##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2313914
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05983411656613	31.0	30.0	33.0	27.0	34.0
2	30.855524881218575	31.0	31.0	34.0	27.0	34.0
3	30.81466683722904	31.0	31.0	34.0	27.0	34.0
4	34.99253386253768	35.0	35.0	37.0	32.0	37.0
5	34.913126849139594	35.0	35.0	37.0	32.0	37.0
6	35.01717738861513	36.0	35.0	37.0	32.0	37.0
7	34.62532574676501	35.0	35.0	37.0	30.0	37.0
8	34.85637754903596	35.0	35.0	37.0	32.0	37.0
9	36.25887003579217	38.0	35.0	39.0	32.0	39.0
10	36.20653533363816	38.0	35.0	39.0	32.0	39.0
11	36.3007894848296	38.0	35.0	39.0	32.0	39.0
12	36.039058063523534	38.0	35.0	39.0	30.0	39.0
13	36.203043846919115	38.0	35.0	39.0	32.0	39.0
14	37.110903862459885	39.0	36.0	40.0	31.0	41.0
15	37.15124589764356	39.0	36.0	40.0	31.0	41.0
16	37.06794677762441	39.0	36.0	40.0	31.0	41.0
17	37.17616514701929	39.0	36.0	40.0	31.0	41.0
18	37.17250122519679	39.0	36.0	40.0	31.0	41.0
19	37.25898672120053	39.0	36.0	40.0	31.0	41.0
20	37.215872327147856	39.0	36.0	40.0	31.0	41.0
21	37.14760401639819	39.0	36.0	40.0	31.0	41.0
22	37.068155947023094	39.0	36.0	40.0	31.0	41.0
23	36.9270029050345	39.0	35.0	40.0	31.0	41.0
24	36.853258159119136	39.0	35.0	40.0	31.0	41.0
25	36.77399376122017	38.0	35.0	40.0	30.0	41.0
26	36.50873887274981	38.0	35.0	40.0	30.0	41.0
27	36.3642447385685	38.0	35.0	40.0	30.0	41.0
28	36.20152045408775	38.0	35.0	40.0	30.0	41.0
29	36.02745478008258	38.0	35.0	40.0	29.0	41.0
30	35.86093735549377	38.0	34.0	40.0	29.0	41.0
31	35.5972737102589	38.0	34.0	40.0	28.0	41.0
32	35.361130966838004	38.0	34.0	40.0	27.0	41.0
33	35.47531628228188	38.0	34.0	40.0	27.0	41.0
34	35.44714064567655	38.0	34.0	40.0	27.0	41.0
35	35.3432495762591	38.0	34.0	40.0	27.0	41.0
36	35.25624288543135	38.0	34.0	40.0	26.0	41.0
37	35.09625336118801	38.0	33.0	40.0	25.0	41.0
38	34.96624809737959	38.0	33.0	40.0	25.0	41.0
39	34.91245309894836	38.0	33.0	40.0	25.0	41.0
40	34.74434313461953	38.0	33.0	40.0	24.0	41.0
41	34.571478455984106	38.0	33.0	40.0	23.0	41.0
42	34.35730325327562	38.0	33.0	40.0	23.0	41.0
43	34.01809920334118	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	4.0
15	9.0
16	23.0
17	70.0
18	207.0
19	417.0
20	978.0
21	1880.0
22	3449.0
23	6006.0
24	9650.0
25	15469.0
26	23363.0
27	33442.0
28	47100.0
29	62524.0
30	80535.0
31	98708.0
32	120158.0
33	144846.0
34	175046.0
35	208171.0
36	253056.0
37	318043.0
38	368805.0
39	341952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.87447243069535	24.81336817185081	13.313632226608249	21.998527170845588
2	18.095357044384535	22.764113100141145	36.06015608185957	23.08037377361475
3	20.11570006491166	26.61810248781934	31.958102159371528	21.308095287897476
4	12.93099052082316	15.6239168785011	34.99239816172943	36.4526944389463
5	11.199940879393097	40.59554503754245	34.65146068522858	13.553053397835873
6	31.976339656530016	35.33190948323922	15.310854249552921	17.380896610677837
7	24.632721872982316	35.304293936594014	21.153379079775654	18.90960511064802
8	28.106619347132177	33.2618671221143	20.02870461045657	18.60280892029695
9	25.154564949259132	13.515325115799465	23.829018710289144	37.50109122465226
10	16.46145880961868	27.61805322064692	33.5881973141612	22.332290655573196
11	31.22574996304962	21.26820616496551	25.3366374031187	22.169406468866175
12	20.3233136581567	27.11933114195255	31.790420905876367	20.766934294014384
13	31.440926499429107	20.453525930522915	29.12009694396594	18.98545062608204
14	20.99520552622094	22.51241835262676	28.291155159612675	28.201220961539626
15	23.286604428686633	28.481136291149973	27.146168785875364	21.086090494288033
16	22.019487327532484	28.2345411281491	27.1548553662755	22.591116178042917
17	19.383996120858423	26.712444801319325	27.804015188118488	26.099543889703764
18	20.72216167065846	26.00995542617401	32.49943602052626	20.76844688264127
19	19.68448265579447	24.282838515173857	33.904890155813916	22.12778867321776
20	24.810991246865697	23.116070865209338	32.52052582766689	19.552412060258074
21	23.95789126130012	24.185384590784274	31.196146442780503	20.660577705135108
22	22.12273230552216	23.767477961583705	32.2664973719853	21.843292360908833
23	21.10013596010915	25.911161780429175	33.454916647723294	19.53378561173838
