FastQCFastQC Report
Wed 25 May 2016
SRR2088226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269579
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT426261.3037152489663042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT379301.1600881948409871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT340431.0412043874761858No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG245850.7519316707135689No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155650.47605517407592846No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA148080.4529023461430356No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT139710.42730272001379993No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA139670.42718038010398285No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT127890.3911512766628364No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA97830.2992128344352591No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG95970.2935240286287623No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT93160.2849296499641085No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT89300.27312384866675493No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA79060.24180483175356826No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA78310.23951095844449696No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA75800.23183412910347173No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT56710.17344740714324383No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA54780.16754450649456704No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC50280.1537812666401393No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49980.15286371731651077No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG49670.15191558301542799No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG49430.15118154355652516No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC49240.15060042898489379No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT42920.13127072323378638No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC40350.1234103840280354No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA40200.12295160936622115No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA38380.11738514346954149No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36600.11194101748267897No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.1094330493314277No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC35760.10937187937651913No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG34470.1054264172849165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98200.029.1817721
GTATTGG13800.021.9855081
ATTGGAC14950.021.4080283
CGCGATA701.22007084E-418.514
TTGGACC23300.018.0236054
TATTGGA17750.017.9267622
GTATCAA169350.017.5659892
TGGACCC23450.017.4349695
ACGTTTA2250.017.26666626
GGACCCT23700.017.2510556
CGTCTGT9500.015.96842134
CGATCTG3300.015.6969733
GACCCTC25700.015.404677
CGTATAC1701.4897523E-915.2352943
GAACCGT1353.9783663E-715.0740756
TAAACGT1752.242814E-914.7999994
ATCACGT2400.014.64583323
AGTATCA7100.014.5915491
TGCGACG1406.00412E-714.53571422
CTTGCGA1152.2121998E-514.47826110
Produced by FastQC (version 0.11.5)