##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3199476
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.942593724722425	31.0	30.0	33.0	27.0	34.0
2	30.80369347980732	31.0	31.0	34.0	27.0	34.0
3	30.822252768890905	31.0	31.0	34.0	27.0	34.0
4	35.00434914967326	35.0	35.0	37.0	32.0	37.0
5	34.89694843780669	35.0	35.0	37.0	32.0	37.0
6	34.98929387187152	35.0	35.0	37.0	32.0	37.0
7	34.6143827926823	35.0	35.0	37.0	30.0	37.0
8	34.825377655591105	35.0	35.0	37.0	32.0	37.0
9	36.26950319364796	38.0	35.0	39.0	32.0	39.0
10	36.183674764242646	38.0	35.0	39.0	32.0	39.0
11	36.2649593245894	38.0	35.0	39.0	32.0	39.0
12	36.00868454709459	38.0	35.0	39.0	30.0	39.0
13	36.173482157703326	38.0	35.0	39.0	32.0	39.0
14	37.11026055516591	39.0	36.0	40.0	31.0	41.0
15	37.156705348000735	39.0	36.0	40.0	31.0	41.0
16	37.082293475556625	39.0	36.0	40.0	31.0	41.0
17	37.14553976963728	39.0	36.0	40.0	31.0	41.0
18	37.13528340265719	39.0	36.0	40.0	31.0	41.0
19	37.21106956264088	39.0	36.0	40.0	31.0	41.0
20	37.16806252023769	39.0	36.0	40.0	31.0	41.0
21	37.0971756000045	39.0	36.0	40.0	31.0	41.0
22	37.01844302004453	39.0	36.0	40.0	31.0	41.0
23	36.896536182799935	39.0	35.0	40.0	31.0	41.0
24	36.82683226878402	39.0	35.0	40.0	30.0	41.0
25	36.73951765851658	38.0	35.0	40.0	30.0	41.0
26	36.49345299042718	38.0	35.0	40.0	30.0	41.0
27	36.352139850400505	38.0	35.0	40.0	30.0	41.0
28	36.206390671472455	38.0	35.0	40.0	30.0	41.0
29	36.051645957025464	38.0	34.0	40.0	29.0	41.0
30	35.89799985997707	38.0	34.0	40.0	29.0	41.0
31	35.677563451015104	38.0	34.0	40.0	28.0	41.0
32	35.438624324733176	38.0	34.0	40.0	27.0	41.0
33	35.61087503078629	38.0	34.0	40.0	28.0	41.0
34	35.62312359898934	38.0	34.0	40.0	28.0	41.0
35	35.55839893782607	38.0	34.0	40.0	27.0	41.0
36	35.492911339231796	38.0	34.0	40.0	27.0	41.0
37	35.34695618907596	38.0	34.0	40.0	27.0	41.0
38	35.241701141061846	38.0	34.0	40.0	26.0	41.0
39	35.24315762956184	38.0	34.0	40.0	26.0	41.0
40	35.12572089929726	38.0	34.0	40.0	25.0	41.0
41	34.99465193675464	38.0	33.0	40.0	25.0	41.0
42	34.83259102428023	38.0	33.0	40.0	24.0	41.0
43	34.50840012552055	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	2.0
14	6.0
15	13.0
16	34.0
17	107.0
18	251.0
19	551.0
20	1200.0
21	2421.0
22	4499.0
23	7784.0
24	12900.0
25	20227.0
26	30731.0
27	44689.0
28	62069.0
29	84792.0
30	108590.0
31	134422.0
32	163873.0
33	199816.0
34	239303.0
35	286788.0
36	349237.0
37	433572.0
38	516957.0
39	494641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.54063102833089	24.756491375462733	12.982032057749457	22.72084553845692
2	18.132719232774367	22.81448587206155	35.65486973491909	23.397925160244988
3	19.471375937809814	26.653333233316957	31.625209878117545	22.250080950755684
4	13.271985787672733	16.17321086327886	36.00398940326478	34.550813945783624
5	11.604525241008215	40.020709641203744	35.161507696885366	13.213257420902671
6	31.763107458846385	36.71041758087887	15.668565727637901	15.857909232636844
7	25.017940437746685	34.48402175856297	22.12415408023064	18.373883723459716
8	27.170011589397763	34.054420161301415	20.34942596850234	18.42614228079848
9	25.402347134343252	13.76994232805622	23.024395244721322	37.803315292879205
10	16.08060194856908	27.65943548256027	34.28073847092462	21.979224097946037
11	32.186020460850465	22.061550078825405	24.626126278178052	21.126303182146074
12	19.98742919153011	27.24196087109264	31.768170787966532	21.002439149410716
13	30.24945334798573	21.092922716094762	28.208275355089395	20.44934858083011
14	20.872980450548777	22.505685305968854	28.06650213972538	28.55483210375699
15	23.17463859707027	29.16165022022356	26.110119282032436	21.553591900673734
16	22.156596892741188	28.367113864895376	26.776134592039448	22.700154650323988
17	19.97020762149802	27.355792010941794	27.818836584490708	24.85516378306948
18	20.967996009346532	26.72712656697534	31.140599273130974	21.16427815054715
