FastQCFastQC Report
Wed 25 May 2016
SRR2088229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199476
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT304670.9522496808852451No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT275260.860328378772024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT252740.7899418529784253No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG156980.49064284276550285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110800.34630670772338973No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA87060.2721070575306706No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86940.2717319961143637No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT86470.27026300556716165No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT83180.2599800717367469No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA65760.20553365613619232No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT61410.19193767979506646No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG59750.18674933020282072No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT58950.18424892076077456No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48980.15308756808927462No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47700.1490869129820008No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46220.14446115551421546No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT39320.12289512407656755No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36850.1151751099242501No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA34290.10717379970970246No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC32920.10289184854019846No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC32800.10251678712389155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76850.028.8392961
GTATTGG11100.020.1666661
CGAACGA1205.18412E-918.516
GTATCAA133400.017.2657412
ATTGGAC14100.016.400713
TATTGGA14700.015.9829942
CGTCTGT8550.015.57894734
TGGACCC19850.014.7254415
CGTATAC1152.2121705E-514.4782613
TCTATAC2950.014.4237293
TTGGACC21100.014.3791484
GGACCCT19500.014.1358986
TATACTG4650.013.9247315
TGCGTCT9750.013.66153832
CGGTATA2850.013.63157926
TCTAGAC2457.2759576E-1213.5918363
GACCCTC21350.013.0843097
AGCCGTC2850.012.98245712
ATAGTAC5000.012.953
TAATACT6750.012.8814814
Produced by FastQC (version 0.11.5)