FastQCFastQC Report
Wed 25 May 2016
SRR2088227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088939
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT365581.1835131739409552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT317061.026436585507192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT289720.9379272300294696No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG218340.7068446479519344No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA131960.4272017025910839No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA124930.4044430789989702No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123560.4000078991524274No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT121530.39343606332141884No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT111960.36245455154666373No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA88030.2849845853220151No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG86930.281423492014572No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT82490.26704962448271075No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT76040.24616866827088524No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA69800.22596755714502617No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA69280.22428413121787122No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA64650.209295165751088No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT50550.16364842426477183No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA48190.15600826044153024No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG45660.1478177458344111No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC44790.14500124476397883No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG43470.1407279327950471No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC42710.13826754105535916No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42410.13729633378969283No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT38620.12502674866677524No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC37320.12081818384888793No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34810.1126924163928132No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA32600.10553785620240477No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC31530.1020738836215283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84750.029.3598821
GTATTGG12050.021.1867221
ATTGGAC11650.020.8025743
TATACCG2350.018.8936185
GGACCCT19350.018.0697676
GTATCAA143250.017.989882
TTGGACC19250.017.8753244
TATTGGA14550.017.164952
TGGACCC20950.016.6897375
CGTCTGT7800.016.36538534
TGTTACG700.00259382215.85714216
GACCCTC22150.015.3679467
GGTATCG1406.003811E-714.53571435
CACGGTA1304.449521E-614.23076932
AGTATCA5350.014.1775711
TTTTTAC17600.014.0852271
TAGTGCG800.006301795613.8757
TTACGGC1203.3039047E-513.87499918
TATCGGC1203.3039047E-513.87499937
CGCCGGA2950.013.7966114
Produced by FastQC (version 0.11.5)