19	20.525923619992774	25.509927250587282	32.17136181049647	21.792787318923473
20	23.922729847012448	24.709702463778445	31.096842107895167	20.27072558131394
21	23.388673645309417	25.42472579884956	30.17125304268574	21.01534751315528
22	22.212418533534866	25.105423513100273	30.87940025179123	21.802757701573633
23	21.42213287425816	26.514247958103137	31.694846281078526	20.368772886560173
24	20.385650650293986	26.02732447438268	30.394008268854023	23.19301660646931
25	21.342651109119117	26.65380206008734	31.286529419192394	20.717017411601148
26	22.323936794650123	26.02260495156082	30.167252387578465	21.486205866210593
27	21.33464979890457	27.09131120220936	29.79625413661487	21.777784862271197
28	21.18818831583672	26.594542356310846	30.11605650425257	22.101212823599862
29	20.512765215304004	26.50052696128991	30.86492913214539	22.121778691260694
30	19.947203854631194	26.77622835739352	32.29097514718035	20.98559264079493
31	21.09829859639516	26.241171991913674	31.475122801358722	21.185406610332443
32	21.73302753325857	26.469146822792233	29.81066274602466	21.987162897924538
33	22.37306984018633	26.401573257620935	29.62897674494198	21.59638015725075
34	21.31742822887248	28.30369723042148	29.575874299416526	20.80300024128951
35	20.306731477279406	29.096202003078	29.746746029662358	20.850320489980234
36	21.101330342843642	26.416325673329005	31.217611883945995	21.264732099881357
37	21.15652688127681	26.40957456783548	30.615169484003008	21.8187290668847
38	21.28145358802504	26.185631647182227	31.055585352101406	21.47732941269133
39	21.863986477785737	25.748528821594537	31.149350706178137	21.23813399444159
40	20.517859799542176	26.337500265668503	30.673897850773063	22.47074208401626
41	21.265794773894225	25.903835503063625	30.515184361439186	22.315185361602964
42	19.45928020713392	27.282842565470094	30.829892144838716	22.42798508255727
43	19.980646830918563	26.870681324066815	30.580101241578305	22.568570603436314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	213.0
1	358.0
2	503.0
3	1270.5
4	2038.0
5	2038.0
6	3408.0
7	4778.0
8	5160.5
9	5543.0
10	8581.5
11	11620.0
12	11620.0
13	20852.5
14	30085.0
15	47102.0
16	64119.0
17	63241.0
18	62363.0
19	62363.0
20	68462.0
21	74561.0
22	63150.0
23	51739.0
24	58102.0
25	64465.0
26	64465.0
27	71929.5
28	79394.0
29	85685.0
30	91976.0
31	100346.0
32	108716.0
33	108716.0
34	118938.5
35	129161.0
36	141779.0
37	154397.0
38	161584.5
39	168772.0
40	168772.0
41	176504.5
42	184237.0
43	202042.0
44	219847.0
45	257261.5
46	294676.0
47	294676.0
48	299359.0
49	304042.0
50	279562.0
51	255082.0
52	239337.0
53	223592.0
54	223592.0
55	197288.5
56	170985.0
57	145534.5
58	120084.0
59	106464.5
60	92845.0
61	92845.0
62	82220.0
63	71595.0
64	62439.5
65	53284.0
66	45137.5
67	36991.0
68	36991.0
69	31654.0
70	26317.0
71	21974.0
72	17631.0
73	14017.0
74	10403.0
75	10403.0
76	7985.5
77	5568.0
78	4485.5
79	3403.0
80	2732.5
81	2062.0
82	2062.0
83	1539.0
84	1016.0
85	802.0
86	588.0
87	497.0
88	406.0
89	406.0
90	347.5
91	289.0
92	173.5
93	58.0
94	40.5
95	23.0
96	23.0
97	14.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3199476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.578127399281755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7013475633139	31.681885862135555
2	14.333431987604364	12.492482504495268
3	5.339367883017964	6.9803896151337055
4	2.595293574276039	4.523921360733542
5	1.3451545408924113	2.930965797736593
6	0.8264336171654115	2.160865767353013
7	0.5397153228762291	1.6463848169751358
8	0.3545993271941503	1.2362219724933023
9	0.27140699137641017	1.064466760253127
>10	1.43169578641317	11.827639796324469
>50	0.153989704821848	4.703344863526166
>100	0.0968276712606329	7.825727082875759
>500	0.005077851926560519	1.534947264968632
>1k	0.0047151482174182255	4.0484407978323835
>5k	5.803259344514739E-4	1.8635845449164474
