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT60930.27551522885218266No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA50390.22785511869130945No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48350.21863058124081786No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45570.2060598880484813No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41170.1861638268807543No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT38550.1743166268217896No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA35950.1625598634044964No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35280.15953023590850163No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC33790.1527927061039759No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG32370.1463717045453004No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31330.1416689991783831No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29370.13280620829457745No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28020.12670173498175213No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC27390.12385298076910034No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26920.12172771968982041No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25240.11413104178941556No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA24560.11105619597258504No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA23310.10540390586811715No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA23080.10436388448889507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74050.029.9797441
GTATTGG7200.023.3819451
ATTGGAC7350.022.149663
TTGGACC12650.019.1581024
GTATCAA122400.018.6662582
GGACCCT13250.018.430196
CGTCTGT5850.018.3418834
TATTGGA9650.018.2124352
TGGACCC13900.017.9676275
ACCCGTT1054.802223E-717.61904730
GACCCTC14100.017.1879437
AGTATCA3700.017.01
GGTCGGT1554.0199666E-1016.70967923
AGTGCGC904.4489476E-516.44444510
CGGTATA1352.2222594E-816.44444326
TTTTTAC16500.015.1363621
TATACTG2350.014.9574475
GACCGTC1508.1114194E-814.822
ACCCGTG750.00410600114.87
TGCGTCT7400.014.532
Produced by FastQC (version 0.11.5)