24	19.66412753455833	24.828839792662993	31.610206775186978	23.8968258975917
25	20.965774873223463	25.981864494531777	32.813017251289374	20.239343380955386
26	22.531347318871834	24.840076165319886	31.289278685378974	21.339297830429306
27	21.089591056538833	26.53270605562696	30.766268755018555	21.611434132815653
28	20.78335668482061	25.71404987393654	31.04990073096926	22.45269271027359
29	19.72493359735928	25.764786418164203	32.19696151196631	22.31331847251021
30	19.018597925419872	26.066742324909224	34.26912149716886	20.64553825250204
31	20.692082765392318	25.45993498461914	32.88592402310544	20.96205822688311
32	22.031026217914754	25.661152488813325	30.433326389831254	21.874494903440663
33	23.061099072826387	25.502676417533237	30.005868843872328	21.430355665768044
34	20.958860182357686	28.846145535227325	30.074410717079374	20.120583565335618
35	19.461656742644713	30.04044229820123	30.248012674628356	20.2498882845257
36	20.687372132240007	25.843052075401246	32.68561407208738	20.783961720271368
37	20.745758053237935	26.36238857623922	31.590543123037417	21.301310247485432
38	21.065735373051893	25.843484243580356	32.149423012264066	20.94135737110368
39	22.273342915942425	25.124745344900457	32.02737871848306	20.57453302067406
40	20.254771784949657	26.296526145742664	30.807497599305766	22.64120447000191
41	21.568044447632886	25.744949898743	30.62373968954767	22.063265964076454
42	18.76353226610842	27.69480628925708	31.206734563168727	22.334926881465776
43	19.650341369644682	27.41878047325873	30.618596888216242	22.312281268880348
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	179.0
1	249.5
2	320.0
3	880.5
4	1441.0
5	1441.0
6	2550.5
7	3660.0
8	4121.0
9	4582.0
10	7135.0
11	9688.0
12	9688.0
13	17958.5
14	26229.0
15	43068.0
16	59907.0
17	58262.5
18	56618.0
19	56618.0
20	61355.0
21	66092.0
22	53469.0
23	40846.0
24	43974.0
25	47102.0
26	47102.0
27	51276.5
28	55451.0
29	57415.5
30	59380.0
31	63225.0
32	67070.0
33	67070.0
34	72405.5
35	77741.0
36	84400.5
37	91060.0
38	95545.0
39	100030.0
40	100030.0
41	105598.5
42	111167.0
43	129296.5
44	147426.0
45	191297.5
46	235169.0
47	235169.0
48	239433.0
49	243697.0
50	222990.5
51	202284.0
52	190466.5
53	178649.0
54	178649.0
55	154557.5
56	130466.0
57	106800.5
58	83135.0
59	73021.0
60	62907.0
61	62907.0
62	54904.0
63	46901.0
64	40860.0
65	34819.0
66	29756.0
67	24693.0
68	24693.0
69	20789.0
70	16885.0
71	14486.0
72	12087.0
73	9596.5
74	7106.0
75	7106.0
76	5607.0
77	4108.0
78	3147.5
79	2187.0
80	1736.0
81	1285.0
82	1285.0
83	963.0
84	641.0
85	505.0
86	369.0
87	317.0
88	265.0
89	265.0
90	236.5
91	208.0
92	123.5
93	39.0
94	29.0
95	19.0
96	19.0
97	11.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2313914.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.17414262834375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.71008533261008	31.508337945932297
2	13.621145369487142	11.489202551483094
3	4.9066648541715105	6.208031501679324
4	2.249861796285896	3.7954396916249222
5	1.1503848070517912	2.4258246465040973
6	0.6851756114633407	1.7338016377990546
7	0.4546908078215196	1.3423336486603126
8	0.3178282570248675	1.072330739446773
9	0.22505829383388273	0.854247652545768
>10	1.4193344743741487	11.548721574353642
>50	0.1527417785052482	4.481319512928798
>100	0.09099755427342934	7.07777029695739
>500	0.007390944674672241	2.1686009666832495
>1k	0.006974553425394931	5.711565308174563
>5k	9.368803108739459E-4	2.8064144504465958
>10k+	7.286846862352913E-4	5.776057874780315
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30865	1.3338870848268345	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26758	1.1563956136658493	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24448	1.0565647642911533	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18636	0.8053886185917023	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10358	0.4476397999234198	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10266	0.4436638526755964	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10235	0.4423241313203516	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9918	0.42862440004252533	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9400	