>10k+	3.6270370903217116E-4	3.4787311922467508
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30467	0.9522496808852451	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27526	0.860328378772024	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25274	0.7899418529784253	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15698	0.49064284276550285	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11080	0.34630670772338973	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8706	0.2721070575306706	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8694	0.2717319961143637	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8647	0.27026300556716165	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8318	0.2599800717367469	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6576	0.20553365613619232	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6141	0.19193767979506646	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5975	0.18674933020282072	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5895	0.18424892076077456	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4898	0.15308756808927462	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4770	0.1490869129820008	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4622	0.14446115551421546	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3932	0.12289512407656755	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3685	0.1151751099242501	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3429	0.10717379970970246	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3292	0.10289184854019846	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3280	0.10251678712389155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.125511802557669E-5	0.0	0.0	0.0	0.0
7	3.125511802557669E-5	0.0	0.0	0.0	0.0
8	3.125511802557669E-5	0.0	0.0	0.0	0.0
9	3.125511802557669E-5	0.0	0.0	3.125511802557669E-5	0.0
10	3.125511802557669E-5	0.0	0.0	9.376535407673006E-5	0.0
11	3.125511802557669E-5	0.0	0.0	1.2502047210230676E-4	0.0
12	3.125511802557669E-5	0.0	0.0	2.812960622301902E-4	0.0
13	3.125511802557669E-5	0.0	0.0	2.812960622301902E-4	0.0
14	6.251023605115338E-5	0.0	0.0	4.688267703836503E-4	0.0
15	6.251023605115338E-5	0.0	0.0	7.188677145882639E-4	0.0
16	6.251023605115338E-5	0.0	0.0	0.0015627559012788344	0.0
17	9.376535407673006E-5	0.0	0.0	0.0025316645600717115	0.0
18	9.376535407673006E-5	0.0	0.0	0.0028754708583530555	0.0
19	1.8753070815346012E-4	0.0	0.0	0.0037193590450436257	0.0
20	1.8753070815346012E-4	0.0	0.0	0.00462575746778535	0.0
21	1.8753070815346012E-4	0.0	0.0	0.006251023605115338	0.0
22	1.8753070815346012E-4	0.0	0.0	0.010720505482772804	0.0
23	1.8753070815346012E-4	0.0	0.0	0.018378009399039094	0.0
24	1.8753070815346012E-4	0.0	0.0	0.028942239291684012	0.0
25	1.8753070815346012E-4	0.0	0.0	0.03409933376590417	0.0
26	1.8753070815346012E-4	0.0	0.0	0.04103796996758219	0.0
27	1.8753070815346012E-4	0.0	0.0	0.09798479501018292	0.0
28	1.8753070815346012E-4	0.0	0.0	0.1523061901386352	0.0
29	1.8753070815346012E-4	0.0	0.0	0.2154415285503001	0.0
30	1.8753070815346012E-4	0.0	0.0	0.33149178177926636	0.0
31	1.8753070815346012E-4	0.0	0.0	0.5085520253941583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7685	0.0	28.839296	1
GTATTGG	1110	0.0	20.166666	1
CGAACGA	120	5.18412E-9	18.5	16
GTATCAA	13340	0.0	17.265741	2
ATTGGAC	1410	0.0	16.40071	3
TATTGGA	1470	0.0	15.982994	2
CGTCTGT	855	0.0	15.578947	34
TGGACCC	1985	0.0	14.725441	5
CGTATAC	115	2.2121705E-5	14.478261	3
TCTATAC	295	0.0	14.423729	3
TTGGACC	2110	0.0	14.379148	4
GGACCCT	1950	0.0	14.135898	6
TATACTG	465	0.0	13.924731	5
TGCGTCT	975	0.0	13.661538	32
CGGTATA	285	0.0	13.631579	26
TCTAGAC	245	7.2759576E-12	13.591836	3
GACCCTC	2135	0.0	13.084309	7
AGCCGTC	285	0.0	12.982457	12
ATAGTAC	500	0.0	12.95	3
TAATACT	675	0.0	12.881481	4
>>END_MODULE