FastQCFastQC Report
Wed 25 May 2016
SRR2088228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313914
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT308651.3338870848268345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT267581.1563956136658493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT244481.0565647642911533No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG186360.8053886185917023No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT103580.4476397999234198No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA102660.4436638526755964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102350.4423241313203516No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA99180.42862440004252533No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT94000.4062380883645632No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA70920.30649367262568966No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT70770.3058454203570228No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG69530.30048653493604344No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT65620.2835887591327941No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA56730.24516900800980504No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA56370.24361320256500457No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA56120.24253278211722648No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT43470.18786350745965494No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38370.16582293032498183No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38150.1648721603309371No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG36620.1582599871905352No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36350.15709313310693485No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC35470.15329005313075594No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35430.15311718585911144No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT31220.13492290551852834No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC29370.12692779420497047No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28660.12385940013328067No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA27110.1171607933570565No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC26510.11456778428238906No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG25450.10998680158380994No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG24560.10614050478971992No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24320.10510330115985297No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA23980.10363392935087476No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA23800.10285602662847453No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.10233742481354102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69600.029.9827581
GTATTGG8900.025.5674151
ATTGGAC9800.024.5408173
GACGCGA400.001931775123.12500233
TATTGGA11850.020.7637122
TTGGACC16400.020.0792684
ACGGTGT1052.262459E-819.38095329
ACGTTTA1254.129106E-1019.2426
GCCGTCA1355.638867E-1119.18518613
TGGACCC16800.019.1607155
CGGATCG801.6178241E-518.524
GTGACCG701.2198754E-418.59
TATACCG1002.8780596E-718.4999985
GGACCCT17700.017.872886
GTATCAA122450.017.691712
CGTCTGT6200.017.60483734
CGACGAG1107.8160156E-716.81818224
AGTATCA4150.016.4939771
CCGTATA904.4490902E-516.4444452
CAATGCG1352.2224413E-816.44444519
Produced by FastQC (version 0.11.5)