0.4062380883645632	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7092	0.30649367262568966	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7077	0.3058454203570228	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6953	0.30048653493604344	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6562	0.2835887591327941	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5673	0.24516900800980504	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5637	0.24361320256500457	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5612	0.24253278211722648	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4347	0.18786350745965494	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3837	0.16582293032498183	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3815	0.1648721603309371	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3662	0.1582599871905352	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3635	0.15709313310693485	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3547	0.15329005313075594	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3543	0.15311718585911144	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3122	0.13492290551852834	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2937	0.12692779420497047	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2866	0.12385940013328067	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2711	0.1171607933570565	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2651	0.11456778428238906	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2545	0.10998680158380994	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	2456	0.10614050478971992	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2432	0.10510330115985297	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2398	0.10363392935087476	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2380	0.10285602662847453	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2368	0.10233742481354102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.2965045373337125E-4	0.0	0.0	0.0	0.0
7	1.2965045373337125E-4	0.0	0.0	0.0	0.0
8	1.2965045373337125E-4	0.0	0.0	0.0	0.0
9	1.2965045373337125E-4	0.0	0.0	8.64336358222475E-5	0.0
10	1.2965045373337125E-4	0.0	0.0	8.64336358222475E-5	0.0
11	1.2965045373337125E-4	0.0	0.0	2.1608408955561874E-4	0.0
12	1.2965045373337125E-4	0.0	0.0	2.593009074667425E-4	0.0
13	1.2965045373337125E-4	0.0	0.0	3.4573454328899E-4	0.0
14	1.2965045373337125E-4	0.0	0.0	3.889513612001137E-4	0.0
15	1.2965045373337125E-4	0.0	0.0	9.507699940447225E-4	0.0
16	1.2965045373337125E-4	0.0	0.0	0.002031190441822816	0.0
17	1.2965045373337125E-4	0.0	0.0	0.003759863158267766	0.0
18	1.2965045373337125E-4	0.0	0.0	0.00414881451946788	0.0
19	1.2965045373337125E-4	0.0	0.0	0.005099584513512602	0.0
20	1.2965045373337125E-4	0.0	0.0	0.006396089050846315	0.0
21	1.2965045373337125E-4	0.0	0.0	0.008254412221024637	0.0
22	1.2965045373337125E-4	0.0	0.0	0.01452085081813758	0.0
23	1.2965045373337125E-4	0.0	0.0	0.02454715257351829	0.0
24	1.2965045373337125E-4	0.0	0.0	0.037944366125966654	0.0
25	1.2965045373337125E-4	0.0	0.0	0.044037937451435104	0.0
26	1.2965045373337125E-4	0.0	0.0	0.051557663767970635	0.0
27	1.2965045373337125E-4	0.0	0.0	0.10436861525536385	0.0
28	1.72867271644495E-4	0.0	0.0	0.1594268412741355	0.0
29	1.72867271644495E-4	0.0	0.0	0.22520283813486586	0.0
30	2.1608408955561874E-4	0.0	0.0	0.35658196458468205	0.0
31	3.0251772537786626E-4	0.0	0.0	0.524522518987309	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6960	0.0	29.982758	1
GTATTGG	890	0.0	25.567415	1
ATTGGAC	980	0.0	24.540817	3
GACGCGA	40	0.0019317751	23.125002	33
TATTGGA	1185	0.0	20.763712	2
TTGGACC	1640	0.0	20.079268	4
ACGGTGT	105	2.262459E-8	19.380953	29
ACGTTTA	125	4.129106E-10	19.24	26
GCCGTCA	135	5.638867E-11	19.185186	13
TGGACCC	1680	0.0	19.160715	5
CGGATCG	80	1.6178241E-5	18.5	24
GTGACCG	70	1.2198754E-4	18.5	9
TATACCG	100	2.8780596E-7	18.499998	5
GGACCCT	1770	0.0	17.87288	6
GTATCAA	12245	0.0	17.69171	2
CGTCTGT	620	0.0	17.604837	34
CGACGAG	110	7.8160156E-7	16.818182	24
AGTATCA	415	0.0	16.493977	1
CCGTATA	90	4.4490902E-5	16.444445	2
CAATGCG	135	2.2224413E-8	16.444445	19
>>END_MODULE